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961.
P. Auf der Maur 《Cellular and molecular life sciences : CMLS》1972,28(10):839-841
Summary Tightly packed elementary fibrils with a diameter of 20 Å are demonstrated in human metaphase chromosomes in situ.
Für die Mithilfe bei der Präparation danke ich FrauM. Brun del Re; für die Aufnahmen mit dem Philips EM 300 bin ich HerrnJ. di Lullo vom Veternärmedizinischen Institut der Universität Bern zu Dank verpflichtet. 相似文献
Demonstration von Elementarfibrillen in menschlichen Metaphasechromosomen in situ
Für die Mithilfe bei der Präparation danke ich FrauM. Brun del Re; für die Aufnahmen mit dem Philips EM 300 bin ich HerrnJ. di Lullo vom Veternärmedizinischen Institut der Universität Bern zu Dank verpflichtet. 相似文献
962.
G inversion in bacteriophage Mu: a novel way of gene splicing 总被引:17,自引:0,他引:17
963.
Chemotactic and random movement of cord-blood granulocytes 总被引:1,自引:0,他引:1
L Máródi J Jezerniczky S Csorba L Karmazsin P C Leijh R van Furth 《Experientia》1984,40(12):1407-1410
Chemotactic responsiveness and random movement of cord-blood granulocytes were studied with a modified Boyden's method. Cord-blood granulocytes were less active chemotactically than granulocytes from healthy children and adults, whereas the random filter movement of the cells from all three sources was about the same. In cord sera, concentrations of cell directed chemotaxis inhibitors were equal to those in sera from other age groups. Compared with the situation in healthy children and adults, the generation of chemotactic factors in cord-blood sera was impaired. This impairment was not related to an increased activity of chemotactic factor inactivators. Measurement of the cyclic nucleotide levels in granulocytes from cord-blood and from children belonging to various age groups revealed that the cord granulocytes have significantly lower concentrations of cAMP and cGMP, which could have been responsible for the decreased chemotactic responsiveness. 相似文献
964.
Roos Cuperus René Leen Godelieve A. M. Tytgat Huib N. Caron André B. P. van Kuilenburg 《Cellular and molecular life sciences : CMLS》2010,67(5):807-816
Fenretinide induces apoptosis in neuroblastoma by induction of reactive oxygen species (ROS). In this study, we investigated
the role of mitochondria in fenretinide-induced cytotoxicity and ROS production in six neuroblastoma cell lines. ROS induction
by fenretinide was of mitochondrial origin, demonstrated by detection of superoxide with MitoSOX, the scavenging effect of
the mitochondrial antioxidant MitoQ and reduced ROS production in cells without a functional mitochondrial respiratory chain
(Rho zero cells). In digitonin-permeabilized cells, a fenretinide concentration-dependent decrease in ATP synthesis and substrate
oxidation was observed, reflecting inhibition of the mitochondrial respiratory chain. However, inhibition of the mitochondrial
respiratory chain was not required for ROS production. Co-incubation of fenretinide with inhibitors of different complexes
of the respiratory chain suggested that fenretinide-induced ROS production occurred via complex II. The cytotoxicity of fenretinide
was exerted through the generation of mitochondrial ROS and, at higher concentrations, also through inhibition of the mitochondrial
respiratory chain. 相似文献
965.
Paleoproteomic study of the Iceman’s brain tissue 总被引:1,自引:1,他引:0
Frank Maixner Thorsten Overath Dennis Linke Marek Janko Gea Guerriero Bart H. J. van den Berg Bjoern Stade Petra Leidinger Christina Backes Marta Jaremek Benny Kneissl Benjamin Meder Andre Franke Eduard Egarter-Vigl Eckart Meese Andreas Schwarz Andreas Tholey Albert Zink Andreas Keller 《Cellular and molecular life sciences : CMLS》2013,70(19):3709-3722
The Tyrolean Iceman, a Copper-age ice mummy, is one of the best-studied human individuals. While the genome of the Iceman has largely been decoded, tissue-specific proteomes have not yet been investigated. We studied the proteome of two distinct brain samples using gel-based and liquid chromatography–mass spectrometry-based proteomics technologies together with a multiple-databases and -search algorithms-driven data-analysis approach. Thereby, we identified a total of 502 different proteins. Of these, 41 proteins are known to be highly abundant in brain tissue and 9 are even specifically expressed in the brain. Furthermore, we found 10 proteins related to blood and coagulation. An enrichment analysis revealed a significant accumulation of proteins related to stress response and wound healing. Together with atomic force microscope scans, indicating clustered blood cells, our data reopens former discussions about a possible injury of the Iceman’s head near the site where the tissue samples have been extracted. 相似文献
966.
967.
968.
J Borst R J van de Griend J W van Oostveen S L Ang C J Melief J G Seidman R L Bolhuis 《Nature》1987,325(6106):683-688
Cloned blood lymphocytes that do not express the alpha- and beta-chains of the T-cell receptor show MHC-unrestricted cytotoxicity. These cells carry the gamma-protein, disulphide-linked either to another molecule or to itself, and associated with the CD3 complex. These observations may help to solve the mystery posed by the discovery of the gamma gene. 相似文献
969.
E M Sankila R Tolvanen J A van den Hurk F P Cremers A de la Chapelle 《Nature genetics》1992,1(2):109-113
Choroideremia (CHM) is an X-linked progressive degeneration of the choroid and retina. 12% of unrelated male patients carry deletions of the partially cloned CHM gene. In Finland, there are more than 120 living CHM patients belonging to eight apparently unrelated pedigrees. Molecular deletions involving the CHM gene have been detected in three families. We have screened the remaining five families for point mutations. In one large family a single nucleotide (T) insertion into the donor splice site of exon C leads to two aberrantly spliced mRNAs both producing a premature stop codon. The mutation can be assayed easily by amplification and digestion with Msel. Our findings provide additional evidence for the pathogenetic role of CHM mutations and provide a diagnostic tool for one fifth of the world's known CHM patients. 相似文献
970.
G. A. Schuiling N. Pols-Valkhof G. C. J. van der Schaaf-Verdonk F. H. de Jong T. R. Koiter 《Cellular and molecular life sciences : CMLS》1987,43(8):895-898
Summary The mid-pregnancy rescue of corpora lutea can be mimicked in the pseudopregnant rat by induction of decidual tissue in the uterus: in such rats, around day 10, there is neither luteolysis, nor resumption of follicle-development or increase of the pituitary responsiveness to LHRH. The results suggest that the mid-pregnancy rescue of corpora lutea is caused by a maternal factor.12 February 1987 相似文献