CODCr测定中氯离子的干扰及消除方法研究

通过实验研究氯离子对CODCr测定的干扰程度，采用硫酸汞掩蔽法，氯离子扣除法．稀释倍数法等方法去除氯离子的干扰，最终确定出处理的最佳方案是在不掩蔽氯离子条件下，对水样中氯离子产生的CODCr值进行扣除、且实验证明此种方法对实际水样同样适用。方法适用氯离子范围为0～8000mg/L。     

基于自适应邻域的空域错误掩盖算法

针对以固定邻域内的像素为参考进行图像错误掩盖时,往往容易导致边界信息的丢失或产生阴影和虚假条纹的问题,提出了根据错误块邻域图像特征选择自适应参考像素集的空域错误掩盖算法.介绍了一种有效的分割方法,将邻域分割为与错误块相关和无关的两个区域,并以相关区域内的像素为参考进行空域内插.实验结果表明,该算法掩盖的视频图像既能有效地重建多个方向的边界信息,又能抑制或消除无关区域的影响,可广泛应用于掩盖MPEG、JPEG和H.26x等基于块编码码流中的错误.     

基于盲源分离的一种快速独立分量分析算法

基于盲源分离理论提出了一种快速独立分量分析 (FastICA)算法 .该算法以负熵作为独立性判决准则 ,在迭代过程中引入遗忘因子 ,加快了算法的收敛速度 .仿真结果表明 ,该方法能快速、有效地分离混迭信号     

DS-CDMA中空时多用户检测算法及其性能分析

阐述了空时多用户检测的现状，介绍和分析了空时线性多用户检测算法，盲空时多用户检测等几种主要的空时多用户检测技术，并对几种空时多用户检测方法进行了误码率仿真分析，验证了空时多用户检测可以大大改善DS-CDMA系统的性能。     

Symmetrical directional cloning: An efficient method to prepare hairpin RNA interference constructs

RNA interference (RNAi) is a loss-of-function approach by which double-stranded RNA (dsRNA) initiates degradation of homologous mRNAs in a sequence specific manner. The dsRNA molecules can be produced in vitro or in vivo, and can be introduced to cells in a number of ways. Here we report a more efficient method for the cloning of inverted repeat DNA fragments into expression vectors that can be transcribed into effective dsRNA molecules in vivo or in vitro. This method, named Symmetrical Directional Cloning (SDC), takes the advantage of compatible non-palindromic restriction enezyme sites, which allow one to directionally clone a single PCR product in both the sense and antisense orientations together into a vector. SDC allows for the directional cloning of inverted repeats using a single PCR product; it requires only one cut site on each side of the loop. Hence this method is more cost effective and less time-consuming. At least 21 commercially available restriction endonucleases can be used as cloning sites for the SDC method. The efficacy of dsRNA expression vectors prepared by SDC has been demonstrated by targeting a negative regulator of the signaling pathway mediating the response of cells to phytohormone, gibberellins (GA), in the aleurone cells.     

RNA interference-mediated inhibition of Hepatitis B Virus replication

Persistent and recurrent infection of hepatitis B virus (HBV) represents one of the most common and severe viral infections of humans, and has caused a formidable health problem in the affected countries. Currently used antiviral drugs have a very limited success on controlling HBV replication and infection. RNA interference (RNAi), a process by which double-stranded RNA (dsRNA) directs sequence-specific degradation of target mRNA in mammalian and plant cells, has recently been used to knockdown gene expression in various species. In this study, we sought to determine whether RNAi-mediated silencing of HBV viral gene expression could lead to the effective inhibition of HBV replication. We first developed RNAi vectors that expressed small interfering RNA (siRNA) and targeted the HBV core or surface gene sequence. Our results demonstrated that these specific siRNAs efficiently reduced the levels of corresponding viral RNAs and proteins, and thus suppressed viral replication. Treatment with siRNA gave the greatest reduction in the levels of HBsAg (92%) and in HBeAg (85%) respectively in the cultured cell medium. Our findings further demonstrated that the RNAi-mediated antiviral effect was sequence-specific and dose-dependent. Therefore, our findings strongly suggest that RNAi-mediated silencing of HBV viral genes could effectively inhibit the replication of HBV, hence RNAi-based strategy should be further explored as a more efficacious antiviral therapy of HBV infection.     

自适应数字波束空域相干干扰抑制方法

在干扰相干背景下,接收信号协方差矩阵中信号空间的一些向量收敛到噪声空间中,仅利用二阶信息不能准确地得到干扰的方向信息,因而干扰抑制效果较差。为此,提出了一种自适应数字波束空域相干扰抑制方法。该方法是首先基于高阶累积量估计出广义方向矢量,然后根据最优均方逼近原则,结合切比雪夫加权系数形成波束,来抑制相干干扰。仿真结果表明,所提方法能够有效地抑制相干干扰。     

基于数据矩阵分解的相干源方向估计新方法

提出一种相干源存在情况下的波达方向估计新算法。利用阵列快拍数据构造数据矩阵,相关去噪后,通过奇异值分解获得低维信号子空间的估计,然后运用ESPRIT思想估计出波达方向。无论是否存在相干源,新算法均能有效估计出波达方向,并且无需角度搜索,运算量小。该算法虽基于一维线阵,但可直接推广到具有线性性质的二维平面阵,如L形阵、十字阵、双平行线阵等。计算机仿真验证了算法的有效性     

调频线性度对线性调频信号性能影响分析

针对去调频和脉冲压缩两种不同的处理方式,深入研究了线性调频信号调频线性度对信号性能的影响,得到LFM信号带宽越大,误差频率越低,对线性度要求越高的结论,且在相同边带抑制的条件下,去调频处理比脉冲压缩处理对LFM信号线性度的要求大约提高了一倍。仿真结果验证了理论分析的正确性。     

透明转发器卫星通信系统在干扰条件下的互调分量

研究了有干扰时透明转发器卫星通信系统中的互调分量。首先，对有干扰时硬限幅条件下窄带系统的互调分量和DS扩频通信系统中的相关互调分量进行了理论分析；然后，针对实际系统，建立了系统仿真模型，进行了计算机性能仿真，通过对仿真结果的分析，得到一些结论。研究成果对了解透明转发器卫星通信系统在强干扰条件下存在的互调分量的影响及其量化结果，从而设计具有针对性的卫星通信抗干扰系统具有实际意义。