Metabolic studies of Hg-203 on Chlamydomonas reinhardi

Sterile cultures of Chlamydomonas reinhardi, WT+, were treated with Hg-203 at 25 degrees C to identify probably formed volatile mercury compounds. Experiments were performed with living and dead cells under aerobic or anaerobic conditions, respectively, and the mercury concentration was measured in the system algae/nutrient medium. We found a timerelated decrease of mercury concentration in the cell suspension and the cell-free nutrient medium due to a reduction of Hg++ to Hg0, probably caused by extracellular enzymes; monomethyl or dimethyl mercury could not be detected.     

Coordination of branch orientation and photosynthetic physiology in the Joshua tree ( Yucca brevifolia )

Despite the profusion of light in deserts, morphological adaptations to increase light interception are common among desert plants. We studied branch orientation and related physiological parameters in the Mojave Desert Joshua tree, Yucca brevifolia (Agavaceae). Azimuth and inclination were measured on all leaf rosettes of 44 Y. brevifolia trees. Interception of solar radiation was modeled for leaves in hypothetical rosettes facing due south and due north in December, March, and June. Carbon isotope discrimination, nitrogen content, and conductance of water vapor were measured in leaves from north- and south-facing rosettes. Rosette azimuths were nonrandom; rosettes predominantly faced southeast. North-facing rosettes were more steeply inclined than those facing south. The preponderance of south-facing rosettes reduces self-shading and increases interception of solar radiation during the winter-spring growth period. Stomatal conductance was higher for leaves in south-facing than in north-facing rosettes. Nevertheless, discrimination against 13 C was less in leaves of south-facing rosettes, indicating that average intercellular CO 2 concentration was also lower. South-facing whorls had higher leaf nitrogen content. Greater allocation of nitrogen to leaves in south-facing whorls probably results in those leaves having a greater photosynthetic capacity than their north-facing counterparts. Orientation of rosettes to increase interception of sunlight during the period most favorable for photosynthesis, coupled with allocation of nutrients to maintain a higher photosynthetic capacity in those rosettes, should significantly increase whole-plan carbon gain in Y. brevifolia .     

Size, structure, and habitat characteristics of populations of Braya humilis var. humilis (Brassicaceae): an alpine disjunct from Colorado

Size, structure, and habitat characteristics were studied in three populations of Braya humilis var. humilis (C. A. Meyer) Robins, in Gray & Wats. (Brassicaceae), a small, herbaceous perennial of the alpine tundra in central Colorado. There was a significant association between numbers of reproductive, juvenile, and seedling individuals and population location. Plant size within reproductive, juvenile, and seedling size classes varied significantly among three populations. Plots containing Braya had significantly lower total plant cover, a different set of dominant plant species, more rock, bare ground, and less litter than plots without Braya . Braya appears to be restricted to calcareous substrates that experience a moderate level of disturbance, such as solifluction lobes and abandoned roads. Populations are small despite the existence of much potential habitat. Population studies are necessary for active conservation management of Braya .     

Conservation, status, and life history of the endangered White River spinedace, Lepidomeda albivallis (Cyprinidae)

Lepidomeda albivallis (White River spinedace), a fish species endemic to the White River, Nevada, appeared headed toward extinction. In 1991 only 1 population remained, and it comprised fewer than 50 individuals in a 70-m stream reach. We monitored population recruitment and distribution and studied life history and habitat use from 1993 through 1998. We determined that L. albivallis was not reproducing and was continuing to decline, and as an emergency measure we relocated the population (14 in spring 1995 and 6 in spring 1996) downstream 200 m to a secure habitat that we judged more favorable for reproduction. The relocated population reproduced, and by September 1998 it had increased to 396 individuals that inhabited more than 1 km of stream including both pond and stream habitats. In streams they oriented near the bottom but frequently moved up in the water column to strike at drift items. Gut analysis of museum specimens indicated L. albivallis is omnivorous but feeds primarily upon aquatic invertebrates. Conservation of L. albivallis will require reestablishing additional populations within its former range.     

Systematic documentation and analysis of human genetic variation in hemoglobinopathies using the microattribution approach

We developed a series of interrelated locus-specific databases to store all published and unpublished genetic variation related to hemoglobinopathies and thalassemia and implemented microattribution to encourage submission of unpublished observations of genetic variation to these public repositories. A total of 1,941 unique genetic variants in 37 genes, encoding globins and other erythroid proteins, are currently documented in these databases, with reciprocal attribution of microcitations to data contributors. Our project provides the first example of implementing microattribution to incentivise submission of all known genetic variation in a defined system. It has demonstrably increased the reporting of human variants, leading to a comprehensive online resource for systematically describing human genetic variation in the globin genes and other genes contributing to hemoglobinopathies and thalassemias. The principles established here will serve as a model for other systems and for the analysis of other common and/or complex human genetic diseases.     

Disruption of mouse Slx4, a regulator of structure-specific nucleases, phenocopies Fanconi anemia

The evolutionarily conserved SLX4 protein, a key regulator of nucleases, is critical for DNA damage response. SLX4 nuclease complexes mediate repair during replication and can also resolve Holliday junctions formed during homologous recombination. Here we describe the phenotype of the Btbd12 knockout mouse, the mouse ortholog of SLX4, which recapitulates many key features of the human genetic illness Fanconi anemia. Btbd12-deficient animals are born at sub-Mendelian ratios, have greatly reduced fertility, are developmentally compromised and are prone to blood cytopenias. Btbd12(-/-) cells prematurely senesce, spontaneously accumulate damaged chromosomes and are particularly sensitive to DNA crosslinking agents. Genetic complementation reveals a crucial requirement for Btbd12 (also known as Slx4) to interact with the structure-specific endonuclease Xpf-Ercc1 to promote crosslink repair. The Btbd12 knockout mouse therefore establishes a disease model for Fanconi anemia and genetically links a regulator of nuclease incision complexes to the Fanconi anemia DNA crosslink repair pathway.     

Natural variation at Strubbelig Receptor Kinase 3 drives immune-triggered incompatibilities between Arabidopsis thaliana accessions

Accumulation of genetic incompatibilities within species can lead to reproductive isolation and, potentially, speciation. In this study, we show that allelic variation at SRF3 (Strubbelig Receptor Family 3), encoding a receptor-like kinase, conditions the occurrence of incompatibility between Arabidopsis thaliana accessions. The geographical distribution of SRF3 alleles reveals that allelic forms causing epistatic incompatibility with a Landsberg erecta allele at the RPP1 resistance locus are present in A. thaliana accessions in central Asia. Incompatible SRF3 alleles condition for an enhanced early immune response to pathogens as compared to the resistance-dampening effect of compatible SRF3 forms in isogenic backgrounds. Variation in disease susceptibility suggests a basis for the molecular patterns of a recent selective sweep detected at the SRF3 locus in central Asian populations.     

Book Reviews: Behavior Change in the Human Services: An Introduction to Principles and Applications by Martin Sundel and Sandra S. Sundel. Exploring Personality: An Intellectual Odyssey by Russell L. Ackoff. The Ecologist: The Cosmic Covenant.

Systemic Practice and Action Research -