上海交大等在人类皮肤基因研究领域获得重要成果

近日，中科院上海生命科学研究院和上海交通大学医学院健康科学研究所经过4年多的艰苦努力，在人类皮肤基因研究领域获得重要成果，发现KITLG基因突变引起家族性进行性色素过度沉着症。研究结果已发表在国际学术期刊《美国人类遗传学杂志》（The American Journal of Human Genetics）上。     

“两性人”成因初探

两性人是人类的一种性别畸形,常常给患者带来很多痛苦。本文从人类的性别决定方式和影响性别分化的因素入手,探讨了两性人形成原因的几个方面,并介绍了两性人的检测和治疗情况。     

无脊椎动物进化中的模糊问题思考

无脊椎动物是动物界的低等类型、最庞大类群。本文根据古生物学、现代生物学和生物进化论研究成就，就无脊椎动物起源、三叶虫、头足类、昆虫进化过程中的模糊问题作了逻辑推理思考。     

Effects of truncated mutants of the ε subunit of chloroplast ATP synthase on the fast phase of millisecond delayed light emission of chloroplast and its ATP synthesis ability

The ε subunit of the chloroplast ATP synthase and the truncated ε mutants which lack some amino acid residues from the N-terminus or C-terminus were overexpressed in E. coil When the ε subunit or the truncated ε proteins was added to the spinach chloroplast suspension, both the intensity of the fast phase of millisecond delayed light emission (ms-DLE) and the cyclic and noncyclic photophosphorylation activity of chloroplast were enhanced. With an increase in the number of residues deleted from the N-terminus, the enhancement effect of the N-terminal truncated proteins decreased gradually. For the C-terminal truncated proteins, the enhancement effect increased gradually with an increase in the number of residues deleted from the C-terminus. Besides, the ATP synthesis activity of ε-deficient membrane reconstituted with the ε subunit or the truncated ε proteins was compared. The ATP synthesis activity of reconstituted membrane with the N-terminal truncated proteins decreased gradually as the number of residues deleted from the N-terminus increased. For the C-terminal truncated proteins, the ATP synthesis activity of reconstituted membrane increased gradually with an increase in the number of residues deleted from the C-terminus, but was still lower than that of the wild type ε protein. These results suggested that: (a) the N-terminal domain of the ε subunit of the chloroplast ATP synthase could affect the ATP synthesis activity of ATP synthase by regulating the efficiency of blocking proton leakage of ε subunit; and (b) the C-terminal domain of the ε subunit of the chloroplast ATP synthase had a subtle function in modulating the ATP synthesis ability of ATP synthase.     

Molecular genetic basis for the rhino mouse from Chinese KM subcolony

Both the rhino mouse and hairless mouse resulted from hairless gene mutation, but they show different phenotypes of skin physiology. The rhino mouse has more similar histological characters to human papular alopecia.Therefore rhino mouse is a good experimental animal model for human papular alopecia. This study reports a hairless mouse named rhino KIZ, arose from KM colony in Kunming Institue of Zoology, by systematic studies on morphology,skin histopathology, gene sequence, pedigree and protein domain analysis. The results demonstrate that a C-to-T transition in exon 11 of hr gene (The mutant gene has been applied for a Chinese patent (patent No. 03135280)) results in the rhino KIZ. The rhino KIZ with clear genetic mechanism will be a useful animal model.     

lat基因的失活在提高棒状链霉菌棒酸产量中的应用

利用lat基因突变的重组质粒pKCLHS对紫外诱变的克拉维酸（Clavulanic acid）高产菌株Streptomyces clavuligerusB71—14的胁基因进行了插入失活，对获得的基因突变子进行了PCR验证、菌体生长测定、发酵特征测定，并对发酵液申的克拉维酸进行了初步提取和含量测定．结果表明，突变菌株的lat基因申插入了含有阿泊拉抗性的基因片段，突变菌株的生长速度与原始菌株无明显变化，甜突变菌株的克拉维酸产量最高能达到其原始菌株的1，11～1．29倍，产头霉素C的能力显著降低．     

家族性载脂蛋白B—100缺陷的研究（综述）

家族性载脂蛋白Ｂ－１００缺陷的研究（综述）俞锐敏朱致惠（暨南大学医学院第一附属医院内科，５１０６３０，广州）关键词：载脂蛋白Ｂ－１００；缺陷；基因突变；高胆固醇血症中图分类号：Ｒ５８９－１现已证实低密度脂蛋白胆固醇（ＬＤＬ－Ｃ）在体内的清除通过以下两...     

物种形成理论与现代生物工程

物种形成理论的发展对生物工程进展有直接影响，物种形成理论对生物工程产品的安全性更具有现实指导意义，它将使生物工程向着健康和成熟的机制运转。     

致癌基因知多少?

科学家早已知道，引发癌症的导火索是细胞内基因突变。但大部分致癌突变是通过在显而易见的地方，如对控制细胞分裂的基因的观测而发现的。现在看来，这些观测只是对大幅画卷的瞵间一瞥。     

基因突变检测技术暨第4届变性高效液相色谱技术及其应用国际会议

由兰州大学基础医学院和生命科学院举办的“基因突变检测技术暨第4届变性高效液相色谱（DHPLC）技术及其应用国际学术交流会”于2006年8月19号在兰州开幕。