粗毛栓菌漆酶基因的克隆、序列分析及荧光定量PCR分析

本研究通过提取不同培养条件下的粗毛栓菌总RNA,利用RT-PCR的方法克隆到1个新的漆酶基因,命名为Tglac3,编码区长1,560bp,预测编码519个氨基酸残基,其分子量为56.6kDa,理论等电点为5.77.用荧光定量RT-PCR法分析了该漆酶基因在不同培养条件下的表达水平,结果显示,高浓度的碳源和氮源均能诱导Tglac3基因的表达.     

Remote Visualization of High-Speed Rail Power Quality Monitoring

The visualization techniques were explored for power quality monitoring.And remote visualization solutions were proposed for highspeed rail power quality monitoring.Taking the Beijing-Shanghai highspeed rail power monitoring as a study case,a remote visualization client,based on our proposed solutions,was developed for high-speed rail power quality monitoring to efficiently support power quality data analysis of the electricity business.The solutions collected data from monitoring stations deployed along the high-speed rail route and visualized the data set with a variety of visualization technologies to alert the specific stations of catastrophic events.The proposed solutions have been proved to be effective in supporting decision-making for the railway power scheduling and providing diagnosis information for quickly spotting any possible runtime failure in operation.     

政府采购的审计思考

政府采购已成为公共财政支出的一项重要制度。近年来,我国各级政府部门陆续开展了一些政府采购活动,但从整体情况看,我国的政府采购工作尚处于推行发展阶段,面对实际,如何有效发挥审计的监督和服务作用,是一个现实而迫切的问题。本文通过理论联系实际,就政府采购审计的若干问题展开研究,力争在实用性和可操作性上有所突破,以便为审计部门更好履行审计职责提供决策依据。     

运动发酵单胞菌RecET重组系统的构建及应用

为了提高外源基因整合到染色体的效率,在已构建的运动发酵单胞菌-大肠杆菌穿梭载体基础上,构建了RecET表达质粒pSUZM3a-RecET.选取乙醇脱氢酶Ⅰ(adhA)基因作为靶基因,四环素抗性基因(Tcr)作为筛选标记基因,检测RecET重组系统在Z.mobilis中进行基因重组的可行性.将两端带有60bp adhA基因同源臂的四环素抗性基因片段电击转化含有RecET重组系统表达质粒的运动发酵单胞菌ZM4,获得adhA基因缺失突变菌株.对突变菌株adhA基因的PCR产物进行测序发现,adhA基因已被置换为四环素抗性基因.上述结果表明:RecET重组系统在运动发酵单胞菌中具有高效、便捷和可操作性,只需60bp同源臂即可完成同源重组.