城市老旧小区老年人户外活动休息点分布量化研究

为解决城市老旧小区内老年人户外活动休息点建设中易受主观因素影响,且未能真实反映老年人群体需求,无法快速评估和预测休息点的分布问题,通过跟踪记录与问卷访谈的方法,研究了南京市鼓楼区9个老旧小区老年人户外步行活动的特征,提出最优步行距离的概念及其计算方法用于描述特定老年人群体户外活动的水平,对老年人群体的户外活动步行距离进行量化,并以此为基础建立社区老年人户外活动休息点的分布模型,对9个小区休息点的理想分布进行计算,结果表明,该模型有利于客观地评价城市老旧小区中老年人户外活动休息空间的建设状况,有利于快速发现建成小区中休息点分布的不足,有利于直观预测规划设计中的老年人户外休息空间配置水平。     

Preparation of the selenomethionine derivative of tabtoxin resistance protein

In order to obtain phase information for the X-ray diffraction of tabtoxin resistance protein (TTR) crystal using the MAD phasing method, a selenomethionine (Se-Met) derivative of TTR was overexpressed in E. coli strain M15, with pQE-30 plasmid, through IPTG induction in M9 medium containing Se-Met. The product was purified to an estimated homogeneity of greater than 95% according to SDS-PAGE, by a Ni-NTA metal affinity followed by a Mono Q anion exchange column chromatography. The successful substitution of Se-Met for methionine (Met) was confirmed by MALDI-TOF and ESI-Quadrupole Mass Spectrometry analysis. The derivative crystal was obtained using similar conditions as those for the native.     

The new sideway of CNTF signal transduction pathway

The action of ciliary neurotrophic factor (CNTF) on intercellular free Ca²⁺ concentrations [Ca²⁺]_i induced by glutamate (Glu) in primary cultured hippocampal neurons were detected with Fura2/AM, a Ca²⁺-sensitive fluorophore, and the morphological influence of G-protein on it was objected. Glu could induce rapid increase of [Ca²⁺]_i in hippocampal neurons. CNTF had no significant action on [Ca²⁺]_i in resting hippocampal neurons. However, after incubation of CNTF for 5 min, the increase of [Ca²⁺]_i in hippocampal neurons rapidly induced by Glu was inhibited. Pretussis toxin (PTX)-sensitive G protein could block the action. These results indicate that a new non-genomic rapid sideway might exist in the upper stream of CNTF signal transduction pathway, which was related to Ca²⁺ signal transduction. Keywords:     

Geochronology of the Hannan intrusive complex to adjoin the Qinling orogen and its rapid cooling reason

The geochronological works for the Hannan intrusive complex, cosisting of the gabbro-quartz dio-rite-plagiogranite bodies, in the north border of the Yangtze craton block, adjoining the Qinling orogen, are reported, because its age and genesis are of great importance to research of the tectonic evalutional history for the Qinling orogen and of the rifted processes for the Late Proterozoic supercontinent Rodinia. A good isochron of t=(837±26) Ma (2σ), corresponding to INd=0.51165±2(2σ), εNd(t)=+1.9, MSWD1.02, was difined by the Nd isotopic analytical results for the 21 whole-rock samples from the varied lithological complex bodies, but for the Rb-Sr isotopic aralytical data there is no isochron. The 40Ar/39Ar plateau age of the biotite, sorted from plagiogranites, is (796±20) Ma(2σ), and yet for the Rb-Sr isotopic data of the plagiogranite whole-rock samples (WR) and the sorted biotite (Bio), plagioclase (Plag) and apatite (Apt) mineral samples from the plagiogranitewhole-rock samples, an isochron of t=(824.86±3.8) Ma (2σ) with ISr=0.70393±14(2σ), MSWD2.44 is given. The U-Pb isotopic results for the single zircon, sorted from pla-giogranite samples, yielded an upper and a lower intercept ages of -876 Ma and -273 Ma. The Nd isotope data of complex indicate that it is probably the products of crystallization differentiation from the magma with 3333Nd>0 when the rapid uplift in 837-800 Ma took place in the northern border of the Yangtze craton block.     

Determination of interface (surface) dilatation rheological properties

This note establishes a new method that can measure interface dilatation elastic module (e) and dilatation viscosity (d) of all surfactants based on the surface pressure relaxation process. The wide applicability was proved with the stability of foam as an example.     

颗粒增强不锈钢基复合材料冲蚀磨损性能研究

在MMG－200高温氧化-冲刷腐蚀磨损试验机上,考察了Al     

一个用于有机物生物活(毒)性研究的新拓扑指数

提出一个新的拓扑指数Yx,即隐氢图中的所有相邻边的一阶分子连接性指数的乘积之和占分子中有效碳原子的分率,用Yx研究取代苯,甲基苯、卤代苯、卤代烃、氯苯、含氮杂环化合物和直链伯醇的生物活（毒）性,建立相应的相关方程（r=0.9203-0.9914),计算出部分取代苯类化合物对发光菌（Photobacterium phosphoreum)、呆鲦鱼（Piumephales promelass)、大型蚤（Daphnia magua strau)、酵母菌（Saccharomyces cerevisiae)和绿藻Green algae的毒性数据,新的拓扑指数易于计算,有较高的结构区分能力,新方法计算方便,物理意义明确。     

大面积CVD金刚石膜的热铁板抛光

研制成功国内第1台大面积CVD金刚石膜热铁板抛光机,它可以在10^-3pa真空条件下,加热到1100℃;抛光台可以在0－10r/min间实现无级调速,一次完成3片φ110mm的金刚石膜的抛光,金刚石膜在980℃,2h抛光的结果表明该装置有良好的抛光效果。金刚石膜在980℃抛光不同时间的Raman谱表明,金刚石热铁板抛光是金刚石石墨化和C原子不断扩散的过程。     

硼系表面活性剂复配性能的研究

以二乙醇胺与硼酸酯化合成硼酸酯（DEAB）,再与1－溴十二烷烷基化合成了一类新型含硼两性表面活性剂（RDEAB）。用二元表面活性剂溶液的热力学,研究了RDEAB－AES、RDEAB－AEO9 2种水溶液的混合体系胶束中表面性剂分子间相互作用参数β^m和胶束的组成,2种混合体系的β^m值分别为：－6．47、－3．46。     

A defined range of guard cell calcium oscillation parameters encodes stomatal movements.

Oscillations in cytosolic calcium concentration ([Ca2+]cyt) are central regulators of signal transduction cascades, although the roles of individual [Ca2+]cyt oscillation parameters in regulating downstream physiological responses remain largely unknown. In plants, guard cells integrate environmental and endogenous signals to regulate the aperture of stomatal pores and [Ca2+]cyt oscillations are a fundamental component of stomatal closure. Here we systematically vary [Ca2+]cyt oscillation parameters in Arabidopsis guard cells using a 'calcium clamp' and show that [Ca2+]cyt controls stomatal closure by two mechanisms. Short-term 'calcium-reactive' closure occurred rapidly when [Ca2+]cyt was elevated, whereas the degree of long-term steady-state closure was 'calcium programmed' by [Ca2+]cyt oscillations within a defined range of frequency, transient number, duration and amplitude. Furthermore, in guard cells of the gca2 mutant, [Ca2+]cyt oscillations induced by abscisic acid and extracellular calcium had increased frequencies and reduced transient duration, and steady-state stomatal closure was abolished. Experimentally imposing [Ca2+]cyt oscillations with parameters that elicited closure in the wild type restored long-term closure in gca2 stomata. These data show that a defined window of guard cell [Ca2+]cyt oscillation parameters programs changes in steady-state stomatal aperture.