Immunogenicity of recombinant fowl-pox virus co-expressing structural protein precursor P1-2A and proteinase 3C of FMDV

Two recombinant plasmids, pUTA2P1 and pUTAL3CP1, were constructed by inserting structural protein precursor P1-2A and proteinase 3C of foot-and-mouth disease virus (FMDV) into fowl-pox virus (FPV) recombinant vectors pUTA-2 and pUTA-16-LacZ respectively, and two recombinant FPVs (vUTA2P1 and vUTAL3CP1) screened by the RT-PCR, IFA assay and Western blotting assay were obtained successfully. Mice injected respectively with rFPVs were induced high level specific anti-FMDV antibodies, increasing of T subtypes, and higher cytotoxicities of splenocytes than those of control groups. These results indicated that a new method was used to construct a potential candidate vaccine of FMDV.     

Improved elastase production by Bacillus sp. EL31410—further optimization and kinetics studies of culture medium for batch fermentation

An efficient culture medium producing a bacterial elastase with high yields was developed further following preliminary studies by means of response surface method. Central composite design (CCD) and response surface methodology were applied to optimize the medium constituents. A central composite design was used to explain the combined effect of three medium constituents, viz, glucose, K(2)HPO(4), MgSO(4).7H(2)O. The strain produced more elastase in the completely optimized medium, as compared with the partially optimized medium. The fitted model of the second model, as per RSM, showed that glucose was 7.4 g/100 ml, casein 1.13 g/100 ml, corn steep flour 0.616 g/100 ml, K(2)HPO(4) 0.206 g/100 ml and MgSO(4).7H(2)O 0.034 g/100 ml. The fermentation kinetics of these two culture media in the flask experiments were analyzed. It was found that the highest elastase productivity occurred at 54 hours. Higher glucose concentration had inhibitory effect on elastase production. At the same time, we observed that the glucose consumption rate was slow in the completely optimized medium, which can explain the lag period of the highest elastase production. Some metal ions and surfactant additives also affected elastase production and cell growth.     

谈"天"与中国古人的神学观

"天"是中国人的一个重要哲学及神学概念,在传世典籍里屡见不鲜,但此概念的渊源至少可追溯到甲骨文、金文时代.本文结合甲骨文、金文和传世典籍的相关材料,对我国古人对"天"这一观念的演变作一些初步的梳理,从而探讨一下古人的神学观.     

理想与现实碰撞的火花--《梦游天姥吟留别》赏析

本文赏析了《梦游天姥吟留别》的独特格调。从诗人的怀才不遇的愤懑到瑰丽奇特的“天马行空”式的构思,从诗人丰富想象,大胆夸张的浪漫主义气质到诗人蔑视权贵的傲岸性格,从诗人出类拔萃的材质到娴熟驾驭语言的能力,可以说《梦游天姥吟留别》是诗人理想与现实碰撞的耀眼的火花。     

美国普世主义文化的宗教根源和本土化过程探析

美国普世主义的宗教根源来自基督教的一神信仰和普救论。在普世主义的美国本土化过程中,至关重要的两个理念是“上帝选民论”和“天定命运论”。“上帝选民论”使美国人以上帝选民自居;“天定命运论”给美国的扩张套上神谕的色彩。在可以预见的未来,处于“利他”和“利己”两难境地的美国普世主义价值观,将随其国内外社会政治经济的变化而处于不断调整变化中。     

《三家星经》可以复原成一份完整的星表

敦煌文献P2512号中的《三家星经》缺漏了8座星官,并有一些讹误,依《开元占经》补齐这8座星官后仍缺1星。潘鼐和邓文宽依晋、隋两书《天文志》将其中的天将军列为12星,比《开元占经》多1星,补足了这所缺的1星。受其所缺的1星应在所缺漏的8座星官中的思路的启迪,依《开元占经》中的夹注及按语、日本若杉家本《三家星官簿赞》、朝鲜《天象列次分野之图》等多种文献列文昌为7星,比《开元占经》多1星,仍列天将军为11星而补足了所缺的1星。此外,神宫1星是在《三家星经》之后出现在天文星占典籍中的,应视为中国传统星官的历史嬗变。     

佛教生态思想与中国古代作家的生态审美取向

佛教的生态思想既包含了对解决自然以及人文精神生态问题的可借鉴思想,又为研究古代作家的生态审美取向提供具有哲学认识论意义的思维方式和认知理路。审视中国古代作家从佛教生态思想中所融摄的观念及其所显现的人文精神生态特征,无疑对构建和谐人文精神生态具有现实启示意义。     

L-胱氨酸组装体的STM研究

利用隧道扫描显微镜在室温低真空条件下,在高定向裂解石墨表面对自组装L-胱氨酸及其脱水后状态进行探测.实验表明,吸附在HPOG表面的L-胱氨酸相对完全干燥的组装体表现出更加有序的吸附状态.通过比较含水和脱水样品的单体图形,分析了分子骨架以及内部二硫键的变化.疏水作用、针尖样品的作用以及分子间作用力是诱导样品有序排列的主要原因.高分辨的图形对于吸附在固体表面水溶生物样品的研究,获取分子内部信息很有意义.     

培养条件对康宁木霉产酶的影响

通过对康宁木霉纤维素酶（C1酶和Cx酶）、酸性蛋白酶活力的比较,筛选出了康宁木霉产酶的最适培养条件.康宁木霉在m（麸皮）∶m（秸秆）=2∶8,氮源为w=1%的NH4NO3,含水量为65%,起始pH值为6.5的条件下培养72h,C1酶活力最高,为191.3U/g;在m（麸皮）∶m（秸秆）=6∶4,氮源为w=1%的（NH4）2SO4,含水量为65%,起始pH值为7.5的条件下培养48h,Cx酶活力最高,为2006.5U/g;在m（麸皮）∶m（秸秆）=8∶2,氮源为w=1%的（NH4）2CO3,含水量为65%,起始pH值为4.5的条件下培养96h,酸性蛋白酶活力最高,为1859.6U/g.     

岩心磁组构分析古水流方向的原理与应用

目的针对鄂尔多斯盆地天环拗陷南段延长组开展磁组构古水流研究。方法通过测量岩心样品的磁化率各向异性,分析磁组构特征,结合矿物岩石学特征综合开展岩心古水流方向判识。结果延长组碎屑岩的古水流方向为NE—SW,NW—SE和NS向,分别代表西南、西北和北部3个方向的物质供给。结论磁组构分析古水流方向与轻重矿物组合法判断的物源方向一致,表明应用磁组构分析钻井岩心古水流方向的方法是可行、可靠的,利用磁组构分析方法恢复古水流方向,判识物源具有较好的应用前景。