审视秘书活动主体行为美的内涵

秘书人员的行为美表现为工作主体的朴实性、规范性、和谐性、创造性 ,其中创造性是秘书行为美的最高层次 ,是秘书工作本质力量的体现     

微生物实验教学中学生能力培养和科学素质教育

阐述微生物实验教学加强大学生的能力培养和科学素质教育及提高实验队伍的总体素质     

A calcium sensor in the sodium channel modulates cardiac excitability.

Sodium channels are principal molecular determinants responsible for myocardial conduction and maintenance of the cardiac rhythm. Calcium ions (Ca2+) have a fundamental role in the coupling of cardiac myocyte excitation and contraction, yet mechanisms whereby intracellular Ca2+ may directly modulate Na channel function have yet to be identified. Here we show that calmodulin (CaM), a ubiquitous Ca2+-sensing protein, binds to the carboxy-terminal 'IQ' domain of the human cardiac Na channel (hH1) in a Ca2+-dependent manner. This binding interaction significantly enhances slow inactivation-a channel-gating process linked to life-threatening idiopathic ventricular arrhythmias. Mutations targeted to the IQ domain disrupted CaM binding and eliminated Ca2+/CaM-dependent slow inactivation, whereas the gating effects of Ca2+/CaM were restored by intracellular application of a peptide modelled after the IQ domain. A naturally occurring mutation (A1924T) in the IQ domain altered hH1 function in a manner characteristic of the Brugada arrhythmia syndrome, but at the same time inhibited slow inactivation induced by Ca2+/CaM, yielding a clinically benign (arrhythmia free) phenotype.     

统计等效多项式非线性系统识别

在平稳激振下的一个非线振动系统，若其响应也是平衡随机过程，该系统可统计等效为一多项多非线性系统，本文介绍统计等效三次多项式的参数估计，系统的多维频率响应应函数以及多项式非线性系统响应的谱分析及谱分析。     

新型连续装载机工作机构的图解设计法

本文介绍了新型连续装载机工作机构图解设计的一般规律,对某些技术参数的确定作了初步探讨。     

Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis

To develop a gene therapy strategy for treating bovine mastitis, a new mammary-specific vector containing human lysozyme (hLYZ) cDNA and kanamycin resistance gene was constructed for intramammary expression and clinical studies. After one time acupuncture or intracisternal infusion of healthy cows with 400 μg of the p215C3LYZ vector, over 2.0 μg/ml of rhLYZ could be detected by enzymatic assay for about 3 weeks in the milk samples. Western blotting showed that rhLYZ secreted into milk samples from the vector-injected cows had molecular weight similar to that of the natural hLYZ in human colostrums. Twenty days after the primary injection, the quarters were re-injected with the same vector by quarter acupuncture and even higher concentrations of rhLYZ could be detected. Indirect competitive ELISA of milk samples showed that the vector injection did not induce detectable humoral immune response against hLYZ. Clinical studies showed that twice acupuncture of quarters with the p215C3LYZ vector had overt therapeutic effect on clinical and subclinical mastitis previously treated with antibiotics, including disappearance of clinical symptoms and relatively high microbiological cure rates. These data provide a solid rationale for using the vector to develop gene therapy for treating bovine mastitis.     

填埋场淋滤液水位对边坡防渗膜拉应力的影响

上、下界面的不平衡摩擦力将导致填埋场边坡上的土工防渗膜承受拉应力．垃圾填埋体内淋滤液水位的高低将对防渗膜上、下界面摩擦力的大小产生影响，进而影响防渗膜承受的拉应力，文中利用作者推导的一种确定防渗膜拉应力大小的新方法对其进行了计算．对某填埋场实例的计算结果表明，淋滤液水位的存在将改善防渗膜的工作性态．     

乙酸乙酯分离竹子中木质素制浆造纸研究

用乙酸乙酯浸提竹子制取纸浆，并用造纸行业相关国家标准对纸浆进行了分析。探讨了乙酸乙酯浓度、反应温度、反应时间、原料与溶剂的固液比对纸浆质量的影响。实验结果表明：当乙酸乙酯浓度为60％、反应温度为180℃、反应时间2h、原料-溶剂比为1：6时，制得的纸浆中残余木素含量相对较低、纸浆得率较高。