Drosophila Piwi functions in Hsp90-mediated suppression of phenotypic variation

Canalization, also known as developmental robustness, describes an organism's ability to produce the same phenotype despite genotypic variations and environmental influences. In Drosophila, Hsp90, the trithorax-group proteins and transposon silencing have been previously implicated in canalization. Despite this, the molecular mechanism underlying canalization remains elusive. Here using a Drosophila eye-outgrowth assay sensitized by the dominant Kr(irregular facets-1)(Kr(If-1)) allele, we show that the Piwi-interacting RNA (piRNA) pathway, but not the short interfering RNA or micro RNA pathway, is involved in canalization. Furthermore, we isolated a protein complex composed of Hsp90, Piwi and Hop, the Hsp70/Hsp90 organizing protein homolog, and we demonstrated the function of this complex in canalization. Our data indicate that Hsp90 and Hop regulate the piRNA pathway through Piwi to mediate canalization. Moreover, they point to epigenetic silencing of the expression of existing genetic variants and the suppression of transposon-induced new genetic variation as two major mechanisms underlying piRNA pathway-mediated canalization.     

高层建筑防火设计的几点探讨

由于高层建筑火灾隐患多,扑救难度大,因此,在建筑设计中应采取防火措施,以防火灾发生和减少火灾对生命财产的危害。建筑防火包括火灾前的预防和火灾时的措施两个方面,前者主要为确定耐火等级和耐火构造,控制可燃物数量及分隔易起火部位等;后者主要为进行防火分区,设置疏散设施及排烟、灭火设备等。针对高层建筑发生火灾的特点,国内外在防火设计上达成了共识,本文做出详细分析。     

浅谈零星工程建设管理

针对零星工程建设管理,文章从零星工程建设管理的必要性、零星工程建设管理的控制点及零星工程建设管理流程三个方面进行了阐述,并总结了零星工程建设管理的流程图。     

New Challenge of Global Change

We briefly review the development of global change research program in the last decade, introduce the new structure of global change in the next decade, discusses the opportunities and challenges for China, and gives some suggestion on the supporting fields during the Tenth Five-Year Plan Period from 2001 to 2005.     

PIXE and INAA studies on ancient potteries from Guangfulin relics in Shanghai

This paper reports the study of ancient potteries unearthed from the Guangfulin site in Shanghai. From the external features of these potteries, archeologists can classify them into two different cultural types: Liangzhu culture (local culture) and a new culture which might be derived elsewhere, but they can not recognize whether they were produced in the same place. Proton induced X-ray emission (PIXE) and instrumental neutron activation analysis (INAA) were used to measure the chemical compositions of the samples and the results were analyzed using multivariate statistics. We found that the two methods can complement each other and their database can be mutually referenced in the study of ancient potteries. Experimental results show that the compositions of the potteries from the two cultural types are different, which means that the materials for ancient potteries of new culture originate from elsewhere. This supports the archaeologists’ speculation that a group of ancient people migrated to Shanghai from some other places 4000 years ago.     

Synthesis and Spectrum Characteristic of New Organic Fluorescence Dyes of Four Pyrazoline Compounds

1 Introduction Pyrazoline derivatives are formed by benzeno-hydrazone cyclization.Because cyclization leads to double bond hindering ,the compounds show stronger fluorescence .The pyrazoline has N atom which attends conjugation by donating electron,and it has higher hole-transport efficiency and some photoelectron characteristics,so it has been widely used as hole-transport materials in the electrophotography and Electroluminescence fields~([1-4]). Pyrazoline derivatives are not only excellen…