抗体药物的植物反应器生产

大量的重组抗体被用于疾病的预防、诊断和治疗.目前,这些重组抗体绝大多数是利用鼠源细胞生产的,它的缺点是有潜在的隐患和价格昂贵.然而,植物作为反应器生产抗体药物既具有安全性又可大规模廉价生产的特点,具有广阔的前景.     

Production of transgenic mice carrying green fluorescence proteingene by a lentiviral vector-mediated approach

A pseudo-lentivirus, which carries green fluorescence protein (GFP) expressing cassette, was injected into the perivitelline space of murine fertilized oocytes before transplanting into the oviducts of the foster mothers. The GFP transgenic pups were then obtained. By PCR amplification, fluorescent microscopy and flow assisted cytometry sorting analysis, we found that the integration rate of the transgene was estimated at above 40%. Real-time PCR analysis indicated that the copy number of the integrated GFP cassette was around 40. Fluorescent in situ hybridization analysis demonstrated that the integration pattern was random but inheritable. The transgenic mice with multi-integration sites and various expression levels possessed a great value in practice as well as research. The approach reported herein provides an efficient way to generate and screen the transgenic mouse strains.