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51.
Rice (Oryza sativa L.) eating quality is one of themost important traits. Amylose content (AC) in rice en-dosperm is a major index affecting rice eating quality[1,2].It has a negative correlation with gel consistency of rice[3].Based on amylose content, r… 相似文献
52.
Effects of CDK4 antisense RNA on Human breast cancer cell proliferation and expression of cyclinD1, cyclinE and CDK2 总被引:1,自引:0,他引:1
The role of CDK4 in human breast cancer cell proliferation and expression of cyclinD1, cyclinE and CDK2 has been investigated
using inhibition of CDK4 expression by antisense RNA. When CDK4 expression was inhibited, the rate of cell proliferation and
tumorigenecity decreased apparently. This indicates that CDK4 plays an important role in formation and development of breast
tumor. The results of Northern blot analysis showed that the levels of cyclinDl and CDK2 mRNAs changed slightly whereas the
level of cyclinE mRNA decreased obviously. It is suggested that the expression of CDK4 is necessary for imction of cyclinE
expression. Thus, inhibition of CDK4 expression affects not only the role of CDK4 itself but also the role of other genes. 相似文献
53.
反义RNA的存在最先是在质粒和其它原核附属DNA元件中被证实的.对这些体系深入的研究揭示了原核反义RNA结构和功能的联系,以及正义/反义RNA相互作用的机理.本文着重叙述反义RNA在质粒复制中的作用及其进展. 相似文献
54.
利用CaCl2法成功地将寡核苷酸导入大肠杆菌JM109细胞。质粒pBR322转化结果表明,寡革酸片段5’-AGCGGGAATAAGGGAA-3’在转化前(或后)与靶序列结合均能抑制β-内酰胺酶基因的表达,细菌生长受到抑制。体外聚丙烯酰胺凝胶电泳结果表明,该片段能与靶序列形成三链结构。这些结果表明多聚嘌呤寡核苷酸是通过与靶序列形成三链DNA来抑制β-内酰胺酶基因的表达。 相似文献
55.
介绍了反义RNA的概念及作用机理,对反义RNA技术及其在农业领域的应用进行了概述.反义RNA技术是借助基因重组技术,根据碱基互补原理,用人工合成或生物体合成的特定RNA片段(或其化学修饰物)抑制或封闭基因表达的技术。在农业领域反义RNA技术主要应用于果实性状及品质的调控,对观赏植物性状的调控,植物抗病,控制油料种子中脂肪酸的合成,控制雄性不育,降低或去除食物中有害化学成分等方面其功能获得可靠的成效. 相似文献
56.
Honghong Zheng Lijia Qu Meihua Liu Yi Zhang Yunping Shen Junming Wei Naisui Pan Hongya Gu Zhangliang Chen 《科学通报(英文版)》2000,45(21):1921-1926
It was shown in a previous analysis that D5 gene from rice (Oryza sativa L.) was an anther-specific gene encoding a chalcone synthase-related protein. In this study, D5 gene was found specifically expressed in tapetum cells as well as in the peripheral cells of the vascular bundle of rice anthers by RNA in situ hybridization. In order to study its function, D5 was transformed into rice in both sense and antisense directions driven by a rice Actin 1 promoter. It has been observed that the pollen grains from the antisense D5 transgenic rice plants are abnormal, indicating that D5 plays a critical role in rice pollen development. 相似文献
57.
To investigate the biological roles of STAT3 in the regulation of growth and differentiation of leukemia cells, we modified a murine myeloid leukemia cell line Ml with STAT3 antisense RNA. The effects of STAT3 antisense RNA on the growth arrest and terminal differentiation of Ml cells induced by interleukin 6 (IL-6) were determined. It was found that STAT3 antisense RNA blocked the activation of STAT3, and reduced the growth arrest and terminal differentiation of IL-6-induced Ml leukemia cells. These results indicate that STAT3 activation is a necessary process for IL-6-induced growth arrest of Ml cells and for the differentiation of Ml cells into macrophage. 相似文献
58.
Antisense transcription: A critical look in both directions 总被引:3,自引:1,他引:2
59.
NING Lifeng LONG Zhitao HUANG Xiuqing SUN Lingling SANG Jianli 《科学通报(英文版)》2006,51(19):2335-2341
In mammalian cells, reversible phosphorylation of protein substrates is a major mechanism for many cel-lular processes. Protein kinases and protein phosphata- ses, which catalyze protein phosphorylation and dephosphorylation respectively, together with th… 相似文献
60.