基于GIS的生态功能区划在生态城市建设中的应用——以陕西省凤县为例

在对目前生态功能区划众多方法进行比较的基础上,将GIS技术应用到陕西省宝鸡市凤县生态功能区划中。根据研究区生态环境要素、生态环境敏感性及生态服务功能的差异,把区域划分成四个不同的生态功能区,即北部森林生态重点保护区,中部林、药、养殖、矿产限制开发区,中部粮、椒、果、城镇与贸易重点开发区,南部生物多样性保护与生态旅游区,并综合考虑区域实际情况进行深一步规划研究。     

Specific anti-tumor effect induced by attenuated Salmonella typhimurium vaccine expressing extracellular region of vascular endothelial growth factor receptor 2

The purposes of this research were to study the stable expression of exogenous gene encoding therapeutic protein in attenuated Salmonella typhimurium, observe the metabolism of oral gene vaccine carried by attenuated Salmonella typhimurium in BALB/c mouse, and investigate the feasibility of prevention and treatment of tumors by the recombinant bacteria. Recombinant plasmid pcDNA3.1 VEGFR2(n1-7) was transformed into competent attenuated Salmonella typhimurium SL3261 to develop oral DNA vaccine SL3261-pcDNA3.1 VEGFR2(n1-7). To observe whether the exogenous gene can be expressed in the recombinant bacteria, PCR was performed to amplify the CMV promoter of the eukaryotic expression vector as the proof of stable expression of exogenous protein; transmission elec- tron microscopy (TEM) was applied to observe the morphology of the recombinant bacteria to confirm that the exogenous gene has no impact on the growth of the bacteria, and then BALB/c mice were immunized with the gene vaccine. After inoculation of the gene vaccine, the recombinant bacteria SL3261 could be detected in the tissues such as small intestine, colon, liver and spleen. And then, mice in each group were challenged with tumor cells. The results of animal experiment showed that tumor growth of the mice in experimental group was inhibited and survival time of immunized mice was prolonged compared with control groups. A higher lymphocyte infiltration in tumors from animals treated with DNA vaccine was observed. Immunohistochemical analysis of tumor samples revealed an en- hanced accumulation of CD8 cytotoxic T lymphocytes, as well as an increase in CD4 cells in the tumors of animals treated with the oral gene vaccine compared to tumors from control group mice. Ultrastructure of the tumor tissue showed that tumor cells in the samples of the immunized mice were well-differentiated. Our research confirmed that the exogenous gene can be stably expressed in the attenuated Salmonella typhimurium and has no impact on the growth of the recombinant bacteria; the exogenous gene can de delivered to the host by attenuated Salmonella typhimurium to produce anti-tumor effect with no obvious cytotoxity to the host. In this study, it is established that attenuated Salmonella typhimurium could be used as a vector for oral gene vaccine, and our study provided a theoretical basis for the body distribution and the metabolism of the recombinant bacteria. This strategy may provide a simple, safe and effective way for the prevention and treatment of tumors.     

云南数字台网谱震级的测定和地震辐射能的计算

运用Duda等提出的谱震级测定公式,结合云南区域数字台网宽频带记录资料测定了发生在网内的中强震(4.0相似文献   

Solving Non-Uniqueness in Agglomerative Hierarchical Clustering Using Multidendrograms

In agglomerative hierarchical clustering, pair-group methods suffer from a problem of non-uniqueness when two or more distances between different clusters coincide during the amalgamation process. The traditional approach for solving this drawback has been to take any arbitrary criterion in order to break ties between distances, which results in different hierarchical classifications depending on the criterion followed. In this article we propose a variable-group algorithm that consists in grouping more than two clusters at the same time when ties occur. We give a tree representation for the results of the algorithm, which we call a multidendrogram, as well as a generalization of the Lance andWilliams’ formula which enables the implementation of the algorithm in a recursive way. The authors thank A. Arenas for discussion and helpful comments. This work was partially supported by DGES of the Spanish Government Project No. FIS2006–13321–C02–02 and by a grant of Universitat Rovira i Virgili.     

一类3-紧优双环网无限族

到给定顶点数的双环网的最小直径是图论和计算机工作者广泛研究的问题。虽然大多数双环网可以达到紧优,但仍然有一部分双环网不能达到。本文给出了一类3-紧优的双环网无限族。     

电动势的测定及其应注意的问题

本课题通过用不同的方法测定的电动势分析,经过电动势测定的误差分析,找到各种方法的利弊,并且对电极制备以及测定电动势的装置进行改进,近而对问题进行探讨。改进后找到实验和研究的捷径,从而在高等学校化学实验以及生产中节省很多的人力和财力。     

一类分布参数混合系统的稳定性分析

将混合系统推广到分布参数环境下,提出分布参数混合系统的概念.基于混合系统和分布参数系统的建模方法,建立了一类分布参数混合系统的模型,并对其稳定性进行分析.运用算子半群理论以及混合系统的五元组描述法及其稳定性关系比较理论的结论,通过选择适当的映射,将分布参数混合系统的的稳定性问题转化为集中参数下混合系统稳定性问题,进而得出了一致渐近稳定的充分条件并加以证明.仿真实例验证了文中结论的正确性.     

一类时滞系统的模糊预测控制方法

对模糊控制和预测控制相结合的控制方法进行研究,提出了一种简单易于实现的模糊预测控制的组合方法.此方法将模糊控制的定性分析特点和预测控制的定量分析特点有效地结合起来,同时积分器的引入可以消除稳态偏差.仿真结果表明,该方法对控制作用的滞后时间不确定的系统十分有效.     

基于单片机的煤气渗漏监控系统的设计

以8051单片机为核心，结合防爆型煤气浓度监测传感器及AD0832等，实现煤气化生产各环节中煤气渗漏的实时监测、采集、处理.     

电子商务的应用及对企业的影响

简述了电子商务理论的演变过程及电子商务的应用形式,包括电子数据交换、网上营销、网络银行等。探讨了电子商务的应用使企业面临的历史变革,指出作为21世纪的一种基本商务形式,电子商务已经成为具有敏锐战略眼光企业家的选择。