贵阳市黔灵公园半野生猕猴日活动时间分配研究

本文报道了黔灵公园半野生猕猴种群日活动时间分配的研究结果。该种群日平均活动时间为７４０±３０分钟，日活动时间分配的结果是：取食占２１．１％，游走占２３．０％，休息占２８．０％等。还报道了该种群３个年龄组个体的时间分配差异性，及种群在四季变化中时间分配的差异性结果．最后讨论该种群内不同年龄组个体时间分配差异原因及种内不同种群间时间分配差异的原因。     

恒河猴犬瘟热病毒基因疫苗制备单克隆抗体的研究

从感染犬瘟热病毒死亡的恒河猴肝脏组织中提取总RNA,经RT-PCR扩增H全基因并克隆到真核表达质粒pVAX中,构建了基因疫苗pVAX-H。用制备的基因疫苗免疫BaLb/c小鼠,小鼠可以产生免疫应答,免疫鼠脾细胞与SP2/0骨髓瘤细胞融合后,经间接ELISA法筛选,获得5株稳定分泌抗CDV H蛋白的单克隆抗体的杂交瘤细胞株。该5株单抗对应不同的病毒抗原表位,且均不与CPV和MV发生反应, McAb诱生的腹水可体外中和犬瘟热病毒,其中2株中和效价大于1:256。结果表明,CDV H基因疫苗可制备具有一定中和效价的抗CDV的单克隆抗体。     

Induction of corneal epithelial progenitors from bone-marrow mesenchymal stem cells of rhesus monkeys in vitro

Bioengineered corneas are substitutes for human donor tissue that are designed to treat severe dis-ease affecting ocular surfaces. However,a shortage of candidate seed cells for bioengineering corneas is still a problem. Bone-marrow mesenchymal stem cells (MSCs) are capable of multilineage differen-tiation. Therefore,we determined whether MSCs differentiate into corneal epithelial cells (ECs). We applied three exoteric-microenvironmental systems to induce MSCs to become ECs. Induced MSC were identified by means of morphologic examination,immunocytochemical analysis,and flow cytometry. MSCs grown in one microenvironment had characteristics similar to those of corneal epithelial pro-genitors. Induced MSCs expressed markers for EC,including integrin β1,Cx43,Pax6,and P63. MSCs were successfully induced to become corneal epithelial progenitors. Therefore,the use of MSCs may hold substantial promise for reconstructing the ocular surface after corneal injury.     

太行山猕猴和大鼠中肝细胞之间的连接方式特例的扫描电镜观察

应用扫描电镜对太行山猕猴和Sprague-Dawley大鼠肝细胞表面进行了观察。发现在猕猴和大鼠的部分肝细胞表面都存在特征性的凹陷，该凹陷内分布有微毛，有的存在胆微管，同时，还发现细胞表面存在突出结构，该突出结构端部膨大，颈部稍细，由此认为这有可能是哺乳动物肝细胞表面的固有结构，端部膨大结构和表面凹陷极可能为一对特殊的肝细胞连接方式。     

川金丝猴的野外人工投食招引的方法

川金丝猴是一种树栖动物,机智敏捷,栖身于高山森林中,野外观赏和研究极为不便.为了能够野外近距离观赏和研究金丝猴,周至保护区与西北大学生命科学院合作,在野外招引川金丝猴取得成功.介绍了玉皇庙贡泥沟野外人工投食招引川金丝猴的方法,以便为今后研究和观赏川金丝猴提供帮助.     

黔金丝猴血清TEST与E2放射免疫测定

报道黔金丝猴血清睾酮（TEST）与血清雌二醇（E2）放射免疫测定。采用贵州梵净山自然保护区管理局野生动物救护中心饲养3年的5只黔金丝猴，取下肢静脉血，采用中美合资九鼎医学生物工程有限公司生产的血清睾酮免疫测定试剂盒、血清雌二醇免疫测定试剂盒，对黔金丝猴及藏酋猴分别进行体外双抗体放射免疫测定（RIA）。结合野生动物救护中心野外调查资料和饲养资料，分析其TEST和E2浓度与龄阶、性别、在种群中所处等级、和发育状况的关系。     

笼养白头叶猴的食物选择和食物量的研究

研究了笼养条件下白头叶猴觅食的行为序，雌雄个体对食物的选择，夏季和冬季的日食量，笼养白头叶猴的觅食行为序由探究行为，抢食行为，爬墙行为和进食行为等４步组成，除探究行为的持续时间在雌雄个体间有显性差异外，其他行为持续时间无显的性别差异，雌雄个体对食物的选择不同，包括对某一食物的取食先后顺序，取食时间和取食量不同，雄猴和雌猴的日食量夏季分别为（６５９．３±１３６．３）ｇ和（５９０．７±１０６．３）     

秦岭川金丝猴秋冬季节活动时间分配的初步研究

通过对秦岭川金丝猴活动时间分配的研究,探讨影响"家庭"活动时间分配的内在因素和环境因素.结果表明,在秦岭川金丝猴秋冬季活动时间的分配中,平均时间比例分别为:觅食占14.90±0.26%,移动占10.16±0.16%,理毛占5.68±0.63%,休息占69.26±0.24%.其时间分配从秋季到冬季有明显地变化,即休息和觅食时间增长,而理毛时间变短.对秦岭野生川金丝猴不同年龄组的日活动时间分配进行了比较,结果表明不同年龄组的活动时间分配是有差异的.     

一氧化氮合酶在猕猴中枢神经系统中的表达

研究神经元型一氧化氮合酶(NOS1)在灵长类动物中枢神经系统中的表达．方法：采用免疫组织化学技术，观察了一氧化氮合酶在正常猕猴脑和脊髓中的表达．结果表明NOS1阳性神经元分布于中枢神经系统的广泛区域，包括大脑皮质、海马、齿状回、尾壳核、纹状体、小脑皮质、脊髓前角、后角和中央灰质与中枢神经系统的诸多功能有关．     

Expression of Smad2 and Smad4 in rhesus monkey endometrium during the menstrual cycle and early pregnancy

Expression of Smad2 and Smad4 mRNAs in the endometrium of rhesus monkey on Days 8, 20 and 28 of the normal menstrual cycle and on Days 12, 18 and 26 of early pregnancy was detected using in situ hybridization. The results showed that Smad2 and Smad4 mRNAs were mainly localized in luminal epithelium and glandular epithelium. The expression of Smad2 mRNA in glandular epithelium was sustained at moderate level on Days 8, 20 and 28 of the menstrual cycle, while the expression of Smad4 gradually increased with the menstrual cycle. Both Smad2 and Smad4 mRNAs in functionalis glandular epithelium were expressed at the highest levels on Day 12 of early pregnancy, while in basalis glandular epithelium the most abundant expression of both Smads occurred on Days 12 and 18 of pregnancy. On Day 26, both Smads mRNAs were expressed at the lowest levels either in functionalis or in basalis. The data suggest that the epithelium is the major compartment where TGF-βs/activins exert their biological effects via Smads, and that Smad4 may play a role in the maintenance of endometrial gland function during secreting period of the menstrual cycle. During lacunar stage of early pregnancy, Smad2 and Smad4 are implicated in the tissue remodeling of endometrial functionalis and basalis, and during early villous stage both Smads are functional primarily in basalis.