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991.
Liou YC Sun A Ryo A Zhou XZ Yu ZX Huang HK Uchida T Bronson R Bing G Li X Hunter T Lu KP 《Nature》2003,424(6948):556-561
The neuropathological hallmarks of Alzheimer's disease and other tauopathies include senile plaques and/or neurofibrillary tangles. Although mouse models have been created by overexpressing specific proteins including beta-amyloid precursor protein, presenilin and tau, no model has been generated by gene knockout. Phosphorylation of tau and other proteins on serine or threonine residues preceding proline seems to precede tangle formation and neurodegeneration in Alzheimer's disease. Notably, these phospho(Ser/Thr)-Pro motifs exist in two distinct conformations, whose conversion in some proteins is catalysed by the Pin1 prolyl isomerase. Pin1 activity can directly restore the conformation and function of phosphorylated tau or it can do so indirectly by promoting its dephosphorylation, which suggests that Pin1 is involved in neurodegeneration; however, genetic evidence is lacking. Here we show that Pin1 expression is inversely correlated with predicted neuronal vulnerability and actual neurofibrillary degeneration in Alzheimer's disease. Pin1 knockout in mice causes progressive age-dependent neuropathy characterized by motor and behavioural deficits, tau hyperphosphorylation, tau filament formation and neuronal degeneration. Thus, Pin1 is pivotal in protecting against age-dependent neurodegeneration, providing insight into the pathogenesis and treatment of Alzheimer's disease and other tauopathies. 相似文献
992.
Lou Qin |jian Sarem Mu dar Li Rui |xuan Xiao Wei |jun Lu Zheng |ding Chen Chuan |bo School of Computer Science Technology Huazhong University of Science Technology Wuhan Hubei China 《武汉大学学报:自然科学英文版》2003,8(1):21-27
0 IntroductionTheneedforintegrationofdifferentdatabasemanagementsystems (DBMSs) ,makingdifferencesbetweenthesesystemsinvisibleandprovidinguserswithauniformaccesstoallthedatabaseshasgrownrapidlyduringthelastdecade .However,databaseautonomyandheterogeneity… 相似文献
993.
Lu?PingEmail author Liu?Bin Zhang?Zhe Wu?Feng Deng?Nan-shengEmail author 《武汉大学学报:自然科学英文版》2003,8(4):1147-1152
Inclusion complex of Orange II with β-Cyclodextrin (β-CD) and the anti-photolysis effect under UV-light were investigated.
The molar ratio of inclusion complex of β-Cyclodextrin and Orange II is 1∶1. The formation constantK = 1.236×103 L/mol was determined by the UV and Fluorescence spectra respectively, which was quite in accordance with the calculation
with a modified Benesi-Hildbrand equation. The inclusion complex was characterized by the IR spectra and the molar ratio of
inclusion complex is 1∶1 too. The formation constantK = 1.266×103 L/mol was determined by1H NMR analysis and was nearly the same by UV and fluorescence spectra. The photocatalytic decolorization rate of Orange II
solutions containing β-CD and TiO2 was smaller by 51.9% than that of the Orange II solutions only containing TiO2, while in the case of direct photolysis of Orange II solutions, β-CD can lower the photolysis rate by 48.1% under UV-light.
This result indicates β-CD can inhibit the photolysis and photocatalytic decolorization of Orange II under UV-light. The β-CD
inclusion complex was found to be persistent to UV-light photolysis.
Foundation item: Supported by the National Natural Science Foundation of China (20177017)
Biography: Lu Ping (1958), male. Associate professor, research direction: environmental photochemistry. 相似文献
994.
Li Jin |chai Guo Huai |xi Lu Xian |feng Zhang Zhi |hong Ye Ming |sheng School of Physics Technology Wuhan University Wuhan Hubei China 《武汉大学学报:自然科学英文版》2003,8(3):821-824
0 IntroductionArtificialarthrosiseshavebeenwidelyusedintheoperatorsclinicalsurgeriesforthepatientswhosearthrosiseswerese riouslyseverelydamaged.Butmostofartificialarthrosisescanon lybeusedforabouttenyearslongduetotheseriousabrasion .Forthisreason ,theimprovementofthelongevityofartificialarthrosisbecomesanimportantsubjectofstudy .TheartificialarthrosisesbeingmadeupofTialloyTC4(Ti 6Al 4V)andthepolythenewithultrahighmolecularweighthavegreatpotentialitiesonim provinglongevitybecauseTC4canreac… 相似文献
995.
