全文获取类型
收费全文 | 7094篇 |
免费 | 115篇 |
国内免费 | 205篇 |
专业分类
系统科学 | 127篇 |
丛书文集 | 164篇 |
教育与普及 | 125篇 |
理论与方法论 | 13篇 |
现状及发展 | 1975篇 |
研究方法 | 215篇 |
综合类 | 4783篇 |
自然研究 | 12篇 |
出版年
2015年 | 71篇 |
2014年 | 72篇 |
2013年 | 77篇 |
2012年 | 140篇 |
2011年 | 189篇 |
2010年 | 149篇 |
2009年 | 231篇 |
2008年 | 211篇 |
2007年 | 236篇 |
2006年 | 167篇 |
2005年 | 209篇 |
2004年 | 198篇 |
2003年 | 151篇 |
2002年 | 162篇 |
2001年 | 291篇 |
2000年 | 275篇 |
1999年 | 306篇 |
1998年 | 170篇 |
1997年 | 201篇 |
1996年 | 163篇 |
1995年 | 136篇 |
1994年 | 127篇 |
1993年 | 103篇 |
1992年 | 166篇 |
1991年 | 166篇 |
1990年 | 152篇 |
1989年 | 127篇 |
1988年 | 134篇 |
1987年 | 117篇 |
1986年 | 76篇 |
1985年 | 106篇 |
1984年 | 81篇 |
1983年 | 63篇 |
1982年 | 65篇 |
1981年 | 60篇 |
1980年 | 75篇 |
1979年 | 146篇 |
1978年 | 134篇 |
1977年 | 107篇 |
1976年 | 79篇 |
1975年 | 73篇 |
1974年 | 98篇 |
1973年 | 103篇 |
1972年 | 89篇 |
1971年 | 111篇 |
1970年 | 100篇 |
1969年 | 96篇 |
1968年 | 95篇 |
1967年 | 87篇 |
1966年 | 86篇 |
排序方式: 共有7414条查询结果,搜索用时 31 毫秒
11.
介绍了国内第一个综采放顶煤开采矿区铁路线下压煤试验面的成功经验,总结了试采中对铁路所采取的各种分析和维护措施,分析了该采煤方法下的地在移动和变形特点,为我国“三下”采煤领域提供了具有特色的成功实例。 相似文献
12.
The aim of this study is to identify the brain activity associated with amphetamine craving for understanding the neurobiology of addiction by watching pictures of happy, sad or amphetamine. Brain activity was measured in 12 amphetamine addicts and 12 healthy subjects by fMRI while the subjects watched pictures designed to elicit happy feelings, sad feelings, or the desire to use amphetamine. The result demonstrated that amphetamine cues led to abnormally high cingulate and low frontal lobe activation in amphetamine addicts, who showed abnormalities in affect-related brain activation. 相似文献
13.
Minagawa H Yoshida Y Kenmochi N Furuichi M Shimada J Kaneko H 《Cellular and molecular life sciences : CMLS》2007,64(1):77-81
Lactate oxidase is used in biosensors to measure the concentration of lactate in the blood and other body fluids. Increasing
the thermostability of lactate oxidase can significantly prolong the lifetime of these biosensors. We have previously obtained
a variant of lactate oxidase from Aerococcus viridans with two mutations (E160G/V198I) that is significantly more thermostable than the wild-type enzyme. Here we have attempted
to further improve the thermostability of E160G/V198I lactate oxidase using directed evolution. We made a mutant lactate oxidase
gene library by applying error-prone PCR and DNA shuffling, and screened for thermostable mutant lactate oxidase using a plate-based
assay. After three rounds of screening we obtained a thermostable mutant lactate oxidase, which has six mutations (E160G/V198I/G36S/T103S/A232S/F277Y).
The half-life of this lactate oxidase at 70 °C was about 2 times that of E160G/V198I and about 36 times that of the wild-type
enzyme. The amino acid mutation process suggests that the combined neutral mutations are important in protein evolution.
Received 15 September 2006; received after revision 21 October 2006; accepted 2 November 2006 相似文献
14.
Kang J.Y. Xu F.C. 《中国科学基金(英文版)》2004,(2):57-58,64
1 Introduction
Nanoscale science and technology have attracted greatattention since the novel properties become dominant for well-known materials as their sizes reduced to some critical dimension. These properties frequently originate in lattice distortions, structure transformations etc[1]. The variations of size and structure ofnanocrystals lead to the change of their electronic structures. Very recently, nanoscale materials with different electronic structures were utilized in fabricating nanoscale junctions[2].
…… 相似文献
15.
It is well known that transmitter secretion requires specialized secretory organelles, the synaptic vesicles, for the packaging, storage and exocytotic release of the transmitter. Here we report that when acetylcholine (ACh) is loaded into an isolated Xenopus myocyte, there is spontaneous quantal release of ACh from the myocyte which results in activation of its own surface ACh channels and the appearance of membrane currents resembling miniature endplate currents. This myocyte secretion probably reflects Ca(2+)-regulated exocytosis of ACh-filled cytoplasmic compartments. Furthermore, step depolarization of the myocyte membrane triggers evoked ACh release from the myocyte with a weak excitation-secretion coupling. These findings suggest that quantal transmitter secretion does not require secretory pathways unique to neurons and that the essence of presynaptic differentiation may reside in the provision of transmitter supply and modification of the preexisting secretion pathway. 相似文献
16.
17.
A. F. McDonagh Y. -M. Pu D. A. Lightner 《Cellular and molecular life sciences : CMLS》1992,48(3):246-248
The characteristic circular dichroism of bilirubin bound to human serum albumin undergoes a remarkable sign inversion on addition of halothane, chloroform and other volatile anesthetics. This sign inversion, which is completely reversed by removal of the anesthetic, reflects a pronounced conformational change of the bound ligand; probably a complete inversion of chirality. The observation suggests that association of volatile anesthetics with proteins can markedly alter the internal topography of receptor sites and potentially influence the stereoselectivity of ligand binding. 相似文献
18.
本文应用微量元素地球化学理论,通过研究区内地层、红土剖面及矿物相中微量元素含量分配特点,结合野外观察结果,得出了本区三水铝土矿的成矿源岩是郁江组地层的结论,为本区找矿提供了新的线索。 相似文献
19.
对缺乏锌、硼、钼、锰、铜等微量元素的葡萄叶细胞进行观察表明:细胞核结构异常,叶绿体外被膜不完整,片层膜系统模糊不清,较则排列杂乱,重则趋于瓦解、消失,基质中出现了数量不等的液泡. 相似文献
20.
在抽象经济的均衡存在定理中,一般都假设偏好对应具有开图像。用一个较弱的条件代替关于偏好对应的开图像假设,证明了均衡的存在性。所得结果是Yannelis(1987)均衡存在定理的一个推广. 相似文献