重组人硫氧还蛋白对大鼠内毒素肺损伤保护作用

目的:探讨重组人硫氧还蛋白1(rh Trx-1)对新生SD大鼠急性内毒素肺损伤的保护作用.方法:制备、鉴定rh Trx-1;将新生SD大鼠分为对照组、脂多糖(LPS)实验组和LPS+rh Trx-1干预组.腹腔注射LPS(质量分数为5 mg/kg),制备急性内毒素肺损伤模型,干预组于LPS注射前30 min腹腔注射rh Trx-1(质量分数为10 mg/kg),观察注射后24 h各组新生SD大鼠的一般情况以及死亡率;重复实验,每组分别于实验后的2、8 h随机处死大鼠,提取肺组织用于病理切片观察.结果:(1)成功制备rh Trx-1;(2)LPS组大鼠表现出活动少,反应差,精神萎靡,毛发无光泽,口周发绀等全身炎症反应症状,濒死状态时表现为毛色青灰,呼吸浅表;LPS+h Trx-1干预组动物症状明显减轻;阴性对照组、LPS实验组和LPS+rh Trx-1干预组大鼠24 h死亡率分别为0%,67%、17%(2=14.400,P=0.001);(3)LPS组肺组织损伤严重,可见肺组织结构不完整,肺泡间隔明显增宽,肺泡腔内有红细胞、炎症细胞及浆液渗出,血管周围组织可见白细胞浸润,毛细血管有明显充血及扩张表现,随时间延长有加重趋势,LPS+rh Trx-1干预组病理改变较内毒素组明显减轻,肺泡结构尚正常,轻度肺水肿,肺泡间隔稍增宽,红细胞渗出明显减少,肺泡及肺间质中白细胞浸润减少.结论:重组人硫氧还蛋白1对急性内毒素肺损伤的新生大鼠具有保护作用.     

科学级冷却型CCD—荧光图像系统定量性能的鉴定

以荧光探针Ｈｏｅｃｈｓｔ３３３４２（Ｈｏ３３３４２）特异性标记大白鼠精子和肝细胞ＤＮＡ，用科学冷却型ＣＣＤ显微荧光图像系统获取单个细胞的荧光图像，再以图像处理软件“Ｉｍａｇｅ－Ｐｒｏ ＰＬｕｓ”，分析测量各细胞的ＤＮＡ相对含量和细胞核的相对面积。实验数据表明：鼠肝细胞的ＤＮＡ相对含量分别为精子的ＤＮＡ相对含量的２，４，８倍，符合生物学的规律，说明这套系统的定量结果是可靠的。     

证券投资基金背信行为的法理分析

基金＂老鼠仓＂是指基金投资管理人员违背信托义务进行非法的利益输送,是一种典型的背信行为。对基金背信行为的法理分析主要包含三个方面：首先是基金背信行为的表现及其危害;其次是从行为主体、行为方式、侵害法益等方面对基金背信行为的法律特征的分析;最后是从经济、法律和基金治理结构三个层面揭示基金背信行为的成因。     

Occurrence,distribution and nature of neuropeptide Y in the rat pancreas

Summary Significant quantities of a newly discovered peptide, neuropeptide Y, were found in the rat pancreas, where they were localized to nerves in the exocrine parenchyma and around arterial and ductal structures. Although unaffected by surgical parasympathectomy, the periarterial and periductal nerves were abolished by chemical sympathectomy, suggesting that NPY is partially costored with sympathetic transmitters in nerve fibers.     

Hepatic acid hydrolases of albino rats,Mastomys natalensis and albino mice duringPlasmodium berghei infection

Summary Changes in liver acid hydrolase activities during the infection of albino rats,Mastomys or mice withPlasmodium berghei are described. B-Glucosidase, B-galactosidase and N-acetyl-B-D-glucosaminidase exhibited widely different responses with acid phosphatase and cathepsin-B the least responsive and are likely to be causally related to immunity of animals.     

