图书馆管理手段的探讨

论述了图书馆管理中期刊装订的必要性以及期刊流通的必然性,分析了期刊装订与期刊流通的辨证关系,确定了期刊装订与期刊流通在期刊工作中的重要地位.     

实时荧光定量PCR检测原发性肝癌中hNTKL-BP1基因的表达

分别提取手术切除人原发性HCC肝癌和癌旁肝组织总RNA并逆转录为cDNA,在hNTKL-BP1基因的高保守区设计相应引物和Taqman荧光探针,实时检测PCR产物的荧光强度,采用2-△△Ct方法分析比较该基因在人类肝癌组织和癌旁肝组织中的表达差异.结果是44例原发性HCC肝癌组织中hNTKL-BP1基因表达水平显著高于癌旁肝组织(t=2.69,P<0.01),肝癌组织与癌旁肝组织hNTKL-BP1基因表达差异和肿瘤大小有一定的相关性(P=0.028),hNTKL-BP1基因可能与肝癌的发生、发展有一定相关性.     

Differential binding of conA and WGA on the cell surface,the role of sialic acid in their expression and the increased activity of sialidase after cis-Platin treatment

Summary It is reported that concanavalin A (conA) and wheat germ agglutinin (WGA) have a differential binding pattern on normal mouse spleen lymphocytes and the surface of Dalton's lymphoma cells. It is suggested that sialic acid on the cell surface controls the expression of lectin binding sites. Further, it has been observed that the increased release of sialic acid from cell surfaces aftercis-dichlorodiammine platinum (II) (cis-Platin) treatment is due to the increased activity of sialidase.To whom reprint requests should be addressed.     

COM/CORBA互操作中的对象标识及绑定研究

随着ＣＯＭ和ＣＯＲＢＡ的迅速推广使用，用户迫切需要ＣＯＭ／ＣＯＲＢＡ互操作系统以实现ＣＯＭ和ＣＯＲＢＡ应用的集成，因此很多ＣＯＲＢＡ产品都遵照ＯＭＧ组织的ＣＯＭ／ＣＯＲＢＡ互操作规范提供了互操作的功能。然而规范中的对象标识及联编机制还存在问题，导致应用程序无法在不同的互操作系统上移植，而且在通用映射实现方式中，无法按照规范中定义的机制实现ＣＯＲＢＡ客户对ＣＯＭ对象的联编。文中提出了一种统一的对象标     

无侧移刚架内力计算的通用迭代法

介绍了一种通用迭代法,可用来求解各种无侧移刚架内力及杆端弯矩.     

The short C-terminal hydrophilic domain of NhaH Na~ /H~ antiporter from Halobacillus dabanensis with roles in resistance to salt and in pH sensing

The Na /H antiporter plays key roles in maintaining low cytoplasmic Na level and pH homeostasis,while little is known about the Carboxyl-terminal hydrophilic tails of prokaryotic antiporters.In our previous study,the first Na /H antiporter gene nhaH from moderate halophiles was cloned from Halo-bacillus dabanensis D-8 by functional complementation.A topological model suggested that only nine amino acid residues(395PLIKKLGMI403) existed in the hydrophilic C-terminal domain of NhaH.The C-terminal truncated mutant of NhaH was constructed by PCR strategy and designated as nhaH△C.Salt tolerance experiment demonstrated that the deletion of hydrophilic C-terminal nine amino acid resi-dues significantly inhibited the complementation ability of E.coli KNabc,in which three main Na /H antiporters nhaA,nhaB and chaA were deleted.Everted membrane vesicles prepared from E.coli KNabc/nhaH△C decreased both Na /H and Li /H exchange activities of NhaH,and also resulted in an acidic shift of its pH profile for Na ,indicating a critical role of the short C-terminal domain of NhaH antiporter in alkali cation binding/translocation and pH sensing.     

人类基因组中L1元件及其5＇UTR的研究

讨论了L1在人类染色体中的分布密度与染色体长度以及L1的密度与基因密度之间的关系．发现在大多数染色体上L1的密度和染色体的长度表现为正相关，而L1的密度和染色体中基因的密度表现为负相关；对人类L1的5’UTR进行了详细的研究后，发现在L1元件的5’UTR部分含有许多转录因子结合位点．对基因的转录起始位点上游进行统计分析，发现其中含有大量的L1序列．通过对人类的EST数据库进行BLAST分析，新发现了51条和人类L15’UTR相似性较高的EST片断，根据它们分布的组织特异性，说明L1元件可能调控与发育过程有关的基因的表达；最后应用多样性增量的方法对人类和大鼠的L1 5＇UTR进行了区分，得到了较好的预测结果，说明大部分L1的5’UTR的调控作用是人类特有的．     

量子阱中界面声子和体声子对束缚极化子结合能的影响(英)

用变分法研究半导体量子阱中束缚极化子结合能，在计算中包括了界面声子和体声子的影响给出了束缚极化子基态能量和结合能随阱宽变化的数值结果．研究发现，界面声子和体声子对结合能的贡献分别在窄阶和宽阱情况下起主导作用，总结合能随阱宽的增大而减小