Age-dependent response of lactate dehydrogenase of pituitary of rat to testosterone

Summary The effects of time and various doses of testosterone on the responsiveness of lactate dehydrogenase of pituitary of 7-, 38- and 78-week-old rats were studied. The activity of the enzyme increases in 78-week-old rats. Castration decreases the enzyme activity at all ages. Maximum increase in the enzyme activity is seen with 50 and 100 g of testosterone 4 h after administration of hormone to castrated rats. No further time and dose-dependent effect is observed. The magnitude of increase for the enzyme is higher at the age of 38 weeks and decreases in 78-week-old rats.To whom all correspondence should be addressed. The authors are grateful to Professor M.S. Kanungo, Biochemistry Laboratory, Department of Zoology, Banaras Hindu University for the suggestions and encouragement. K.V.G. thanks the PL-480 for a research fellowship.     

Seasonal variations in fat body metabolism of the green frog Rana esculenta (L.).

Rana esculenta (L.), kept under natural conditions, show almost constant body weights in the annual cycle. Fat body weight, however, has a distinct maximum in October decreasing continuously to a May minimum which is also evident in the fat body index. The triglyceride and protein contents show the same course. Suprisingly high amounts of glycogen are found, which reach a maximum in August.     

The effect ofβ-adrenergic stimulation and blockade on the efflux of PGE1-like material from the isolated perfused rabbit heart

Summary Prostaglandin (PG) release was measured from the isolated perfused rabbit heart. The effects of -adrenergic stimulation and blockade suggest that PG synthesis is regulated in part by adrenergic mechanisms.     

Effects of ACTH and diabetes on phospholipid metabolism in adrenal mitochondria.

Incorporation of 32P into adrenal mitochondrial phospholipids (PL) incrased in ACTH-treated rats, but it decreased in diabetics, inspite of the fact that these animals showed adrenal overacity. Since diabetics did not show increased 11 beta-hydroxylation. as opposed to ACTH-treated rats, it is suggested that the stimulation of this enzyme activity by exogenous ACTH is related to an increased turnover of PL at the mitochondrial membrane. The process is impaired in diabetics and prevents the stimulation of 11 beta-hydroxylation.     

A centrally induced vasodepressor response after intravenous administration of whole venom ofNaja mossambica pallida in cats

Summary Naja mossambica pallida venom administered i.v. (300 /kg) produces an initial brief fall in blood pressure, due to a direct myocardial depressant effect, and a sustained fall due to central depressant effect.This study was supported by University of Nairobi research grants (670–376). We also thank Mr E. Njogu for photographic assistance.     

Metabolism of the snailCryptozona ligulata during regeneration of optic tentacles

Summary The biochemical changes in the hemolymph and tissues were followed during regeneration of the optic tentacles of the snailCryptozona ligulata (Pulmonata-Stylommatophora). There is a remarkable increase in total carbohydrates in hemolymph and tissues and glycogen in tissues at the expense of free amino acids and fatty acids. It is clear that ablation of the optic tentacles stimulates carbohydrate synthesis through glyconeogenic routes. The optic tentacles regenerate completely in 18–21 days.     

High-performance reversed-phase liquid chromatography (HPLC) of favism-inducing factors inVicia faba L.

Summary A HPLC assay for L-Dopa, convicine, and vicine in broad-bean extracts has been developed. The distribution of these compounds in the different organs of the plant has been studied, especially in the seeds, where their presence may cause favism if the beans are used as food.Work supported by C.N.R. through Progetto Finalizzato Miglioramento delle produzioni vegetali per fini alimentari ed industriali mediante interventi genetici. Sottogrogetto leguminose da granella. Paper No. 200.     

A prostaglandin-like activity in small intestine and postirradiation gastrointestinal syndrome

Summary A correlation between the postirradiation increase of the small intestine motility and the prostaglandin-like activity in this organ during gastrointestinal syndrome was observed. Indomethacin decreased the elevated motility of intestine and reduced the prostaglandin-like activity in this syndrome.Acknowledgments. The work was supported by the Polish Academy of Sciences.     

The effects of physostigmine on the oxygen uptake in rat brain tissue

Summary Physostigmine in a dose of 0.1 mg/kg i.v. expressly stimulated the oxygen uptake in the rat cerebral cortex. This effect was blocked by propranolol and seems be mediated by catecholamines. Since atropine also antagonized the stimulant effect of physostigmine, it appears that the action of physostigmine is primarily cholinergic and that the adrenergic effect is a secondary phenomenon. The higher dose of physostigmine (0.4 mg/kg i.v.) caused a depression of rat brain oxygen uptake.This work was supported by a grant from Serbian Republic Scientific Fund (ZMNU SR Srbija), Belgrade (Yugoslavia).The authors wish to acknowledge the skilful technical assistance of Lj. Krsti.