Hepatocyte growth factor-mediated attraction of mesenchymal stem cells for apoptotic neuronal and cardiomyocytic cells

Human bone marrow-derived mesenchymal stem cells (MSC) home to injured tissues and have regenerative capacity. In this study, we have investigated in vitro the influence of apoptotic and necrotic cell death, thus distinct types of tissue damage, on MSC migration. Concordant with an increased overall motility, MSC migrated towards apoptotic, but not vital or necrotic neuronal and cardiac cells. Hepatocyte growth factor (HGF) was expressed by the apoptotic cells only. MSC, in contrast, revealed expression of the HGF-receptor, c-Met. Blocking HGF bioactivity resulted in significant reduction of MSC migration. Moreover, recombinant HGF attracted MSC in a dose-dependent manner. Thus, apoptosis initiates chemoattraction of MSC via the HGF/c-Met axis, thereby linking tissue damage to the recruitment of cells with regenerative potential.     

Regulation of the cardiac sodium pump

In cardiac muscle, the sarcolemmal sodium/potassium ATPase is the principal quantitative means of active transport at the myocyte cell surface, and its activity is essential for maintaining the trans-sarcolemmal sodium gradient that drives ion exchange and transport processes that are critical for cardiac function. The 72-residue phosphoprotein phospholemman regulates the sodium pump in the heart: unphosphorylated phospholemman inhibits the pump, and phospholemman phosphorylation increases pump activity. Phospholemman is subject to a remarkable plethora of post-translational modifications for such a small protein: the combination of three phosphorylation sites, two palmitoylation sites, and one glutathionylation site means that phospholemman integrates multiple signaling events to control the cardiac sodium pump. Since misregulation of cytosolic sodium contributes to contractile and metabolic dysfunction during cardiac failure, a complete understanding of the mechanisms that control the cardiac sodium pump is vital. This review explores our current understanding of these mechanisms.     

Distributional information on birds from egg sets collected by Henry Rogers Durkee in 1870 in southwestern Wyoming

Henry Rogers Durkee collected 74 egg sets of 27 avian species in 1870 at Gilmer, Uinta County, in southwestern Wyoming. Despite the paucity of documented breeding evidence from this region during the late 19th and early 20th centuries, his material at the Smithsonian Institution was generally overlooked and has never been critically examined. Durkee's egg sets included 5 species (Sandhill Crane, Grus candensis ; Northern Rough-winged Swallow, Stelgidoteryx serripennis ; Grasshopper Sparrow, Ammodramus savannarum ; Fox Sparrow, Passerella iliaca ; Cassin's Finch, Carpodacus cassinii ) whose breeding distribution was then poorly known, 25 to more than 70 years before nests and eggs were otherwise documented in Wyoming. Durkee also collected complete egg sets of Lark Bunting ( Calamospiza melanocorys ) over 70 years before breeding was confirmed at other peripheral locations in Wyoming. In addition, Durkee's incomplete egg sets of Grasshopper Sparrow were the 1st western subspecies ( A. s. perpallidus ); his complete egg set and nest of the Fox Sparrow collected at Gilmer constituted the 2nd locality for the species or species group ( P. i. schistacea ). Although the number of egg sets Durkee collected is modest, he made a meaningful contribution to the early history of avifaunal exploration in Wyoming.