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Among various histones, histone H1 proteins have been appreciated for their multiple functions in diverse biological processes. In addition to being a structural protein in chromatin, H1 proteins also play critical roles in cell cycle, gene expression, and development. Recent studies reveal the possible effects of H1 in some diseases, such as cancer and neurodegenerative diseases. Here, we review different variants of HI, the functions, and post translational modifications of ill variants are also discussed. 相似文献
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调节大豆籽粒生育的生理生态因素 总被引:1,自引:0,他引:1
大豆籽粒是生殖生长时期植株光合产物主要存储库,鼓粒灌浆期籽粒的生长发育以及干物质积累的过程直接影响大豆的产量。因此,研究大豆籽粒的生长发育及其影响因素对提高产量至关重要。影响大豆籽粒生长发育的因素很多,从源库的改变、外界温度、光照、水分供应以及二氧化碳的浓度的影响,酶活性、内源物质含量的调节以及植物激素的调控等方面予以综述。参64。 相似文献
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本研究首次利用诱导性多能干细胞(iPS细胞)通过四倍体囊胚注射得到存活并具有繁殖能力的小鼠。研究中,我们制备了37株iPS细胞系,其中3株iPS细胞系获得了共计27个活体小鼠,经多种分子生物学技术鉴定,证实该小鼠确实从iPS细胞发育而成。其中一些小鼠现已发育成熟并且繁殖了后代,这是世界上第一次获得完全由iPS细胞制备的活体小鼠,有力地证明了iPS细胞具有真正的全能性。这项工作为进一步研究iPS技术在干细胞、发育生物学和再生医学领域的应用提供了技术平台,将iPS细胞研究推到了新的高度,也为中国在这一国际热点研究领域取得领先地位做出了重要的贡献。 相似文献
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Human peripheral blood monuclear cells transfected with messenger RNA stimulate antigen-specific cytotoxic T-lymphocytes in vitro 总被引:1,自引:0,他引:1
Teufel R Carralot JP Scheel B Probst J Walter S Jung G Hoerr I Rammensee HG Pascolo S 《Cellular and molecular life sciences : CMLS》2005,62(15):1755-1762
The efficiency of test vaccines needs to be evaluated by quantification of the triggered cellular immune response. Usually, for these assays, autologous target cells expressing the vaccine antigen are required. In the context of messenger RNA (mRNA)-based vaccinations, the target cells used for the read-out are mRNA-transfected monocyte-derived dendritic cells (Mo-DCs). Their production typically requires samples of 100 ml blood from the patients, and limits the number of assays that can be performed. We show here that fresh peripheral blood mononuclear cells (PBMCs) can be transfected with mRNA by electroporation. Such cells are as efficient as mRNA-transfected Mo-DCs for their ability to activate memory T cells in vitro. Thus, mRNA-transfected PBMCs are a convenient replacement of mRNA-transfected Mo-DCs for the in vitro monitoring of natural or vaccine-induced immune responses.Received 17 February 2005; received after revision 1 May 2005; accepted 7 Juni 2005 相似文献
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Marrow mesenchymal stem cells transduced with TPO/FL genes as support for ex vivo expansion of hematopoietic stem/progenitor cells 总被引:4,自引:0,他引:4
Xie CG Wang JF Xiang Y Jia BB Qiu LY Wang LJ Wang GZ Huang GP 《Cellular and molecular life sciences : CMLS》2005,62(21):2495-2507
A new marrow-derived mesenchymal stem cell (hMSC) line that could support expansion of hematopoietic stem/progenitor cells
(HSPCs) was developed. Primary hMSCs were infected with retrovirus containing Flt-3 ligand and thrombopoietin genes. CD34+
cells from cord blood were expanded with primary hMSCs or transduced hMSCs. The expansion of total nucleated cells, CD34+
cells and mixed colonies containing erythroid and myeloid cells and megakaryocytes for 2 weeks coculture with transduced hMSCs
was remarkably increased. The outputs of long-term culture-initiating cells for 2 and 4 weeks coculture with transduced hMSCs
were also largely increased. The expansion rates of HSPCs with transduced hMSCs were unchanged for 6 weeks. In contrast, the
expansion rates of HSPCs with primary hMSCs declined drastically through 6 weeks. SCID-repopulating cell expansion with transduced
hMSCs for 4 weeks was significantly higher than that of uncultured CD34+ cells and HSPCs expanded with primary hMSCs.
Received 21 June 2005; received after revision 30 July 2005; accepted 24 August 2005 相似文献
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Di Felice V David S Cappello F Farina F Zummo G 《Cellular and molecular life sciences : CMLS》2005,62(1):4-9
Heat shock protein 60 (HSP60) plays an important role in the protein folding of prokaryotic and eukaryotic cells. Most of the papers published on chlamydial HSP60 concern its role in immune response during infection. In the last decade, exposure to Chlamydia trachomatis has been consistently associated with the development of cervical and ovarian cancer. Moreover, it has been suggested that chlamydial HSP60 may have an anti-apoptotic effect during persistent infection. We hypothesize that the accumulation of exogenous chlamydial HSP60 in the cytoplasm of actively replicating eukaryotic cells may interfere with the regulation of the apoptotic pathway. The concomitant expression of viral oncoproteins and/or the presence of mutations may lead to the ability to survive apoptotic stimuli, loss of replicative senescence, uncontrolled proliferation and, finally neoplastic transformation.Received 15 August 2004; received after revision 1 October 2004; accepted 7 October 2004 相似文献