Genomic instability in Gadd45a-deficient mice.

Gadd45a-null mice generated by gene targeting exhibited several of the phenotypes characteristic of p53-deficient mice, including genomic instability, increased radiation carcinogenesis and a low frequency of exencephaly. Genomic instability was exemplified by aneuploidy, chromosome aberrations, gene amplification and centrosome amplification, and was accompanied by abnormalities in mitosis, cytokinesis and growth control. Unequal segregation of chromosomes due to multiple spindle poles during mitosis occurred in several Gadd45a -/- cell lineages and may contribute to the aneuploidy. Our results indicate that Gadd45a is one component of the p53 pathway that contributes to the maintenance of genomic stability.     

Natural engineering principles of electron tunnelling in biological oxidation-reduction

We have surveyed proteins with known atomic structure whose function involves electron transfer; in these, electrons can travel up to 14 A between redox centres through the protein medium. Transfer over longer distances always involves a chain of cofactors. This redox centre proximity alone is sufficient to allow tunnelling of electrons at rates far faster than the substrate redox reactions it supports. Consequently, there has been no necessity for proteins to evolve optimized routes between redox centres. Instead, simple geometry enables rapid tunnelling to high-energy intermediate states. This greatly simplifies any analysis of redox protein mechanisms and challenges the need to postulate mechanisms of superexchange through redox centres or the maintenance of charge neutrality when investigating electron-transfer reactions. Such tunnelling also allows sequential electron transfer in catalytic sites to surmount radical transition states without involving the movement of hydride ions, as is generally assumed. The 14 A or less spacing of redox centres provides highly robust engineering for electron transfer, and may reflect selection against designs that have proved more vulnerable to mutations during the course of evolution.     

星形胶质细胞低温去分化条件

体外纯化培养的成熟星形胶质细胞经冷冻复苏方法处理后,诱导转化为放射状胶质细胞的类似细胞(RGLCs细胞).星形胶质细胞的诱导转化率与其冷冻过程的温度、时间有关.冷冻保护液中的DMSO对星形胶质细胞的诱导转化过程无关.这些结果显示冷冻复苏方法诱导星形胶质细胞的转化是由物理刺激诱导的.     

硬质合金刀片槽型实物模型参数化设计

提出了刀片槽型参数化设计思想，进而进一步提出了槽型参数化设计的尺寸驱动的参数化设计算法及其在ＡｕｔｏＣＡＤ进行二次开发的实现方法。     

I-内射模及其性质

假设A是交换的Noether环,I是A任意给定的理想,并且满足ht(I)＞0。本文将内射模的概念在一定意义上进行推广,给出I-内射模的概念及其性质,并定义了模的I-内射维数以及环上的整体I-维数。     

专家系统在电力系统中的应用—水电厂全厂运行操作管理实用专家系统

本文介绍一个水电厂全厂运行操作管理实用专家系统。该系统利用近年新兴的人工智能技术，针对水电厂的运行实际需要进行开发，用目前流行的人工智能语言ＴｕｒｂｏＰｒｏｌｏｇ编制而成。该系统已通过鉴定，并投入现场使用，一年多的结果表明：该系统能自动完成水电厂包括电气、油、水、风等系统所有操作票的开票任务，开票迅速，开出的操作票正确实用、清晰美观，对防止误操作，提高全厂运行的安全性起到了良好的作用；同时它为管理人员提供了一种有效的管理手段，有利于防止无票操作，有利于提高工作效率。     

A Cooperative Schema between Web Sever and Search Engine for Improving Freshness of Web Repository

0 IntroductionTIon tceorpneet w ,itthhefr cerqauwelnetr sch oafn sgeeasr cohf tehneg iinnfeo rnemeadti toon uopnd tahteethe web pages visited. Different pages have different changefrequencies[1].Sothe crawlers must firstly esti mate which pa-ges need to b…     

弹性约束非均匀梁振动的传递矩阵法

本文采用有限元素法的基本思想与传递矩阵法相结合，研究了非均匀弹性直梁在端部受有直簧和扭簧约束下的自振问题，给出了适合于计算机计算的最一般格式，由此出发可以导出二十多种不同边界条件下的频率方程，提出了非均匀控制变量参数，使计算工作量大为减少，且误差很小、可靠性高。