Polycystins 1 and 2 mediate mechanosensation in the primary cilium of kidney cells 总被引:27,自引:0,他引:27
Nauli SM Alenghat FJ Luo Y Williams E Vassilev P Li X Elia AE Lu W Brown EM Quinn SJ Ingber DE Zhou J 《Nature genetics》2003,33(2):129-137
Several proteins implicated in the pathogenesis of polycystic kidney disease (PKD) localize to cilia. Furthermore, cilia are malformed in mice with PKD with mutations in TgN737Rpw (encoding polaris). It is not known, however, whether ciliary dysfunction occurs or is relevant to cyst formation in PKD. Here, we show that polycystin-1 (PC1) and polycystin-2 (PC2), proteins respectively encoded by Pkd1 and Pkd2, mouse orthologs of genes mutated in human autosomal dominant PKD, co-distribute in the primary cilia of kidney epithelium. Cells isolated from transgenic mice that lack functional PC1 formed cilia but did not increase Ca(2+) influx in response to physiological fluid flow. Blocking antibodies directed against PC2 similarly abolished the flow response in wild-type cells as did inhibitors of the ryanodine receptor, whereas inhibitors of G-proteins, phospholipase C and InsP(3) receptors had no effect. These data suggest that PC1 and PC2 contribute to fluid-flow sensation by the primary cilium in renal epithelium and that they both function in the same mechanotransduction pathway. Loss or dysfunction of PC1 or PC2 may therefore lead to PKD owing to the inability of cells to sense mechanical cues that normally regulate tissue morphogenesis. 相似文献
996.
In this paper, the Life Cycle of Urban Development was firstly analyzed, and the phases of Life Cycle Assessment applied to Urban Development (ULCA) were described. As a case study, ULCA was applied in the environmental impact assessment of the land readjustment project of Hyogo District of Saga, Japan. In addition, mitigation proposals for reducing CO2 were also presented and the relevant environmental ef-fects were simulated. 相似文献
997.
提出一种新的竖向弹簧—钢球隔震系统隔离水平地震作用的隔震方法,用势能不变值原理与“对号入座”法则建立该隔震系统的振动方程,分析各控制参数对该隔震系统地震反应的影响.研究结果表明,该隔震系统结构简单,隔离水平地震作用效果显著. 相似文献
998.
Real-time detection of electron tunnelling in a quantum dot 总被引:3,自引:0,他引:3
Nanostructures in which strong (Coulomb) interactions exist between electrons are predicted to exhibit temporal electronic correlations. Although there is ample experimental evidence that such correlations exist, electron dynamics in engineered nanostructures have been observed directly only on long timescales. The faster dynamics associated with electrical currents or charge fluctuations are usually inferred from direct (or quasi-direct) current measurements. Recently, interest in electron dynamics has risen, in part owing to the realization that additional information about electronic interactions can be found in the shot noise or higher statistical moments of a direct current. Furthermore, interest in quantum computation has stimulated investigation of quantum bit (qubit) readout techniques, which for many condensed-matter systems ultimately reduces to single-shot measurements of individual electronic charges. Here we report real-time observation of individual electron tunnelling events in a quantum dot using an integrated radio-frequency single-electron transistor. We use electron counting to measure directly the quantum dot's tunnelling rate and the occupational probabilities of its charge state. Our results provide evidence in favour of long (10 micros or more) inelastic scattering times in nearly isolated dots. 相似文献
999.
虚拟企业协作博弈中的双优策略 总被引:13,自引:0,他引:13
讨论了目前供应链中的虚拟企业的基本概念及出现的背景 ,介绍了动态联盟为基础的敏捷制造模式 ,从对偶线性规划和双层规划的思想出发 ,在虚拟企业中共赢策略的基础上提出双优的实施策略 ,论述了达到双优所需要的条件及可行性 ,最后讨论了供应链的敏捷性 . 相似文献
1000.
Fan H Lu Y Stump A Reed ST Baer T Schunk R Perez-Luna V Lopez GP Brinker CJ 《Nature》2000,405(6782):56-60
Living systems exhibit form and function on multiple length scales and at multiple locations. In order to mimic such natural structures, it is necessary to develop efficient strategies for assembling hierarchical materials. Conventional photolithography, although ubiquitous in the fabrication of microelectronics and microelectromechanical systems, is impractical for defining feature sizes below 0.1 micrometres and poorly suited to pattern chemical functionality. Recently, so-called 'soft' lithographic approaches have been combined with surfactant and particulate templating procedures to create materials with multiple levels of structural order. But the materials thus formed have been limited primarily to oxides with no specific functionality, and the associated processing times have ranged from hours to days. Here, using a self-assembling 'ink' we combine silica-surfactant self-assembly with three rapid printing procedures--pen lithography, ink-jet printing, and dip-coating of patterned self-assembled monolayers--to form functional, hierarchically organized structures in seconds. The rapid-prototyping procedures we describe are simple, employ readily available equipment, and provide a link between computer-aided design and self-assembled nanostructures. We expect that the ability to form arbitrary functional designs on arbitrary surfaces will be of practical importance for directly writing sensor arrays and fluidic or photonic systems. 相似文献