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排序方式: 共有266条查询结果,搜索用时 234 毫秒
261.
The genome of the mesopolyploid crop species Brassica rapa 总被引:21,自引:0,他引:21
Wang X Wang H Wang J Sun R Wu J Liu S Bai Y Mun JH Bancroft I Cheng F Huang S Li X Hua W Wang J Wang X Freeling M Pires JC Paterson AH Chalhoub B Wang B Hayward A Sharpe AG Park BS Weisshaar B Liu B Li B Liu B Tong C Song C Duran C Peng C Geng C Koh C Lin C Edwards D Mu D Shen D Soumpourou E Li F Fraser F Conant G Lassalle G King GJ Bonnema G Tang H Wang H Belcram H Zhou H Hirakawa H Abe H Guo H Wang H Jin H Parkin IA Batley J Kim JS Just J Li J Xu J Deng J Kim JA Li J Yu J Meng J Wang J Min J 《Nature genetics》2011,43(10):1035-1039
We report the annotation and analysis of the draft genome sequence of Brassica rapa accession Chiifu-401-42, a Chinese cabbage. We modeled 41,174 protein coding genes in the B. rapa genome, which has undergone genome triplication. We used Arabidopsis thaliana as an outgroup for investigating the consequences of genome triplication, such as structural and functional evolution. The extent of gene loss (fractionation) among triplicated genome segments varies, with one of the three copies consistently retaining a disproportionately large fraction of the genes expected to have been present in its ancestor. Variation in the number of members of gene families present in the genome may contribute to the remarkable morphological plasticity of Brassica species. The B. rapa genome sequence provides an important resource for studying the evolution of polyploid genomes and underpins the genetic improvement of Brassica oil and vegetable crops. 相似文献
262.
Khor CC Davila S Breunis WB Lee YC Shimizu C Wright VJ Yeung RS Tan DE Sim KS Wang JJ Wong TY Pang J Mitchell P Cimaz R Dahdah N Cheung YF Huang GY Yang W Park IS Lee JK Wu JY Levin M Burns JC Burgner D Kuijpers TW Hibberd ML;Hong Kong–Shanghai Kawasaki Disease Genetics Consortium;Korean Kawasaki Disease Genetics Consortium;Taiwan Kawasaki Disease Genetics Consortium;International Kawasaki Disease Genetics Consortium;US Kawasaki Disease Genetics Consortium;Blue Mountains Eye Study 《Nature genetics》2011,43(12):1241-1246
Kawasaki disease is a systemic vasculitis of unknown etiology, with clinical observations suggesting a substantial genetic contribution to disease susceptibility. We conducted a genome-wide association study and replication analysis in 2,173 individuals with Kawasaki disease and 9,383 controls from five independent sample collections. Two loci exceeded the formal threshold for genome-wide significance. The first locus is a functional polymorphism in the IgG receptor gene FCGR2A (encoding an H131R substitution) (rs1801274; P = 7.35 × 10(-11), odds ratio (OR) = 1.32), with the A allele (coding for histadine) conferring elevated disease risk. The second locus is at 19q13, (P = 2.51 × 10(-9), OR = 1.42 for the rs2233152 SNP near MIA and RAB4B; P = 1.68 × 10(-12), OR = 1.52 for rs28493229 in ITPKC), which confirms previous findings(1). The involvement of the FCGR2A locus may have implications for understanding immune activation in Kawasaki disease pathogenesis and the mechanism of response to intravenous immunoglobulin, the only proven therapy for this disease. 相似文献
263.
264.
Jeon J Park SY Chi MH Choi J Park J Rho HS Kim S Goh J Yoo S Choi J Park JY Yi M Yang S Kwon MJ Han SS Kim BR Khang CH Park B Lim SE Jung K Kong S Karunakaran M Oh HS Kim H Kim S Park J Kang S Choi WB Kang S Lee YH 《Nature genetics》2007,39(4):561-565
Rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. Despite the explosion in the number of genome sequences available, such a platform does not exist for filamentous fungi. Here we present the development and application of a functional genomics and informatics platform for a model plant pathogenic fungus, Magnaporthe oryzae. In total, we produced 21,070 mutants through large-scale insertional mutagenesis using Agrobacterium tumefaciens-mediated transformation. We used a high-throughput phenotype screening pipeline to detect disruption of seven phenotypes encompassing the fungal life cycle and identified the mutated gene and the nature of mutation for each mutant. Comparative analysis of phenotypes and genotypes of the mutants uncovered 202 new pathogenicity loci. Our findings demonstrate the effectiveness of our platform and provide new insights on the molecular basis of fungal pathogenesis. Our approach promises comprehensive functional genomics in filamentous fungi and beyond. 相似文献
265.
Kim SH Juhnn YS Kang S Park SW Sung MW Bang YJ Song YS 《Cellular and molecular life sciences : CMLS》2006,63(7-8):930-938
The E5 oncoprotein of human papillomavirus (HPV) 16 plays an important role in early cervical carcinogenesis. Vascular endothelial
growth factor (VEGF) plays a central role in switching on the angiogenic phenotype during early cervical carcinogenesis. However,
the relationship between E5 and VEGF has not previously been examined. To clarify the regulatory role of E5 in VEGF expression,
we transferred the E5 gene into various cell types. E5 increased VEGF expression. The addition of epidermal growth factor
receptor (EGFR) inhibitor significantly suppressed VEGF expression, demonstrating that E5 stimulates VEGF expression through
the activation of EGFR. E5-mediated EGFR activation was accompanied by phosphorylation of Akt and ERK1/2, which are also involved
in VEGF expression. Furthermore, the mRNA stability of VEGF was not affected by E5, but VEGF promoter activity could be modulated
by inhibitors of the EGFR, MEK-ERK1/2 and PI3K/Akt pathways in E5-expressing cells. Collectively, these novel results suggest
that HPV 16 E5 increases VEGF expression by activating EGFR, MEK/ERK1/2 and PI3K/Akt.
Received 23 November 2005; received after revision 10 January 2006; accepted 9 February 2006 相似文献
266.
The vertebrate planar cell polarity (PCP) pathway has previously been found to control polarized cell behaviors rather than cell fate. We report here that disruption of Xenopus laevis orthologs of the Drosophila melanogaster PCP effectors inturned (in) or fuzzy (fy) affected not only PCP-dependent convergent extension but also elicited embryonic phenotypes consistent with defective Hedgehog signaling. These defects in Hedgehog signaling resulted from a broad requirement for Inturned and Fuzzy in ciliogenesis. We show that these proteins govern apical actin assembly and thus control the orientation, but not assembly, of ciliary microtubules. Finally, accumulation of Dishevelled and Inturned near the basal apparatus of cilia suggests that these proteins function in a common pathway with core PCP components to regulate ciliogenesis. Together, these data highlight the interrelationships between cell polarity, cellular morphogenesis, signal transduction and cell fate specification. 相似文献