高校图书馆知识管理浅谈

知识管理是知识经济时代的一种全新的管理思想。阐述了高校图书馆在对自身实施知识管理时应注意的环节、存在的问题以及所要避免陷入的几个误区，并对未来的知识管理进行了展望。     

城市定位与城镇群建设

城市是一个国家和民族对外开放的窗口、商品国际交换的平台，它决定着整个国家和地区的竞争力。文章对廊坊市城市发展的现状和廊坊市城镇群建设战略进行了论述，希望对我国其他城市的发展有一定的参考作用。     

世界文化和不可知论的政见——一个致力于跨文化融洽交流的计划

就世界文化课程内容的具体设置,及联合大学与传媒以更好地实现课程积极目标等诸问题进行探讨.通过多层次的论证强调指出人们始终持有相异或相冲突的宗教、哲学以及意识形态,尚没有一种普世真理足以使世间所有人心悦诚服.即便是广泛接受和运用中的科学知识,其发展亦受阻于人类本身的局限而不可确定.尽管科学在前沿问题研究上竞争激烈,也仍然无法回答和消除所有的问题和差异.因此,从全球或泛人类立场观之,人类现有的知识与理解能力具有不确定和不完整的特点,而这样的特点浸透在所有的具体文化信仰之中.认识到共同局限性的人们--尤其是政界的领导人--亟需摒弃极端的文化分离思想,以更宽容理解的心态思考和行动,这样才能避免不同民族及不同国家间的不断冲突和互相伤害.     

混凝土工程裂缝原因与预防

混凝土经常出现的裂缝有温度裂缝、干龟裂、干缩裂缝、沉陷裂缝、坍落裂缝。分析了5种裂缝出现的原因，提出了相应的预防措施。     

Growth of Magnetite Nanoparticles under Magnetic Fields

1 Introduction Over the past several years, the preparation and characterization ofnanoscale magnetic materials, especially one-dimensional (1D) nanostructure, have attracted much attention as the nanomaterials would allow investigating the fundamental aspects of magnetic-ordering phenomena in magnetic materials with reduced dimensions and could lead to new potential applications such as data storage technology[1-6].     

New testing methodology for the quantification of rock crushability: Compressive crushing value (CCV)

Crushing is a size reduction process that plays a key role in both mineral processing and crushing-screening plant design. Investigations on rock crushability have become an important issue in mining operations and the manufacture of industrial crusher equipment. The main objective of this research is to quantify the crushability of hard rocks based on their mineralogical and mechanical properties. For this purpose, the mineralogical, physical, and mechanical properties of various hard rocks were determined. A new compressive crushing value (CCV) testing methodology was proposed. The results obtained from CCV tests were compared with those from mineralogical inspections, rock strength as well as mechanical aggregate tests. Strong correlations were found between CCV and several rock and aggregate properties such as uniaxial compressive strength (UCS), the brittleness index (S₂₀), and aggregate impact value (AIV). Furthermore, the relationship between the mineralogical properties of the rocks and their CCVs were established. It is concluded that the proposed testing methodology is simple and highly repeatable and could be utilized as a pre-design tool in the design stage of the crushing process for rock quarries.     

A Proof of the Duality of the DINA Model and the DINO Model

The Deterministic Input Noisy Output “AND” gate (DINA) model and the Deterministic Input Noisy Output “OR” gate (DINO) model are two popular cognitive diagnosis models (CDMs) for educational assessment. They represent different views on how the mastery of cognitive skills and the probability of a correct item response are related. Recently, however, Liu, Xu, and Ying demonstrated that the DINO model and the DINA model share a “dual” relation. This means that one model can be expressed in terms of the other, and which of the two models is fitted to a given data set is essentially irrelevant because the results are identical. In this article, a proof of the duality of the DINA model and the DINO model is presented that is tailored to the form and parameterization of general CDMs that have become the new theoretical standard in cognitively diagnostic modeling.     

Critical role of extracellular vesicles in modulating the cellular effects of cytokines

Under physiological and pathological conditions, extracellular vesicles (EVs) are present in the extracellular compartment simultaneously with soluble mediators. We hypothesized that cytokine effects may be modulated by EVs, the recently recognized conveyors of intercellular messages. In order to test this hypothesis, human monocyte cells were incubated with CCRF acute lymphoblastic leukemia cell line-derived EVs with or without the addition of recombinant human TNF, and global gene expression changes were analyzed. EVs alone regulated the expression of numerous genes related to inflammation and signaling. In combination, the effects of EVs and TNF were additive, antagonistic, or independent. The differential effects of EVs and TNF or their simultaneous presence were also validated by Taqman assays and ELISA, and by testing different populations of purified EVs. In the case of the paramount chemokine IL-8, we were able to demonstrate a synergistic upregulation by purified EVs and TNF. Our data suggest that neglecting the modulating role of EVs on the effects of soluble mediators may skew experimental results. On the other hand, considering the combined effects of cytokines and EVs may prove therapeutically useful by targeting both compartments at the same time.     

Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination

Membrane proteins are key elements in cell physiology and drug targeting, but getting a high-resolution structure by crystallographic means is still enormously challenging. Novel strategies are in big demand to facilitate the structure determination process that will ultimately hasten the day when sequence information alone can provide a three-dimensional model. Cell-free or in vitro expression enables rapid access to large quantities of high-quality membrane proteins suitable for an array of applications. Despite its impressive efficiency, to date only two membrane proteins produced by the in vitro approach have yielded crystal structures. Here, we have analysed synergies of cell-free expression and crystallisation in lipid mesophases for generating an X-ray structure of the integral membrane enzyme diacylglycerol kinase to 2.28-Å resolution. The quality of cellular and cell-free-expressed kinase samples has been evaluated systematically by comparing (1) spectroscopic properties, (2) purity and oligomer formation, (3) lipid content and (4) functionality. DgkA is the first membrane enzyme crystallised based on cell-free expression. The study provides a basic standard for the crystallisation of cell-free-expressed membrane proteins and the methods detailed here should prove generally useful and contribute to accelerating the pace at which membrane protein structures are solved.