Functional characterization of cadmium-responsive garlic gene AsMT2b： A new member of metallothionein family

METALLOTHIONEINS(MT)ARE LOW-MOLECULAR-WEIGHT AND CYSTEINE-RICH PROTEINS.THE FIRST MT PROTEIN WAS ISOLATED FROM EQUINE RENAL CORTEX IN1957[1].GENES EN-CODING MTS OCCUR IN DIVERSE ORGANISMS SUCH AS ANIMALS,HIGHER PLANTS,EUKARYOTIC MICROORGANISMS AND IN SOME…     

PLC在永磁直线同步电机提升系统中的应用

针对永磁直线同步电动机(PMLSM)垂直提升系统的特点,介绍了PLC控制系统在PMLSM系统中的应用,提出通过PLC控制整个工作流程,利用组态软件构建上位监控系统的设计方案,并对系统的主要功能和组成,软件及硬件的实现进行论述.     

长白山北坡森林生态系统的生物生产量及化学能研究

通过对长白山北坡森林生态系统的生物生产量及化学能的测量，揭示长白山北坡森林生态系统能量的分配规律。研究结果表明，长白山北坡森林生态系统中以针阔混交林生物量最高，人工落叶松林的生产量最高，并得出红松（Pinus koraiensis）和水曲柳（Fraxinus mandshurica）的化学能模型，从而为合理开发利用森林资源提供科学依据。     

变结构与变参数电力系统短路故障的快速计算

本文提出了变结构与变参数电力系统短路故障口的各序等值电路参数和口电流的一种新的数字仿真计算模型。同时只需详细计算单相接地短路电流的电气量值,利用同序口电流比例系数,便可快速算出同一位置其它各型短路电气量值。本方法完全避免了各序网节点导纳或阻抗矩阵的修正计算与各型故障计算中的重复计算,具有通用、精确和快速计算的特点。     

金雕肾脏及肝细胞的超微结构观察

本文对金雕肾脏和肝细胞的超微结构作了初步观察,结果表明:金雕肾脏具有极周细胞,其内含丰富的膜包颗粒.肾小体之滤过屏障结构与家鸡相似.肝细胞具发达的粗面内质网.     

小特征比等温湍流矩形射流的试验研究

本文通过实验对小特征比(2,4,8)的矩形管道喷口射流作了研究;给出了热线风速仪的测量结果,即近场区的时均特性和湍流特性,包括轴心线上的时均速度衰减和湍流量的变化、射流横截面上的时均速度和湍流量的分布、射流半宽度特性及主副轴的转换特性.文中将所得结果与前人完成的大特征比矩形射流的实验结果做了对比,并进行了有益的讨论.     

Intersections of two offset parametric surfaces based on topology analysis

Conventional methods for solving intersections between two offset parametric surfaces often include iteratively using computationally expensive SSI (surface/surface intersections) algorithm. In addition, these methods ignore the relations between the intersection curves of parametric surfaces with different offset distances. The algorithm presented in this paper, makes full use of the topological relations between different intersection loops and calculates intersection loops with the help of previously calculated intersection loops. It first pre-processes two parametric surfaces to obtain the characteristic points, called topology transition points (TTPs), which can help in the subsequent finding of the topologies of the intersection curves. Then these points are categorized into several distinct groups, and we can determine the calculation strategy for searching initial points by analyzing the properties of these TTPs on the surfaces. Hence, all intersection curves can be marched from initial points by the tracing algorithm. The proposed algorithm could calculate intersection curves robustly and effectively, and has been tested to be capable of overcoming the degenerate conditions such as loop and singularities leaking that occur frequently in conventional algorithms.     

黄河沉积物中重金属离子的形态转化及释放研究

以黄河包头段上游清洁河段的沉积物为吸附剂,以Pb^2 、Cu^2 、Zn^2 、Cd^2 等多离子溶液为吸附质,开展了重金属离子被黄河沉积物吸附后的再释放,以及吸附作用对重金属形态转化的影响等实验研究．结果表明,重金属离子被黄河沉积物吸附后,各元素均不转入残渣态,Cu^2 和Zn^2 主要向碳酸盐结合态及铁锰氧化物结合态转化,Pb^2 主要向碳酸盐结合态和可交换态转化;Cd^2 主要转入可交换态和碳酸盐结合态．吸附后的赋存形态决定了Cd^2 的释放量及释放能力远远大于其它3种重金属离子,由Cd^2 排放引起的污染不易消除且影响长久．     

Regulatory defects in liver and intestine implicate abnormal hepcidin and Cybrd1 expression in mouse hemochromatosis

Individuals with hereditary hemochromatosis suffer from systemic iron overload due to duodenal hyperabsorption. Most cases arise from a founder mutation in HFE (845G-->A; ref. 2) that results in the amino-acid substitution C282Y and prevents the association of HFE with beta2-microglobulin. Mice homozygous with respect to a null allele of Hfe (Hfe-/-) or homozygous with respect to the orthologous 882G-->A mutation (Hfe(845A/845A)) develop iron overload that recapitulates hereditary hemochromatosis in humans, confirming that hereditary hemochromatosis arises from loss of HFE function. Much work has focused on an exclusive role for the intestine in hereditary hemochromatosis. HFE deficiency in intestinal crypt cells is thought to cause intestinal iron deficiency and greater expression of iron transporters such as SLC11A2 (also called DMT1, DCT1 and NRAMP2) and SLC11A3 (also called IREG1, ferroportin and MTP1; ref. 3). Published data on the expression of these transporters in the duodenum of HFE-deficient mice and humans are contradictory. In this report, we used a custom microarray to assay changes in duodenal and hepatic gene expression in Hfe-deficient mice. We found unexpected alterations in the expression of Slc39a1 (mouse ortholog of SLC11A3) and Cybrd1, which encode key iron transport proteins, and Hamp (hepcidin antimicrobial peptide), a hepatic regulator of iron transport. We propose that inappropriate regulatory cues from the liver underlie greater duodenal iron absorption, possibly involving the ferric reductase Cybrd1.     

Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR

Nanoparticle PCR is a novel method to optimize DNA amplification. It performs well in improving specificity, enhancing sensitivity and speed. Several mechanisms were proposed in previous studies: one was based on the interaction between gold nanoparticles (AuNPs) and DNA while the other was attributed to the heat transfer property of AuNPs. In this paper, we propose that the interaction between AuNPs and DNA polymerase can significantly influence PCR. First, the addition of DNA polymerase can eliminate the inhibitory effects of excess AuNPs. Second, the addition of AuNPs will increase yield of the desired PCR product and make the optimum concentration of DNA polymerase move to higher value. Third, while excess polymerase might inhibit amplification efficiency, AuNPs can reverse this process and the yield of PCR amplification. Based on these results we propose a possible mechanism that AuNPs might modulate the activity of polymerase and improve PCR amplification.