Archaeal acylamino acid releasing enzyme/lipase:Crystallization and preliminary crystallographic analysis in a new crystal form

A primitive orthorhombic crystal form of acylamino acid releasing enzyme‘lipase(APE1547)from hyperthermophilic archaeon Aeropyrum pernix strain K1 has been obtained at 291K.The diffraction pattern of the crystal extends to 0.27nm resolution at 100K using Cu Kαradiation.The crystal belongs to the space group P212121 with unit cell dimensions of b=6.399,b=10.439and c=16.953nm.The presence of two molecules per asymmetric unit gives a crystal volume per protein mass(Vm)of 0.0022nm^3 Da^-1 and a solvent content of 43% by volume.A full set of X-ray diffraction data were collected to 0.3nm from the native crystal.     

Cryptomelane（KxMn8-xO16）： Natural active octahedral molecular sieve（OMS-2）

The Xiangtan manganese deposit (XTM) used to be considered a supergene oxide manganese ore in South China. We reported a new identification of the naturally outcropping cryptomelane by examining the physical, chemical and structural features of the XTM supergene oxide manganese ore. The MnO2 content was over 90%, K2O more than 3%, and water from 2.2%-3.1% which is similar to one in zeolite. The cell parameters of the cryptomelane were given as a0 = 0.9974 nm, b0 = 0.2863 nm, c0 = 0.9693 nm and β= 91.47°. There was a larger pseduotetragonal tunnel in the natural cryptomelane that was formed by [MnO6] octahedral double chains with aperture of 0.462×0.466 nm2, filled with K cations resulting in some Mn3+ substituting for Mn4+ to balance the negative charges of structure. The finding is important not only for prospecting manganese resources in South China, but also in application of octahedral molecular sieve of natural cryptomelane as that developed in the tetragonal molecular sieve of natural zeolite o     

Combustion energies and standard molar enthalpies of formation for the complexes of the first-row transitional metal chlorides withL-α-histidine

Seven novel solid complexes of the first-row transitional metal with L-α-histidine were synthesized, and their compositions were determined. The constant-volume combustion energies of the complexes were measured by a precision rotation bomb calorimeter. The standard molar enthalpies of combustion and the standard molar enthalpies of formation were calculated. The results indicated that the plots of the standard enthalpies of formation against the atomic number of the metal show a regularity of zigzag.     

Stress corrosion cracking and its anisotropy of a PZT ferroelectric ceramics

It is known that some types of ceramics, like Al2O3 and ZrO2, undergo stress corrosion cracking (SCC) or static fatigue fracture under static loading, ever in a rela-tively mild environment, such as moist atmosphere or water at room temperature, which leads to a time-dependent fracture[1—4]. For a transmission electron microscopy specimen of YBa2Cu3O7-x ceramics superconductor, SCC induced by residual stress can occur in an atmosphere with low moisture content[5]. For a lead zirconate ti…     

磷钼杂多酸催化合成乳酸正丁酯

本探讨了用磷钼酸作催化剂，以乳酸和正丁醇为原料合成乳酸正丁醋，通过对各种酸醇比，催化剂用量，不同反应时间的正交试验，确定了最佳反应条件。     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

运用遗传算法的深亚微米输出缓冲器优化设计方法

分析了小尺寸效应对深亚微米器件性能的影响，结合输入输出耦合电容和漏极扩散层寄生电容对CMOS反相器延迟影响很大的特点，建立了小尺寸器件延时估算模型．采用变尺寸率反相器级连方法，建立了深亚微米输出缓冲器优化设计模型，并运用遗传算法建立了新的优化方法．该方法利用罚函数将小尺寸输出缓冲器优化问题转化为无约束问题，通过适应度函数设计和染色体编码，得到遗传优化结果，克服了传统方法处理非线性多变量问题时存在的效率降低等缺陷．SPICE仿真表明，应用新的深亚微米缓冲器设计模型及方法的优化结果与传统设计比较，延时减少了1个数量级以上，尺寸减小了30%以上，验证了新的深亚微米缓冲器设计模型及设计方法的可靠性．     

缸内直喷汽油机高压涡旋喷油器的油束模型

根据燃油薄膜模型和表面波破碎理论，在KIVA3程序的基础上建立了适合于计算缸内直喷汽油机高压涡旋喷油器油束特性的模型。应用此模型计算出了油束特性的主要参数，包括贯穿度、油束整体和轴向横截面上的索特平均直径等，并研究了高压涡旋喷油器在不同喷射压力和不同背压下的油束外形结构和油束特性参数。计算结果表明，高压涡旋喷油器油束边缘有环涡存在；随着喷射压力的增加燃油薄膜厚度减小，速度和油束贯穿度增加，轴截面索特平均直径减小；随着背压的升高，油束贯穿度减小而整体索特平均直径增加。该油束模型与实验结果吻合良好。     

房屋增层事故原因及预防措施

房屋增层是近年我国各城市为提高房屋利用所采用的一种行之有效的方法，并且已取得了很好的经济效益，文章中列举了国内的一些房屋增层所出现的事故，并对其作出分析，最后又提出了预防事故发生的措施。     

三峡库区忠县火电厂库岸边坡综合整治工程

三峡水库蓄水后，库岸坡再造必将带来的坡面稳定性问题。本文通过综合分析了忠县火电厂沿江库岸边坡的地形地质条件及其运行环境情况，提出了边坡治理的基本内容，采用对坡体稳定性分区和边坡运行条件分区，对不同地带的边坡提出不同的综合治理措施。