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91.
Gout AM;ADPKD Gene Variant Consortium Ravine D Harris PC Rossetti S Peters D Breuning M Henske EP Koizumi A Inoue S Shimizu Y Thongnoppakhun W Yenchitsomanus PT Deltas C Sandford R Torra R Turco AE Jeffery S Fontes M Somlo S Furu LM Smulders YM Mercier B Ferec C Burtey S Pei Y Kalaydjieva L Bogdanova N McCluskey M Geon LJ Wouters CH Reiterova J Stekrová J San Millan JL Aguiari G Del Senno L 《Nature genetics》2007,39(4):427-428
92.
1 Results The photosynthetic bacterial reaction center (RC) is a membrane protein complex.The RC is composed of three protein subunits and redox components such as bacteriochlorophylls, bacteriopheophytins,and quinones.The RC performs the photochemical electron transfer from the bacteriochlorophyll dimer through a series of electron donor and acceptor molecules to a secondary quinone,QB.QB accepts electrons from a primary quinone,QA,in two sequential electron transfer reactions.The second electron trans... 相似文献
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源语言调试器是程序员不可缺少的软件开发工具。本文从介绍UNIX系统程序调试的基本思想入手,较详细地分析了UNIX系统源语言调试器的实现,阐述了源语言调试器实现中的几个主要问题。 相似文献
96.
Transgenesis in fish 总被引:1,自引:0,他引:1
Gene transfer into fish embryo is being performed in several species (trout, salmon, carps, tilapia, medaka, goldfish, zebrafish, loach, catfish, etc.). In most cases, pronuclei are not visible and microinjection must be done into the cytoplasm of early embryos. Several million copies of the gene are generally injected. In medaka, transgenesis was attempted by injection of the foreign gene into the nucleus of oocyte. Several reports indicate that the injected DNA was rapidly replicated in the early phase of embryo development, regardless of the origin and the sequence of the foreign DNA. The survival of the injected embryos was reasonably good and a large number reached maturity. The proportion of transgenic animals ranged from 1 to 50% or more, according to species and to experimentators. The reasons for this discrepancy have not been elucidated. In all species, the transgenic animals were mosaic. The copy number of the foreign DNA was different in the various tissues of an animal and a proportion lower than 50% of F1 offsprings received the gene from their parents. This suggests that the foreign DNA was integrated into the fish genome at the two cells stage or later. An examination of the integrated DNA in different cell types of an animal revealed that integration occurred mainly during early development. The transgene was found essentially unrearranged in the fish genome of the founders and offsprings. The transgenes were therefore stably transmitted to progeny in a Mendelian fashion. Southern blot analysis revealed the presence of possible junction fragments and also of minor bands which may result from a rearrangement of the injected DNA. In all species, the integrated DNA appeared mainly as random end-to-end concatemers. In adult trout blood cells, a small proportion of the foreign DNA was maintained in the form of non-integrated concatemers, as judged by the existence of end fragments. The transgenes were generally only poorly expressed. The majority of the injected gene constructs contained essentially mammalian or higher vertebrates sequences. The comparison of the expression efficiency of these constructs in transfected fish and mammalian cells indicates that some of the mammalian DNA sequences are most efficiently understood by the fish cell machinery. Chloramphenicol acetyl transferase gene under the control of promoters from Rous sarcoma virus, and human cytomegalovirus, was expressed in several tissues of transgenic fish. Chicken delta-crystallin gene was expressed in several tissues of transgenic fish.(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献
97.
信托制度最根本的特点是“所有权和利益相分离”。信托财产的独立性使商事信托成为其他法律制度如代理或合同所不能替代的法律制度。从效率的角度出发,证明信托制度的科学性和实用性及其法理价值,并对构建独持养老金制度和集合投资信托制度的商事设计进行探讨。 相似文献
98.
城市规划辅助审批系统中的相似度研究 总被引:2,自引:1,他引:1
城市规划的审批是一件难于量化的工作,它受政治经济等多方面因素影响,是城市规划领域关注的一大焦点.本文尝试将CBR技术用于城市规划辅助审批,让政府的审批依据达到历史与现实的统一.实现CBR的一个关键是相似度的设计,本文结合城市规划辅助审批领域的特点,设计了可行的方案,并应用于实际的办公系统中. 相似文献
99.
激发数学学习兴趣的策略 总被引:1,自引:0,他引:1
针对多数学生对学习数学有枯燥、乏味的感觉,阐述了必须在教学中采用生动的富有感染力的教学方法,以激发学生对数学学习的浓厚兴趣,使学生在提出问题和解决问题中养成善于思考,勇于探索的良好品质。 相似文献
100.
生物液晶对CaCO3结构影响的超声效应 总被引:1,自引:0,他引:1
应用D/max-ⅢC型全自动X射线衍射仪对常温下几种化学合成的CaCO3结构进行了研究.结果表明,一般化学法合成的CaCO3以方解石结构存在;在液晶相中合成的CaCO3以霰石结构存在;而在用超声处理过的液晶相中合成的CaCO3既有方解石结构存在,又有霰石结构存在 相似文献