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51.
用显微注射方法,将精子注入小鼠卵母细胞卵周隙中,使精卵体外受精获得受精卵。并对精子注射后卵母细胞的成活率、受精率和受精卵的发育率以及移植后受胎率等方面进行了系统的研究,结果表明:卵子存活率为75.36%(1309/1737),其中264枚卵裂,受精率为20·17%(264/1309),卵裂卵经体外培养后有192枚发育到桑椹胚及胚泡期,发育率为72.73%(192/264)。将其中73枚(桑椹胚10枚,肛泡63枚)胚胎移植到10只假孕受体子宫中,一只受体妊娠产仔9只,仔鼠发育正常。 相似文献
52.
Aberrant DNA methylation patterns in cultured mouse embryos 总被引:1,自引:0,他引:1
Mouse early embryos undergo genome-wide demethylation and remethylation events during pre-implantation development. Abnormal methylation reprogramming is thought to be associated with development arrest. Using immunofluorescence staining with an antibody against 5-methylcytosine (MeC), we examined the genome methylation patterns of mouse embryos cultured in vitro. The results did not show the difference in staining patterns between development-blocked two-cell embryos that cultured in vitro and the two-cell embryos that were freshly collected from the donor mice. But in vitro-arrested morulae displayed a strong positive staining when compared to the morulae freshly collected from the donor mice. At the blastocyst stage, although most embryos showed the expected methylation patterns, with highly stained inner cell mass (ICM) and weekly stained trophectoderm (TE), a proportion of embryos were dimly stained in both ICM and TE. These results indicated that the methylation profile of the embryos could be changed by culturing in vitro when the embryos were in the transition from morulae to blastocyst. 相似文献
53.
Summary The dibutyryl analog of cCMP enlarged the nuclei of trophoblast gian cells and promoted blastocyst development. The result suggests that cCMP has a trophic effect on embryonic development, specifically by altering the size of the trophoblast cell nucleus but does not enhance trophoblast cell proliferation processes. 相似文献
54.
Summary Hexose transport in Swiss 3T3 cells was increased by treatment with dichloroacetic acid as well as by treatment with insulin. Neither extra-nor intracellular Ca2+ was found to be involved in their stimulatory action. On the other hand, the removal of intracellular Mg2+ resulted in a loss of the stimulation. These results suggest that dichloroacetic acid stimulates the hexose transport in Mg2+-dependent manner, similar to that of insulin.This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, and the Ministry of Health and Welfare of Japan. 相似文献
55.
56.
以染色体断裂剂:丝裂霉素C和环磷酸胺以及纺锤体损伤剂:长春新碱和秋水仙碱四种受试物分别进行小鼠骨髓微核试验.实验结果表明,两类诱变剂所诱发的大微核形成率之‘间有非常显著的差别(P<0.01),显示了微核形成的不同机理.本方法对快速鉴别染色体断裂剂和纺锤体损伤剂具有一定的应用价值. 相似文献
57.
Summary Ectopic pituitary transplantation induced a high incidence of adenomyosis in SHN mice. Early signs of the development of adenomyosis were the penetration of stromal connective tissue into myometrium followed by uterine gland invasion. Associated with these changes, the inner layer of myometrium showed the involution of smooth muscle cells and distended intercellular spacesThis work was supported in part by Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan 相似文献
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59.
Zh. A. Medvedev M. N. Medvedeva H. M. Crowne 《Cellular and molecular life sciences : CMLS》1984,40(11):1282-1284
Summary Electrophoretic analysis of histones and non-histone acid-soluble proteins in active (nuclease sensitive) and inactive chromatin from liver of young and old CBA mice and in age-related hepatocarcinomas showed a higher ratio of NHP: histones in active chromatin in old cells. Some liver- and hepatoma-specific fractions of non-histone proteins have been identified as chromatin matrix proteins. 相似文献
60.
A. Dobozy L. Kemény M. Csató J. Hunyadi A. Sz. Kenderessy N. Simon 《Cellular and molecular life sciences : CMLS》1984,40(12):1403-1404
Summary An enhancement effect of mouse erythrocyte rosette forming (MERF) cells on the production of migration inhibitory factor, chemotactic factor for neutrophils and skin reactive factor in T-lymphocyte cultures stimulated with the purified protein derivative of tuberculin was observed. We consider it likely that the MERF cells, possessing the appropriate cell surface constituents to construct an immunogenic moiety, present antigen on their surfaces to elicit lymphokine production.This work was supported by the Scientific Research Council, Ministry of Health, Hungary, Code No. 421030401 1/s 相似文献