黑曲霉发酵粉中一种β-葡萄糖苷酶的分离纯化与表征

黑曲霉发酵酶粉通过硫酸铵分级沉淀、SephadexG-25脱盐、再通过两步DEAE-TOYOPEARL650M弱阴离子交换色谱,分离得到一个新的电泳纯的β-葡萄糖苷酶.该酶对水杨素的比活力为597.12IU mg,并对其专一,不能水解棉花和羧甲基纤维素钠;DTT处理前后分子量均为117.5kDa;最适pH和温度分别为4 5和70℃;在pH5.0、50℃下对水杨素钠的米氏常数Km为3.73mg mL,最大反应速率Vm为0.088mg葡萄糖 (mL·min).  

普通高校仪器分析实验教学的探索

根据目前国内仪器分析实验课程的发展现状和存在的问题，从组织实验教学、提高预习质量、精选实验内容、与有机化学相结合、开放式设计实验、科研促教学等方面对仪器分析实验教学进行了探索与实践，有利于强化教师的业务素质和科研能力，有利于培养学生的综合素质和创新意识，有利于提高教学质量和教学效果。  

拉张槽对四川盆地海相油气分布的控制作用

四川盆地现今为挤压性的沉积盆地，地表所见构造也均为压性构造；但四川盆地沉积盖层在形成、演化过程中发生了拉张运动（地裂运动）并形成了张性构造（拉张槽等）。现今盆地内已发现的主要拉张槽有开江－梁平海槽（拉张槽）和绵阳－长宁拉张槽。开江－梁平海槽（拉张槽）是四川盆地内深埋地下的二叠纪－三叠纪之间的一个北西－南东向展布的深水碳酸盐岩沉积区，是在峨眉地裂运动理论启示下因生物礁油气藏勘探而发现的。绵阳－长宁拉张槽是四川盆地内发育于早寒武世的一个南北向的下寒武统巨厚碎屑岩沉积（降）区，是在兴凯地裂运动理论指导下通过构造-沉积综合研究而发现的。2个拉张槽的发现和特征研究均经历了漫长过程。拉张槽的形成演化不仅控制了优质泥质烃源岩和优质碳酸盐岩储集岩的发育，而且为优质泥质烃源岩-优质碳酸盐岩储集岩组合的形成和油气成藏效应及规模的提高创造了条件，致使环拉张槽周缘地区是克拉通盆地内油气分布最丰富的地区。  

Vegetational ecotype of the Gyirong Basin in Tibet, China and its response in stable carbon isotopes of mammal tooth enamel

Carbon isotope analysis of modern herbaceous plants in the Gyirong Basin (Tibet, China) indicates that although C3 plants are dominant, C4 plants rarely comprise of the vegetation in the area at 4000 m above sea level. The C4 plants discovered in the Gyirong Basin are Salsola nepalensis of Chenopodiaceae and Pennisetum flaccidum of Gramineae, affirming that C4 plants affected by high solar gain can be distributed at high altitude, which supports the opinion that some C4 plants can exist in areas of high elevation. Carbon isotope analysis of herbivore tooth enamel from the Gyirong Basin indicates that carbon isotopes of structural carbonate in biogenic apatite at high altitude still keep a stable enrichment relationship with those of plants in their diet. Carbon isotopes in tooth enamel are therefore an accurate proxy for vegetation ecotypes and should reflect climatic and environmental features.  

我国医学期刊的发展现状、问题与展望

我国医学期刊经过近百年的发展，在期刊的数量、种类、学科分布方面取得了不小成就，但在管理体制、学术质量、编辑人才等方面尚存在不少问题，医学期刊的规范化、电子化及规模化经营是我国医学期刊日后的发展趋势．  

事故树分析法在矿井水害防治中的应用

利用事故树分析方法,找出了影响矿井突水的主要因素;绘制了矿井突水事故树图,求出了其最小割集、最小径集和各基本事件的结构重要度;阐述了各基本事件对引起事故发生的影响程度,最后通过计算机编程实现了定性分析和定量分析.针对矿井突水事故发生的主要原因提出了所需采取的预防措施,为矿区水害防治工作提供了有力支持.  

A mammalian fossil from the Dingqing Formation in the Lunpola Basin, northern Tibet, and its relevance to age and paleo-altimetry

The distal extremity of a rhinocerotid humerus from the upper part of the Dingqing Formation at the Lunbori locality in Baingoin County, northern Tibet, is the first mammalian fossil found in Cenozoic deposits of the Lunpola Basin. The medial condyle of the distal trochlea of the humerus specimen from Lunbori gradually contracts from medially to laterally. The margin of the medial surface of the medial condyle is not prominent, the well-developed medial epicondyle strongly extends posteriorly, and is divided from the articular facet of the medial condyle by a groove; all of which are characteristic for the Rhinocerotidae. The medial condyle is wide at the bottom and narrow at the top. The medial collateral ligament fossa is relatively shallow, and the medial collateral ligament tubercle is very weak. The medial part of the upper margin of the medial condyle smoothly connects to the bony surface above, but there is no clear boundary between them. All of these characteristics are identical with those of Plesiaceratherium. These comparisons imply that the Lunbori specimen is closest to Plesiaceratherium gracile in the Shanwang Fauna from Linqu, Shandong Province, in size and morphology. Thus, its age is suggested to be the late Early Miocene (Shanwangian Age), about 18?C16 Ma. Discovery of the rhinocerotid fossil suggests that the upper part of the Dingqing Formation deposited in the Neogene. While adjusting to paleo-temperatures of the Early Miocene, a paleo-ecosystem reconstruction indicates that the paleo-elevation was close to 3000 m in the Lunpola Basin during this time.  

某型发动机高空、低速下燃烧室冷却性能分析

通过对某型发动机燃烧室火焰筒在高空、低速条件下的流量分配、沿程参数和壁温分布计算，对该型发动机燃烧室的冷却性能进行分析研究。结果表明：在高空条件下，火焰筒同一轴向位置的壁温随马赫数的增大而升高；气膜冷却有一定的冷却保护有效长度，使得火焰筒壁温沿轴向分布呈锯齿形。  

邛海冬候鸟的监测及保护

研究了邛海冬候鸟的组成，种群相对数量及其优势度，分析出冬候鸟在不同年份的分类阶元和优势度的相似性系数变小，冬候鸟渐趋贫乏，并提出相应的保护建议。  

凹坑深度对表面动态弹流润滑的影响

把一种快速求解非稳态弹流润滑方程的数值方法应用于微弹流润滑分析，研究了几种深度的表面凹坑在三种滚滑接触运动条件下的压力波动和油膜变化特征，指出不仅运动条件变化带来不同的润滑特征，而且不同深度的凹坑在不同运动条件下对润滑扰动的程度也不同．研究结果适用于零件表面点蚀后的润滑状态分析．