Ethanol influence on insulin secretion from isolated rat islets

Summary This study was done to delineate the role of - and -adrenergic receptors and cyclic AMP in the mechanism of ethanol effects on insulin release from isolated islets. Rats were given an -adrenergic blocker, phentolamine, or a -adrenergic blocker, propranolol. In addition, ethanol 1 g/kg was given intragastrically 1 h prior to sacrifice. Glucose mediated insulin release from isolated islets was enhanced by phentolamine and decreased by propranolol. Ethanol treatment inhibited glucose-induced insulin release from isolated islets of control rats as well as those given phentolamine and/or propranolol. Insulin release from isolated islets in response to dibutyryl-cyclic AMP was attenuated by ethanol. Theophylline enhanced glucose mediated insulin release from control islets but ethanol treatment produced a significant inhibition of insulin response. The data suggest that the site of action of the deleterious effects of ethanol on insulin release from isolated islets in rat does not involve adrenergic system and cyclic AMP.Supported by the U.S. Veterans Administration     

‘Lord only of the ruffians and fiends’? William Whewell and the plurality of worlds debate

By the middle of the nineteenth century, the opinion of science, as well as of philosophy and even religion, was, at least in Britain, firmly in the camp of the plurality of worlds, the view that intelligent life exists on other celestial bodies. William Whewell, considered an expert on science, philosophy and religion (among other areas), would have been expected to support this position. Yet he surprised everyone in 1853 by publishing a work arguing strongly against the plurality view. This was even stranger given that he had endorsed pluralism twenty years earlier in his contribution to the Bridgewater Treatises. In this paper I show that the shift in Whewell’s view was motivated by three factors: the influence of Richard Owen’s theory of archetypes on Whewell’s view of the argument from design, and Whewell’s perception of the need to strengthen such arguments in light of evolutionary accounts of human origins; important developments in his view of philosophy and his role as a scientific expert; and new findings in astronomy. An examination of the development of Whewell’s position provides a lens through which we can view the interplay of theology, philosophy and science in the plurality of worlds debate.     

Analysis of yeast protein kinases using protein chips

We have developed a novel protein chip technology that allows the high-throughput analysis of biochemical activities, and used this approach to analyse nearly all of the protein kinases from Saccharomyces cerevisiae. Protein chips are disposable arrays of microwells in silicone elastomer sheets placed on top of microscope slides. The high density and small size of the wells allows for high-throughput batch processing and simultaneous analysis of many individual samples. Only small amounts of protein are required. Of 122 known and predicted yeast protein kinases, 119 were overexpressed and analysed using 17 different substrates and protein chips. We found many novel activities and that a large number of protein kinases are capable of phosphorylating tyrosine. The tyrosine phosphorylating enzymes often share common amino acid residues that lie near the catalytic region. Thus, our study identified a number of novel features of protein kinases and demonstrates that protein chip technology is useful for high-throughput screening of protein biochemical activity.