沿空留巷技术实践与研究

介绍了河北省邯郸市牛儿庄采矿有限公司在56803工作面回采期间沿空留巷的工业性实践,提出了具体的沿空留巷技术方案,通过回采期间沿空留巷巷道围岩变形的矿压观测,总结出沿空留巷的矿压显现规律,为进一步优化巷旁支护、实现无煤柱开采、提高煤炭回收率、缓解矿井采掘接续矛盾提供了实践依据.     

区间直觉纯语言信息的集结方法及其在群决策中的应用

对区间直觉纯语言信息的集结方法进行了研究.定义了区间直觉纯语言集及其运算法则和区间直觉纯语言变量的得分函数和精确函数,给出了一种简单的区间直觉纯语言变量的排序方法.进一步,提出了一些基于区间直觉纯语言信息及其运算法则的信息集结算子.在此基础上,给出了一种专家权重、属性权重及属性值均以语言标度形式给出的区间直觉纯语言信息集结方法,并将此方法应用到多属性群决策中.最后通过实例分析表明了该方法的有效性和可行性.     

核电厂环境风险评价框架及方法

 目前核电厂风险评价技术分为核事故风险评价及非人类物种电离辐射防护评价。为发展一个包括非人类物种防护在内的核电厂辐射防护体系,本文借鉴环境风险评价的关键流程要素,提出包括公众健康和非人类物种的核电厂环境风险评价框架。在这一框架的危害排序环节,对所选择的各评价终点指标采用层次分析法,计算评价终点对核电厂环境风险的权重并进行排序,旨在发现对环境风险贡献较大的评价终点并在风险管理中对其优先管理控制。     

High Level Expression of tcs Gene in E.coli

ＨｉｇｈＬｅｖｅｌＥｘｐｒｅｓｓｉｏｎｏｆｔｃｓＧｅｎｅｉｎＥ．ｃｏｌｉ＊ＬｉｕＹｕｅ（刘月），ＺｈｕＣｈｕｎｍｉｎｇ（朱春明），ＹｅＺｈａｏｈｕｉ（叶朝晖），ＬｉｕＪｉｎｙｕａｎ（刘进元），ＺｈａｎｇＲｉｑｉｎｇ（张日清）ＤｅｐａｒｔｍｅｎｔｏｆＢ...     

圆柱绕流N一S方程数值解的非线性特性分析

数值模拟并分析了二维圆柱绕流向混沌转捩的情形．通过时间延迟技术和维数嵌入方法对速度时间序列重构相空间，并利用蛎线性动力系统理论方法（诸如相空分析、频谱分析、Ｐｏｉｎｃａｒｅ映射），以及通过对混沌计量（如关联维数、Ｋｏｌｍｏｇｒｏｖ熵、Ｌｙａｐｕｎｏｖ指数等）的计算，对不同雷诺数的Ｎ－Ｓ方程解的特征作了分辨．结果表明，在一定的雷诺数范围内解的是周期或周期的，并且有一个复杂的公叉途径向混沌转捩．     

A numerical study on nonlinear propagation and short-term variability of the migrating diurnal and semidiurnal tides

By using a three-dimensional fully nonlinear numerical model in spherical coordinates and taking the linear steady solutions of the migrating diurnal and semidiurnal tides in January from the Global-Scale Wave Model （GSWM） as the initial values, we simulate the linear and nonlinear propagations of the migrating diurnal and semidiurnal tides in the atmosphere from the ground to the lower thermosphere. A comparison of our simulations with the results of GSWM is also presented. The simulation results show that affected by the nonlinearity, the migrating diurnal and semidiurnal tides propagating in the middle and upper atmosphere exhibit evident short-term variability. The nonlinear interactions between the migrating tides and the background atmosphere can obviously alter the background wind and temperature fields, which suggests that the nonlinear propagations of the migrating diurnal and semidiurnal tides impact significantly on the transient dynamical and thermal structures of the background middle and upper atmosphere and the nonlinear effect is an important cause of the difference between the results of GSWM and observations.     

Progress in interspecies cloning of mammals

Interspecies mammalian cloning can be achieved by application of two key techniques, i.e.the technique of interspecies nuclear transfer and the technique of interspecies pregnancy.The general principles, problems and possible solutions, as well as the recent advances of interspecies mammalian cloning have been summarized in this review.     

旧水泥混凝土路面大修整治技术的讨论分析

分析水泥混凝土公路路面中存在的问题,在此基础上,提出修整改造措施,对施工过程进行详细说明。     

Combined immunity of DNA vector and recombinant vaccinia virus expressing Gag proteins of equine infectious anemia virus

In order to develop a new vaccine candidate for equine infectious anemia virus (EIAV), gag gene of Chinese donkey leukocyte attenuated strain (EIAV DLV) and its parental virulent strain (EIAV LN) were inserted respectively into the TK region of the Tiantan strain (VV) of vaccinia virus by homologous recombination and the positive clone was confirmed by blue plaque assay. Protein expression was examined by Western blot. Prime and prime-boost procedures were used to immunize mice with two DNA vectors and two recombinant vaccinia viruses expressing EIAV Gag proteins. The results showed that the specific lysis of CTL responses in the DNA rVV groups was stronger than those in the DNA groups, amounting to 31%. Although the levels of specific antibodies were not significantly different, we could conclude that the recombinant vaccinia virus could boost the cellular responses following DNA vector priming. There was no detectable difference between the immune responses induced by DLV and LN Gag proteins. This data demonstrates that the combined immunity of DNA vector and recombinant vaccinia virus expressing EIAV gag proteins, utilizing the prime-boost procedure, can drive immunized mice to produce powerful cellular responses. These results lay an important foundation for the development of a new EIAV genetic engineering vaccine.     

微波辅助双水相提取苦荞麦粉中黄酮类化合物

采用微波辅助乙醇-硫酸铵双水相体系,从苦荞麦粉中提取黄酮类化合物.确定乙醇-硫酸铵双水相提取黄酮类化合物的较优乙醇体积分数以及硫酸铵添加量;通过单因素实验确定苦荞麦粉黄酮类化合物的较优萃取条件.结果表明:微波辅助双水相的方法适合用于苦荞麦粉中黄酮类化合物的提取;响应面法优化微波辅助双水相提取苦荞麦粉黄酮的提取条件,较优提取条件为萃取剂含乙醇的体积分数30%,微波功率550 W,提取时间70 s,苦荞麦粉在提取溶剂中的质量浓度0.02 g/m L,苦荞麦粉中提取得到的黄酮类化合物占苦荞麦粉的质量分数为1.38%.