Building of hFⅨ transgenic mice by spermatogenic cells

Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor.     

Expression of human clotting factor Ⅸ mediated by recom- binant lentiviral vector in cultured cells and hemophilia B mice

To explore the expression of human clotting factor Ⅸ (hFⅨ) cDNA in vitro and the feasibility of gene therapy for hemophilia B mice mediated by recombinant lentiviral vector, a recombinant hFⅨ lentiviral vector driven by ubiquitin-C promoter, FUXW, and by ABP liver specific promoter, FAXW, was constructed respectively. Recombinant lentivirus was harvested from 293T cells by calcium phosphate-mediated transient cotransfection of three plasmids (transgene vector, CMV腞8.2, VSV-G). hFⅨ expression was detected in supernatant of 293T, BHK and L-02 cells infected with FUXW virus, whereas higher expression of hFⅨ levels (630 ng/106 cells/48 h) was detected only in L-02 cells infected with FAXW virus. Serum hFⅨ antigen was detected in all hemophilia B mice treated with FAXW virus by tail vein injection, an efficiency level of hFⅨ was observed (45 ng/mL, approximately 1% of normal human levels), the expression lasted for more than 60 d. The results indicated that HIV-based lentiviral vectors offer a promising approach to the gene therapy of hemophilia B.     

基于UML的面向对象分析及其应用

统一建模语言 ( Unified Modeling Language,UML)是一种面向对象的建模语言 ,在实际系统建模中被广泛使用 .介绍了基于 UML的面向对象分析方法的基本原理和实现过程 ,并以此分析并设计了一个小型自动售货系统     

微孔草种子化学成分的研究

从微孔草 (MicroulasikkimenisHemsl)种子中首次分离出 8个已知化合物 :β 谷甾醇 (1)、羽扇豆醇 (2 )、白桦脂醇 (3 )、豆甾 4 烯 3 酮 (4)、豆甾 4 烯 3 二酮 (5 )、羟基豆甾 4 烯 3酮 (6)、诺米林 (7)、如忒文 (8) .报道了这8个化合物的分离纯化和结构鉴定     

基于Agent的个性化课件模型

针对目前基于Web的远程教育课件往往无视学习者个性差别的现状，引入Agent技术，利用其智能化的特性构造了一个个性化课件模型，从而合理解决了不同学习者之间在学习上的个性差异，真正作到了因材施教。     

基于有限元仿真结果的知识发现

提出了通过数据挖掘技术实现基于有限元仿真结果的知识发现．分析了数据挖掘的一般步骤，建立了基于有限元仿真结果的知识发现系统，论述了知识发现系统的各个模块及实现该系统的关键技术；对粗糙集和主成分分析法作了简单的介绍，并举实例对粗糙集方法作了说明．通过数据挖掘技术将计算实例提炼出来，作为一种知识源参与到设计优化过程中去，将CAE从设计验证层次提升到设计驱动层次．     

精冲断裂过程的有限元模拟

从材料细观损伤角度对精冲原理进行了研究．开发了基于空穴材料的刚塑性有限元分析系统，该系统可以估算精冲材料的损伤状态，判断材料失效并模拟裂纹的扩展与汇合状况．利用该系统模拟了精冲过程中塑性变形与韧性断裂现象，预测工件断面光亮带高度，研究凸凹模间隙与剪切面质量关系，并与试验数据进行了对比．研究发现，凸凹模间隙与工件剩余厚度之比是影响断面质量的关键因素．     

崇明东滩鸟类自然保护区被子植物区系研究

报道了崇明东滩鸟类自然保护区被子植物名录、植物区系组成成分及特点，并将其与奉贤地区滩涂和九段沙湿地的植物区系进行了比较，分析讨论了东滩植物区系的形成原因及其影响因子。为保护区的生物多样性研究及其保护提供了基础资料。     

带径向函数的粗糙核的Marcinkiewicz积分算子在乘积空间上的有界性

研究了带径向函数的粗糙核的Marcinkiewicz积分算子 μΩ ,b在乘积空间Rn×Rm(n ,m≥ 2 )中的有界性 .在Ω∈L(log+ L) 2 (Sn - 1×Sm - 1) ,b(|x|,|y|)∈l∞(Lq) (R+ ×R+ )条件下 ,证明了 μΩ ,b是Lp(Rn×Rm)有界的 ,这里当 12时 ,q′

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