岩体开挖结构面特性分析的边界元法

在力学计算中如何很好地模拟岩体结构面破裂一直是一个难题.通过对结构面特性的分析,利用边界元法给出了结构面模拟的两种模型.模型将结构面分别假想为刚性连接和弹性连接,在结构面达到强度极限之前,结构面处于平衡状态,当其达到强度极限时,连接断裂,这样就避免了寻找结构面合理参数的工作,为更好地模拟结构面岩体的破坏提供了全新的方法.     

榆次西窑水源地三叠系刘家沟组裂隙水分析

根据西窑水源地的自然地理与地质构造，分析与论证了西窑水源地三叠系刘家沟组裂隙水的成因。并对刘家沟组裂隙水储量进行了预测。     

取料机导轮组长轴断裂原因分析及对策

针对取料机导轮组长轴断裂的原因，采取了相应的改进措施，避免了长轴的断裂，取得了良好的效果。     

断裂力学中应力强度因子的2种解法

提出Fourier积分变换和有限元数值解法求解工程断裂力学中应力强度因子KⅠ的2种解法，而KⅡ可通过两者之间的关系导出，其解值与实际均基本吻合；初步分析和验证了后者解析解的误差关系。这2种方法具有简单方便的特点。     

"T"型外固定架治疗青壮年严重移位的肱骨外科颈骨折

目的：探索青壮年严重移位肱骨外科颈骨折的手术入路及固定技术，方法：取肩关节前侧入路的下部分，不离断三角肌附丽，不剥离骨折周围软组织，“T”型外固定架固定术后3～5d开始肩关节主动功能练习，结果：所有32例手术患者无一例感染，均在6周左右取得了良好的骨性愈合，肩关节功能评定按JOA标准，优良率占96％，结论：应用“T”型外固定架治疗青壮年严重移位肱骨外科颈骨折，切口小，组织损伤少，操作简单，固定可靠，利于关节早期活动，恢复肩关节功能，是治疗青壮年严重移位肱骨外科颈骨折的一种好方法。     

DS-CDMA中空时多用户检测算法及其性能分析

阐述了空时多用户检测的现状，介绍和分析了空时线性多用户检测算法，盲空时多用户检测等几种主要的空时多用户检测技术，并对几种空时多用户检测方法进行了误码率仿真分析，验证了空时多用户检测可以大大改善DS-CDMA系统的性能。     

固溶处理对镍-铬-钼合金力学性能的影响及断口分析

采用金相显微镜、X射线衍射和扫描电镜等方法研究了热压成形镍-铬-钼合金固溶前后的金相组织、相的组成、断口形貌,以及固溶处理对合金力学性能的影响.研究结果表明:经1250 ℃/4 h固溶处理后的合金全为Ni的固溶体,而未经固溶处理和经1200 ℃/4 h固溶处理的合金除了含有Ni的固溶体外,还有单质Mo和一些金属间化合物;未经固溶处理的合金呈脆性断裂,拉伸强度要比固溶后合金的强度低,而固溶后合金呈延性断裂.经 1250℃/4 h和1200 ℃/4 h固溶处理后合金显示出以韧窝为主的断口形貌,与合金的延性断裂力学性能测试结果一致,而未经固溶处理的合金呈现以解理为主的断口形貌,与合金的脆性断裂力学性能测试结果一致.固溶处理前合金的硬度要比处理后的合金硬度高.     

Symmetrical directional cloning: An efficient method to prepare hairpin RNA interference constructs

RNA interference (RNAi) is a loss-of-function approach by which double-stranded RNA (dsRNA) initiates degradation of homologous mRNAs in a sequence specific manner. The dsRNA molecules can be produced in vitro or in vivo, and can be introduced to cells in a number of ways. Here we report a more efficient method for the cloning of inverted repeat DNA fragments into expression vectors that can be transcribed into effective dsRNA molecules in vivo or in vitro. This method, named Symmetrical Directional Cloning (SDC), takes the advantage of compatible non-palindromic restriction enezyme sites, which allow one to directionally clone a single PCR product in both the sense and antisense orientations together into a vector. SDC allows for the directional cloning of inverted repeats using a single PCR product; it requires only one cut site on each side of the loop. Hence this method is more cost effective and less time-consuming. At least 21 commercially available restriction endonucleases can be used as cloning sites for the SDC method. The efficacy of dsRNA expression vectors prepared by SDC has been demonstrated by targeting a negative regulator of the signaling pathway mediating the response of cells to phytohormone, gibberellins (GA), in the aleurone cells.     

RNA interference-mediated inhibition of Hepatitis B Virus replication

Persistent and recurrent infection of hepatitis B virus (HBV) represents one of the most common and severe viral infections of humans, and has caused a formidable health problem in the affected countries. Currently used antiviral drugs have a very limited success on controlling HBV replication and infection. RNA interference (RNAi), a process by which double-stranded RNA (dsRNA) directs sequence-specific degradation of target mRNA in mammalian and plant cells, has recently been used to knockdown gene expression in various species. In this study, we sought to determine whether RNAi-mediated silencing of HBV viral gene expression could lead to the effective inhibition of HBV replication. We first developed RNAi vectors that expressed small interfering RNA (siRNA) and targeted the HBV core or surface gene sequence. Our results demonstrated that these specific siRNAs efficiently reduced the levels of corresponding viral RNAs and proteins, and thus suppressed viral replication. Treatment with siRNA gave the greatest reduction in the levels of HBsAg (92%) and in HBeAg (85%) respectively in the cultured cell medium. Our findings further demonstrated that the RNAi-mediated antiviral effect was sequence-specific and dose-dependent. Therefore, our findings strongly suggest that RNAi-mediated silencing of HBV viral genes could effectively inhibit the replication of HBV, hence RNAi-based strategy should be further explored as a more efficacious antiviral therapy of HBV infection.     

自适应数字波束空域相干干扰抑制方法

在干扰相干背景下,接收信号协方差矩阵中信号空间的一些向量收敛到噪声空间中,仅利用二阶信息不能准确地得到干扰的方向信息,因而干扰抑制效果较差。为此,提出了一种自适应数字波束空域相干扰抑制方法。该方法是首先基于高阶累积量估计出广义方向矢量,然后根据最优均方逼近原则,结合切比雪夫加权系数形成波束,来抑制相干干扰。仿真结果表明,所提方法能够有效地抑制相干干扰。