GCC box in<Emphasis Type="Italic">Arabidopsis PDF1.2</Emphasis> promoter is an essential and sufficient cis-acting element in response to MeJA treatment

The expression of Arabidopsis PDF1.2 gene isregulated by jasmonic acid (JA) and ethylene (ET). It also has been well documented that GCC box is an element responsive to ET, however, the responsive mechanism of JA in such plant defense gene expression is unclear. In this paper, the authors define the essential cis-acting element in PDF1.2 promoter responsive to methyl jasmonate (MeJA) through fragment deletions and site-directed mutageneses combiningAgrobacterium-mediated transient reporter gene expression in tobacco leaves. Firstly, the MeJA inducible expression o fPDF1.2 was confirmed by using the upstream -1.86 kb fragment of PDFI.2 gene. Secondly, the upstream -300— -243 bp fragment of the promoter was evidenced to respond to MeJA. To further characterize this promoter region, three point mutations were introduced into the -300— -243 bp fragment of the promoter. This result showed that the mutation of GCC box abolished MeJA induction, whereas the mutations of the G box-like and the imperfect palindrome sequence did not significantly decrease MeJA inducible effect, indicating that GCC box in PDFI.2 is essential for MeJA induction. The sufficient responsiveness to MeJA of this GCC box was further investigated by 4xGCC fused upstream to the CaMV 35S minimal promoter. This result suggested that the fused promoter was able to activate reporter gene expression in response to MeJA. Thus these results indicate that the GCC box in PDFI.2 is an essential and sufficient element to confer MeJA induction.     

Self-assembled structure of alkyloxy substituted benzoic acid methyl ester on HOPG：An STM study

Self-assembled structures of 3,4,5-tris-dodecy-loxy benzoic acid methyl ester (E12), 3,4,5-tris-tetradecy-loxy-benzoic acid methyl ester (E14) and their mixture (E12/E14) have been studied on HOPG by scanning tunneling microscopy (STM). Dimer-like patterns induced by dipole-dipole interaction are observed in E12 and E14 monolayers. The molecules form close-packed rows and interdigitate with the alkyl chains in adjacent molecules. The structural differences are proposed to be from the different length of substituted alkyl chains. Owing to similar adsorption energy, phase separation is observed in the E12 and E14 mixed adlayer with different domains.     

An improved spatially aware routing algorithm for mobile ad hoc network in inter-vehicle communication

A new algorithm called spatially aware routing algorithm with enhanced learning (SAREL) is proposed to guarantee the rationality of route selecting in inter-vehicle communication scenario. Firstly, the traffic model is discussed and set up by using Poisson distribution. Then we analyze the process of traffic evaluation with enhanced learning, and exploit movement estimation to assist state memorization. The improvement of algorithm is provided at last compared with our previous work. Simulation results show that SAREL algorithm could achieve better performance in packet delivery ratio, especially when network connection ratio is average.     

Query through heterogeneous ontologies using association matrix

This paper introduces the definition and calculation of the association matrix between ontologies, h uses the association matrix to describe the relations between concepls ill different ontologics and uses concept vectors to represent queries; then computes the vectors with the association matrix in order to rewrite queries, This paper proposes a simple method of querying through heterogeneous Ontology using association matrix. This method is based on the correctness of approximate information filtering theory; and it is simple to be implemented and expected to run quite fast.     

Early association of electrocardiogram alteration with infarct size and cardiac function after myocardial infarction

OBJECTIVE: Myocardial infarction (MI) is the main cause of heart failure, but the relationship between the extent of MI and cardiac function has not been clearly determined. The present study was undertaken to investigate early changes in the electrocardiogram associated with infarct size and cardiac function after MI. METHODS: MI was induced by ligating the left anterior descending coronary artery in rats. Electrocardiograms, echocardiographs and hemodynamic parameters were assessed and myocardial infarct size was measured from mid-transverse sections stained with Masson's trichrome. RESULTS: The sum of pathological Q wave amplitudes was strongly correlated with myocardial infarct size (r = 0.920, P < 0.0001), left ventricular ejection fraction (r = -0.868, P < 0.0001) and left ventricular end diastolic pressure (r = 0.835, P < 0.0004). Furthermore, there was close relationship between MI size and cardiac function as assessed by left ventricular ejection fraction (r = -0.913, P < 0.0001) and left ventricular end diastolic pressure (r = 0.893, P < 0.0001). CONCLUSION: The sum of pathological Q wave amplitudes after MI can be used to estimate the extent of MI as well as cardiac function.     

Mathematical modeling of salt-gradient ion-exchange simulated moving bed chromatography for protein separations

The salt-gradient operation mode used in ion-exchange simulated moving bed chromatography (SMBC) can improve the efficiency of protein separations. A detailed model that takes into account any kind of adsorption/ion-exchange equilibrium, salt gradient, size exclusion, mass transfer resistance, and port periodic switching mechanism, was developed to simulate the complex dynamics. The model predictions were verified by the experimental data on upward and downward gradients for protein separations reported in the literature. All design and operating parameters (number, configuration, length and diameter of columns, particle size, switching period, flow rates of feed, raffinate, desorbent and extract, protein concentrations in feed, different salt concentrations in desorbent and feed) can be chosen correctly by numerical simulation. This model can facilitate the design, operation, optimization, control and scale-up of salt-gradient ion-exchange SMBC for protein separations.     

网络文化对高校德育教育的影响及对策

通过对皖西学院计算机系专业学生上网情况的深入调查,以班会的形式来教育学生,以“网络给我们带来了什么”这个主题来展开讨论,让学生在讨论中提高认识。同时我们系教师主动出击,挺进网络阵地,开拓了德育教育的新天地。在看到网络文化给我们计算机系学生德育工作带来挑战与冲击的同时,也看到了它作为一个新的育人环境,新的教育模式,新的工作手段和新的工作栽体的巨大潜力,充分发挥网络的优势,用丰富的德育内容,健康的班级文化,独具一格的班级网页,直接快捷的共同聊天室和班主任电子信箱,将网络转化为培养学生健康成长的前沿阵地,成为熏陶学生思想健康发展的崭新领域,正是我们高等学校学生德育工作者与时俱进、开拓进取,探索德育最佳途径的新目标和努力方向。     

THE TCF BLEACHING OF NS-AQ ASPEN HIGH YIELD CHEMICAL PULP

INTRODUCTIONNowadays, under the pressure of energy crisis, Methods押The pulp was put in the high-density polyethylene plastic bag in the of thermostatic water bath where the pulp was treated with chelated environment pollution and market competition, the pulp industry has being developed in the direction of "Three Highness" and " Three Lowness". "Three Highness" means high yield, high strength and high brightness; on the other hand, " Three Lowness" means low energy consumption, low wa…