Effects of polysorbates and Cremophor EL on vascular responses in rat aorta

The effects of Tween 20, Tween 80, and Cremophor EL, surface active agents which are used for dispersion of water-insoluble substances, on vascular responsiveness were investigated using rat aortic rings. In high concentrations all these agents produced persistent contractions in aortic rings independent of the presence of endothelium. These contractions were not influenced by inhibitors of known endogenous contractile mediators. Incubation with these agents caused a concentration-dependent inhibition of the endothelium-dependent relaxant responses to acetylcholine in intact precontracted aortic rings. Endothelium-independent relaxations produced by sodium nitroprusside were not inhibited, but rather potentiated in the presence of Tween 80 (10^–1 ml/l). On the other hand, Tween 80 inhibited the contractile effects of 5-hydroxytryptamine, phenylephrine, and bradykinin significantly. The data suggests that these substances affect both endothelial cells and vascular smooth muscle.     

高原鼠兔（Ochotona curzoniae Hodgson）脊神经的形态解剖

本文在双筒解剖镜下对15只高原鼠兔的脊神经进行了追踪,对每对脊神经的起源、行程及分布作了详细描述,并同家兔和大鼠者进行了比较.发现高原鼠兔的脊神经,除因其椎骨与家兔者不同而引起一些差异外,基本形态与家免几乎一致,有些特征与大鼠也相同.高原鼠兔臂丛和腰荐丛的周围分布,在不同个体间有较大的差异,但有一个基本的分布形态.     

GABA-A-mediated gastrin release induced by baclofen in the isolated vascularly perfused rat stomach

Summary In order to investigate the role of peripheral GABA-B receptors, the effects of the putative GABA-B agonist baclofen on immunoreactive gastrin release from an isolated vascularly perfused rat stomach preparation were examined. The vascular infusion of baclofen at graded concentrations induced a dose-dependent increase in gastrin release; this was unaffected by the GABA-B antagonist delta-aminovaleric acid, but was fully prevented by the selective GABA-A antagonist bicuculline as well as by atropine or tetrodotoxin. These results suggest that the stimulant effects of baclofen are mediated by nervous cholinergic structures, associated with GABA-A receptors, and indicate that this GABA-B agonist must be regarded as a partial agonist of peripheral GABA-A receptors.     

Gene product expression of cyclin D<Subscript>2</Subscript> and p16 during the transition from cardiac myocyte hyperplasia to hypertrophy

The current study was to investigate mRNA expression of cyclin D₂ and p16 during the transition from cardiac myocyte hyperplasia to hypertrophy. Cultured cardiac myocytes (CM) and fibroblasts (FC) obtained from 1-day-old Sparague-Dawley rats were used in this study. We have determined (1) hyperplasia by cell growth curve and fluorescence activated cell sorting (FACS); and (2) ultrastructure by electron microscope observation; and (3) expresions of cyclin D₂ mRNA and p16 mRNA by using in situ hybridization and image analysis. The results were shown (1) Results of cell growth curve and FACS analysis showed CM could proliferate in the first 3 cultured days (4 days in postnatal development). But the ability decreased quickly, concomitant with the differentiation. (2) The ultrastructure of CM showed the large amount of myofilaments and mitochondrion and FC showed moderate amount of rough endoplasmic reticulum. (3) The expression of cyclin D₂ mRNA in 3−, 4−, 5−day CM group was 0.89 times (p<0.05), 0.80 times (p<0.05) and 0.56 times (p<0.01) of that in 1-day group respectively. P16 mRNA in 2−, 3−, 4−, 5−day CM group were 1.63 times (p<0.01), 1.72 times (p<0.01), 1.99 times (p<0.01) and 2.84 times (p<0.01) of that in 1−day group respectively. It can be concluded that cultured neonatal rat cardiac myocytes could proliferate during the first 3 cultured days, but the ability of proliferation decreased, from the fourth day, concomitant with differentiation. Cyclin D₂ and p16 have the key roles during the transition from myocyte hyperplasia to hypertrophy. Biography: Zhang Yu-xia (1974-), female, Master, research direction: cardiovascular pathology.