Interaction of reelin signaling and Lis1 in brain development

Loss-of-function mutations in RELN (encoding reelin) or PAFAH1B1 (encoding LIS1) cause lissencephaly, a human neuronal migration disorder. In the mouse, homozygous mutations in Reln result in the reeler phenotype, characterized by ataxia and disrupted cortical layers. Pafah1b1(+/-) mice have hippocampal layering defects, whereas homozygous mutants are embryonic lethal. Reln encodes an extracellular protein that regulates layer formation by interacting with VLDLR and ApoER2 (Lrp8) receptors, thereby phosphorylating the Dab1 signaling molecule. Lis1 associates with microtubules and modulates neuronal migration. We investigated interactions between the reelin signaling pathway and Lis1 in brain development. Compound mutant mice with disruptions in the Reln pathway and heterozygous Pafah1b1 mutations had a higher incidence of hydrocephalus and enhanced cortical and hippocampal layering defects. Dab1 and Lis1 bound in a reelin-induced phosphorylation-dependent manner. These data indicate genetic and biochemical interaction between the reelin signaling pathway and Lis1.     

Expression of recombinant human lysozyme in the milk of transgenic mice

Human lysozyme is a 130-aa (amino acid) alkaline polypeptide, and has both anti-bacterial and anti-viral properties which make it an important component of human natural immunity system. As a first step toward the ultimate goal of improving the anti-bacterial properties of bovine and ovine milk, a transgenic mouse that contains the genomic DNA sequence of the human lysozme gene has been generated for the first time. From 83 mice generated by microinjection, a total of 6 positive transgenic mice were identified by PCR and Southern blot. F1 mice positive for transgene in lines were also detected by PCR. This shows that transgene could be transmitted from founder transgenic mice to their offspring. Recombinant human lysozyme (rHlys) was found in the whey of 3 female positive transgenic mice by Western blot. The highest concentration of rHlys for transgenic mice was 0.2mg/mL. The antibacterial activity of the whey for transgenic mice was highly enhanced up to 0.4 times as much as that of human, while that of non-transgenic mouse was very low. Although the lysozyme activity of transgenic mice is still lower than that of human, the rHlys exhibits the same specific activity as that of human lysozyme. It provides a strong basis for further studies into the possible application of rHlys express in mammary gland.     

物流系统的合理化研究

指出物流系统合理性提出的原因,并论述了物流系统合理性的体现、作用及途径。     

浅析工程项目中的风险识别方法

文章在分析了项目工程风险的基础上，提出了工程项目风险辨识的一般方法，并对几种常用的方法进行了分析。     

浅谈公路工程质量管理

通过对影响工程质量的因素分析,到如何控制施工质量,并对原材料的检验、配合比设计、施工工艺、机械设备、效益分析等方面,及建设单位、施工单位、监理单位对整体工程的关注与实施进行了分析、论述。     

影响磁头超薄气膜飞行稳态特性因素的数值分析

利用有限差分原理对修正后的气体雷诺方程进行离散,并以剪切流为迭代主项求解获得了超薄气膜润滑轴承的压力分布.在此基础上,通过改变磁头初始工作条件,如气膜特征高度、初始俯仰角、磁盘旋转速度,分别计算得到了气膜轴承在稳态下的飞行特性参数,包括气浮力、气浮力转矩、最大气浮力、最小气浮力等.文中还详细分析了这些初始工作条件对稳态下磁头飞行特性参数的影响.计算结果表明:随着气膜特征高度的降低和初始俯仰角的减小,气体轴承刚度增大;气膜特征高度不变时转速变化对特性参数的影响与俯仰角不变时转速对特性参数的影响具有相同的变化趋势.     

城市固体垃圾热解设备与特性研究

针对无筛分城市垃圾热解的实用技术进行了研究,开发出内热源和外热源联合使用的垃圾热解设备.同时对设备的温度变化、垃圾热解特性、产气规律以及影响热解气热值的主要因素等进行了详细的分析.结果表明:实用热解设备的产气率高,理想热解温度在700℃以下.垃圾热解气中H2的体积分数达15.2%,C2H4,C2H6,C3H6和CH4的体积分数总和是10%,热解气的平均热值为7546.4 kJ/m3.设备运行稳定且温度特性满足设计要求.     

煤矿复杂多源数据的混合相似性度量方法

随着煤矿产业自动化、电子化和信息化的发展,目前已有的数据描述方式不能有效刻画煤矿中多源、多维、动态的海量数据信息.针对该问题,首先将源于哲学范畴中的本体引入煤矿生产领域,以矿区监测位置本体作为语义模型与数值描述相结合,形成树状层次结构来描述煤矿复杂多源数据;然后,通过基于语义和数值的混合相似性度量方法计算煤矿数据的相似度;最后通过实验验证了本文所述方法的可行性和有效性.     

一类高维Laplace方程的拟周期解

本文利用 KAM 理论,证明了一类带有常位势 m(m>2) 以及 Fourier 乘子Mζ的高维Laplace 方程△u (m Mζ)u u3=0,在周期边值条件下拟周期解的存在性.     

关于n-余挠模

设 R 为环,M 为右 R 模,n是一个给定的非负整数.若对任意平坦右R模 N 都有Ext n 1 R (N, M) = 0则称M 为 n-余挠模.若对任意n-余挠右R-模 N都有 Ext1R(M, N) = 0则称M为n-平坦模.本文给出了n-余挠模与n-平坦模的一些性质.