群自同构全提升本盖结构

根据Ｆｒｙ的群与Ｇｒｕｅｎｂｅｒｇ的扩张范畴（Ｔｒ     

In vivo matrix-guided human mesenchymal stem cells

Microfracture of subchondral bone results in intrinsic repair of cartilage defects. Stem or progenitor cells from bone marrow have been proposed to be involved in this regenerative process. Here, we demonstrate for the first time that mesenchymal stem (MS) cells can in fact be recovered from matrix material saturated with cells from bone marrow after microfracture. This also introduces a new technique for MS cell isolation during arthroscopic treatment. MS cells were phenotyped using specific cell surface antibodies. Differentiation of the MS cells into the adipogenic, chondrogenic and osteogenic lineage could be demonstrated by cultivation of MS cells as a monolayer, as micromass bodies or mesenchymal microspheres. This study demonstrates that MS cells can be attracted to a cartilage defect by guidance of a collagenous matrix after perforating subchondral bone. Protocols for application of MS cells in restoration of cartilage tissue include an initial invasive biopsy to obtain the MS cells and time-wasting in vitro proliferation and possibly differentiation of the cells before implantation. The new technique already includes attraction of MS cells to sites of cartilage defects and therefore may overcome the necessity of in vitro proliferation and differentiation of MS cells prior to transplantation. Received 3 November 2005; received after revision 15 December 2005; accepted 4 January 2006     

小麦菌核性根腐病研究初报

小麦茵核性根腐病Sclerotium rolfsii Sacc,为半知菌亚门小核菌属,白绢病菌。从文献看,我国山东、江苏两省均有发生,但目前国内外研究不多。近年来,在冀东地区发病面积逐年上升,是目前生产上急需解决的问题。本文对该病的症状、病原、发病规律、分级标准和防治方法进行了初步的探讨。     

生命科学及其相关学科的研究(五)

简要介绍了当代生命科学及其相关学科的研究概况:包括异基因干细胞移植技术、基因疗法保护骨髓细胞、功能基因组学、细胞移植治疗心脏病、转基因抗虫水稻、用基因定位技术培育动物生产人体蛋白质、发现干细胞源以及高产优质抗病早熟棉的培育等研究进展及其效益。     

毛竹基腐病侵染循环的研究

报道毛竹基腐病侵染循环的研究结果：（１）病原菌以菌丝或孢子的形态在病竹，枯立竹和病株残体及土壤内潜伏越冬成为次年初次侵染的来源；（２）风、随风和雨和雨水反溅是孢子传播的主要途径；（３）在自然情况下，病菌主要通过幼嫩的无伤表皮侵入寄主体内；（４）通过田间观察和病变组织的分离培养试验结果，结合病菌本身的特性可以初步确定该病菌是通过分泌毒素杀死寄主细胞后进入寄主组织的；（５）该病没有再次侵染。     

Marrow mesenchymal stem cells transduced with TPO/FL genes as support for ex vivo expansion of hematopoietic stem/progenitor cells

A new marrow-derived mesenchymal stem cell (hMSC) line that could support expansion of hematopoietic stem/progenitor cells (HSPCs) was developed. Primary hMSCs were infected with retrovirus containing Flt-3 ligand and thrombopoietin genes. CD34+ cells from cord blood were expanded with primary hMSCs or transduced hMSCs. The expansion of total nucleated cells, CD34+ cells and mixed colonies containing erythroid and myeloid cells and megakaryocytes for 2 weeks coculture with transduced hMSCs was remarkably increased. The outputs of long-term culture-initiating cells for 2 and 4 weeks coculture with transduced hMSCs were also largely increased. The expansion rates of HSPCs with transduced hMSCs were unchanged for 6 weeks. In contrast, the expansion rates of HSPCs with primary hMSCs declined drastically through 6 weeks. SCID-repopulating cell expansion with transduced hMSCs for 4 weeks was significantly higher than that of uncultured CD34+ cells and HSPCs expanded with primary hMSCs. Received 21 June 2005; received after revision 30 July 2005; accepted 24 August 2005     

杂交茎瘤芥物质积累规律研究

对茎瘤芥杂交种的物质积累过程进行了研究,结果表明,茎瘤芥杂交种各器官的物质积累符合Logistic生长曲线;各器官旺盛生长期：茎为15．3-20．2叶龄,根为12．7-17．0叶龄,叶为12．6-16．8叶龄;茎瘤芥杂交种与常规种的物质积累规律有一定差异．     

井眼中钻柱稳定性分析的有限元法

通过引入单元荷载刚度矩阵,提出了一种用于分析钻柱结构稳定性问题的有限单元法。该方法可以用于分析变截面钻柱在变轴力和变横向力共同作用下的钻柱稳定性问题,也可用于分析弯曲井眼中钻柱的稳定性问题。在所建立的力学模型中,考虑了钻柱自重和井眼曲率的影响。分析结果表明,在斜直井眼中,除了钻柱自身密度及截面面积外,影响钻柱荷载的因素还有井眼状况及钻柱两端部的约束条件,但当钻柱长度超过８０ｍ时可以不考虑端部约束的影响。对于弯曲井眼,钻柱临界压力不仅与钻柱的长度、截面尺寸有关,还与井眼曲率有关,即随着曲率的增大而增大。     

法布里-珀罗半导体激光器的波长连续可调光脉冲生成技术研究（英文）

文章论述了基于注入锁定增益开关调制法布里-珀罗半导体激光器(FP-LD)的波长连续可调脉冲生产技术.包括两方面的内容:1自激注入锁定FP-LD系统以获得波长连续可调脉冲输出,在48 nm波长范围获得37 dB以上的边模抑制比(SMSR),重复频率为2.5 GHz;2带有波长选择反馈环的外注入锁定FP-LD系统以获得波长连续可调脉冲输出.在20.4 nm的波长调节范围内获得31 dB以上的边模抑制比(SMSR).系统结构简单,可工作在任意重复频率上.     

石杉碱甲对急性炎症小鼠室旁核和脑干c-Fos和GFAP表达的影响

目的:探讨石杉碱甲对急性炎症小鼠下丘脑室旁核和脑干神经元c-Fos和星形胶质细胞(astrocytes,AST)胶质原纤维酸性蛋白(glial fiberillany acidic protein,GFAP)表达的影响.方法:①采用蛋清致小鼠足趾肿胀模型,检测致炎后30min,1h,2h的小鼠足容积并计算肿胀度;②免疫组化技术检测孤束核、迷走背核及室旁核神经元c-Fos及星形胶质细胞GFAP的表达.结果:①与生理盐水组比较,不同浓度的石杉碱甲均可明显抑制蛋清致小鼠足肿胀(P0.05或P0.01).②与对照组相比,致炎组小鼠孤束核和室旁核内Fos免疫阳性神经元(Fos-immunoreactive neurons,Fos-IR)及GFAP免疫阳性星形胶质细胞(GFAP-immunoreactive astrocytes,GFAP-IR)数目均明显增多(P0.01),而迷走背核增加不显著(P0.05).与致炎组相比,石杉碱甲给药组孤束核、迷走背核及室旁核内Fos-IR神经元及GFAP-IR星形胶质细胞数目均明显增多(P0.01).结论:石杉碱甲对蛋清致小鼠足肿胀有明显的抗炎作用;石杉碱甲能够显著增强急性炎症小鼠孤束核、迷走背核及室旁核内神经元和星形胶质细胞的活动.