Genomic organization of EDSV strain AA-2

The genomic organization of egg drop syndrome virus strain AA-2 isolated in China is reported here. The genome was found to be 32 838 bp in length, approximately 2 kb shorter than those of the human subgenus C adenoviruses. Analysis of open reading frames indicated that the genes for major viral structural proteins (55 K, Penton base, pⅦ, pⅩ, pⅥ, Hexon, 100 K, pⅧ and Fiber), as well as EP, DNA polymerase and ⅣaⅡ are present in the expected locations in the genome. EDSV possesses no identifiable E3 and E4 regions and most proteins encoded by E3 and E4 regions of other adenoviruses genome were not presented in EDSV.     

A continuous 200-ka palaeoclimatic record from stalagmite in Tangshan Cave, Nanjing

An oxygen isotope temperature record from 381 to 166 kaB. P. (²³⁰Th/²³⁴U dating and tuned ages) has been obtained by combining data on the isotopic composition of calcites with that of fluid inclusions trapped in a stalagmite from a limestone cave in which a fossil cranium deposited. Several laminae in microsequence of the stalagmite represent climatic events or shifts. The Nanjing man fossil was older than 381 kaB. P.     

基于双正交小波的快速矢量量化算法

提出了一种用于图象压缩的矢量量化算法．该算法通过构造符合图象小波变换系数特征的跨频带矢量,利用小波系数之间的相关性,提高了图象的编码效率和重构质量．同时,该算法又采用了两种新的矢量量化技术——非线性插补矢量量化（ＮＬＩＶＱ）和渐进构造聚类（ＰＣＣ）,提高了矢量量化的速度和码书质量．实验结果证明,该算法在比特率为０．１７２ｂｐｐ的条件下仍能获得ＰＳＮＲ＞３４ｄＢ的高质量重构图象．研究图象小波变换系数的固有特性是提高矢量量化性能的关键,而提高矢量量化速度是这类算法得以实用的重要前提．     

空化噪声特性实验研究

利用不同类型的轴对称头体和ＮＡＣＡ１６０１２翼型做了一系列空化噪声实验,得到轴对称头体在不同空化数及不同速度下的空化噪声谱及翼型在不同的攻角下的空化噪声谱．这一系列的噪声谱表明,各种轴对称头体的空化噪声总声级随空化数的减小而升高,但噪声谱形具有明显的相似形;翼型在片空化、泡空化和云雾空化状态下所辐射的噪声谱存在明显的差别．噪声总声级的变化较为复杂,由泡空化状态转换到片空化状态,空化数虽然减小,但总声级会突然降低．针对翼型在泡空化状态下所辐射噪声谱的特征,利用单空泡溃灭理论进行了尝试性的解释和说明．     

Cloning and expression of the vp39 gene ofBombyx mori nuclear polyhedrosis virus inE. coli

The nuclear capsid protein gene (vp39) ofBombyx mori nuclear polyhedrosis virus (BmNPV) was amplified successfully by PCR technique and inserted into pGEM 3zf(+). The 5′ and 3′ terminal area of the amplified vp39 gene were sequenced with silver-staining dideoxy method. Bmvp39 gene was sub-cloned into the expression vector pRSET-A, and transformed intoE. coli BL21. This gene was highly expressed by IPTG induction. SDS-PAGE analysis showed that the expressed protein is about 38 kd, and the expressed amount reached maxium in 4 h with IPTG induction. Supported by the National Natural science Foundation of China and the Doctoral Foundation of Edn carto Committee Liu Deli: born in 1954, Doctoral Candidate from Huazhong Normal University To whom correspondence should be addressed: (027-7882712-2938)     

花生油磷酸酯加脂剂的研制及应用

本文报道了以花生油为原料,经酯交换,磷酸化制备新型皮革加脂剂,并研究了其单一及复配后的加脂性能。     

马尔柯夫链状预测的概率计算及拓广

提出了一种马尔柯夫链状预测中的概率计算新方法：状态划分矩阵法;具有模糊状态的马尔柯夫链的概率计算公式,并用实例计算作了说明。     

HCV感染与多种自身抗体高检出率的关系

为探讨丙型肝炎病毒（ＨＣＶ）感染后体内出现的自身抗体的病理意义及其引起的自身免疫的发生机制。方法：对１６１例ＨＣＶ感染者进行了八种自身抗体（抗核抗体、类风湿因子、抗甲状腺球蛋白抗体、抗甲状腺微粒体抗体、抗双链ＤＮＡ抗体、抗ＲＮＰ抗体、抗Ｓｍ抗体、抗精子抗体）的检测。结果：有５２例检出６９项次自身抗体,自身抗体检出率为３２．３％,显著高于健康人对照组（Ｐ＜０．００５）（未计抗精子抗体）。结论：ＨＣＶ感染可能是诱发自身免疫反应的一个重要因素     

腺病毒载体介导胸苷激酶基因/ACV对大鼠脑胶质瘤的离体和活体杀伤作用

建立缺陷型重组腺病毒载体介导的胸苷激酶基因治疗系统（Adtk／ACV）,进行离休和活体治疗大鼠C6脑胶质瘤研究．将质粒pAdtk与pJM17共转染腺病毒包装细胞──293细胞,同源重组后制备纯化的Adtk并以PCR证实;Adtk对离休C6细胞的杀伤作用随着Adtk滴度和ACV剂量增加而增强,并有旁观者效应,而未转染C6细胞和AdLacZ转染C6细胞均未被杀伤;扫描电镜下见Adtk／ACV处理的细胞呈明显病理改变;ACV可有效杀伤Adtk／C6细胞．在大鼠脑立体定向仪引导下于右额叶接种2×106个C6细胞,分别于第3,6,8和10天原位注射Adtk,同时腹腔注射ACV（100mg／（kg·d-1））,3d组、6d组、8d组大鼠成活期在90d以上,组织学检查未发现肿瘤细胞．10d组成活期（28．5±4．6）d,而C6未治疗组和AdLacZ／ACV治疗组成活期分别为（1．8±3．1）d和（14．0±2．2）d．本文建立的Adtk／ACV在离体和活体水平对大鼠C6脑胶质瘤有强烈的杀伤作用,效果确实,应用方便,ACV价格便宜,有潜在临床应用价值．     

基于PKI的RBAC应用

在PKI（PUBLIC Key Infrastructure）技术广泛用于电子商务和电子政务的今天，对网上的资源进行授权服务的需求变得越来越迫切．基于角色的访问控制技术RBAC（Role_based Access Control）有效地解决了权限管理的复杂性，降低了管理开销，还能为管理员提供一个较好的安全政策的环境．本文提出了一种将PKI技术与RBAC技术相结合的方案，实现了基于身份认证的安全访问控制．