Metachromasia in cultured fibroblasts of subjects with glycogenosis type II

Riassunto Frammenti di cute di due soggetti affetti da glicogenosi tipo II e dei loro genitori e fratelli sono stati coltivati in vitro. Sia nelle culture dei pazienti che dei loro familiari si è osservata la presenza di materiale metacromatico dopo colorazione con blu di toluidina 0. Nei leucociti di tutti i familiari esaminati l'attività della -1,4-glucosidasi è risultata ridotta.     

Auxin dependence and auxin oxidase of cultured sycamore cells

Summary Quantitative and qualitative differences in auxin-oxidases extracted from auxin-dependent (S) or auxin-independent (MB) sycamore cells were analyzed. MB auxin-oxidases have a higher activity, but the molecular weight of this enzymatic complex is lowered by freeze-drying, without loss of the activity. Correlations with auxin-independence are discussed in this context.     

A simple filtration device to study the interaction of RNA-polymerase with DNA

Summary An accessory for a commercial 10-place filtration apparatus is described, which allows the easy recovery of both filter-bound material and flow-through in the study of DNA-protein interactions by assaying binding of protein to cellulose nitrate filters.The technical assistance of Christine Michalowski is gratefully acknowledged., Supported by the Deutsche Forschungsgemeinschaft.     

(E,E)-10,12-Hexadecadienal: A component of the female sex pheromone of the spiny bollworm,Earias insulana (Boisd.) (Lepidoptera: Noctuidae)

Summary (E,E)-10,12-Hexadecadienal has been identified as a component of the sex pheromone of the female spiny bollworm moth,Earias insulana, by gas-chromatographic, electroantennographic and microchemical studies of abdominal tip extracts and entrained volatiles from female moths.This work was carried out under Ministry of Agriculture, Fisheries and Food licence HH 11599/139, issued under the Destructive Pests and Diseases of Plants Order 1965.