全文获取类型
收费全文 | 366篇 |
免费 | 2篇 |
国内免费 | 1篇 |
专业分类
系统科学 | 6篇 |
丛书文集 | 1篇 |
理论与方法论 | 1篇 |
现状及发展 | 38篇 |
研究方法 | 64篇 |
综合类 | 234篇 |
自然研究 | 25篇 |
出版年
2021年 | 1篇 |
2020年 | 1篇 |
2017年 | 4篇 |
2016年 | 4篇 |
2015年 | 3篇 |
2014年 | 8篇 |
2013年 | 5篇 |
2012年 | 40篇 |
2011年 | 60篇 |
2010年 | 10篇 |
2009年 | 1篇 |
2008年 | 33篇 |
2007年 | 43篇 |
2006年 | 37篇 |
2005年 | 26篇 |
2004年 | 25篇 |
2003年 | 23篇 |
2002年 | 31篇 |
2000年 | 1篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1994年 | 1篇 |
1992年 | 1篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1976年 | 1篇 |
排序方式: 共有369条查询结果,搜索用时 31 毫秒
191.
A PHD finger of NURF couples histone H3 lysine 4 trimethylation with chromatin remodelling 总被引:1,自引:0,他引:1
192.
193.
Mantle wedge control on back-arc crustal accretion 总被引:1,自引:0,他引:1
At mid-ocean ridges, plate separation leads to upward advection and pressure-release partial melting of fertile mantle material; the melt is then extracted to the spreading centre and the residual depleted mantle flows horizontally away. In back-arc basins, the subducting slab is an important control on the pattern of mantle advection and melt extraction, as well as on compositional and fluid gradients. Modelling studies predict significant mantle wedge effects on back-arc spreading processes. Here we show that various spreading centres in the Lau back-arc basin exhibit enhanced, diminished or normal magma supply, which correlates with distance from the arc volcanic front but not with spreading rate. To explain this correlation we propose that depleted upper-mantle material, generated by melt extraction in the mantle wedge, is overturned and re-introduced beneath the back-arc basin by subduction-induced corner flow. The spreading centres experience enhanced melt delivery near the volcanic front, diminished melting within the overturned depleted mantle farther from the corner and normal melting conditions in undepleted mantle farther away. Our model explains fundamental differences in crustal accretion variables between back-arc and mid-ocean settings. 相似文献
194.
Selker EU Tountas NA Cross SH Margolin BS Murphy JG Bird AP Freitag M 《Nature》2003,422(6934):893-897
Cytosine methylation is common, but not ubiquitous, in eukaryotes. Mammals and the fungus Neurospora crassa have about 2-3% of cytosines methylated. In mammals, methylation is almost exclusively in the under-represented CpG dinucleotides, and most CpGs are methylated whereas in Neurospora, methylation is not preferentially in CpG dinucleotides and the bulk of the genome is unmethylated. DNA methylation is essential in mammals but is dispensable in Neurospora, making this simple eukaryote a favoured organism in which to study methylation. Recent studies indicate that DNA methylation in Neurospora depends on one DNA methyltransferase, DIM-2 (ref. 6), directed by a histone H3 methyltransferase, DIM-5 (ref. 7), but little is known about its cellular and evolutionary functions. As only four methylated sequences have been reported previously in N. crassa, we used methyl-binding-domain agarose chromatography to isolate the methylated component of the genome. DNA sequence analysis shows that the methylated component of the genome consists almost exclusively of relics of transposons that were subject to repeat-induced point mutation--a genome defence system that mutates duplicated sequences. 相似文献
195.
X-ray structure of a voltage-dependent K+ channel 总被引:24,自引:0,他引:24
Voltage-dependent K+ channels are members of the family of voltage-dependent cation (K+, Na+ and Ca2+) channels that open and allow ion conduction in response to changes in cell membrane voltage. This form of gating underlies the generation of nerve and muscle action potentials, among other processes. Here we present the structure of KvAP, a voltage-dependent K+ channel from Aeropyrum pernix. We have determined a crystal structure of the full-length channel at a resolution of 3.2 A, and of the isolated voltage-sensor domain at 1.9 A, both in complex with monoclonal Fab fragments. The channel contains a central ion-conduction pore surrounded by voltage sensors, which form what we call 'voltage-sensor paddles'-hydrophobic, cationic, helix-turn-helix structures on the channel's outer perimeter. Flexible hinges suggest that the voltage-sensor paddles move in response to membrane voltage changes, carrying their positive charge across the membrane. 相似文献
196.
The hydrodynamics of water strider locomotion 总被引:3,自引:0,他引:3
Water striders Gerridae are insects of characteristic length 1 cm and weight 10 dynes that reside on the surface of ponds, rivers, and the open ocean. Their weight is supported by the surface tension force generated by curvature of the free surface, and they propel themselves by driving their central pair of hydrophobic legs in a sculling motion. Previous investigators have assumed that the hydrodynamic propulsion of the water strider relies on momentum transfer by surface waves. This assumption leads to Denny's paradox: infant water striders, whose legs are too slow to generate waves, should be incapable of propelling themselves along the surface. We here resolve this paradox through reporting the results of high-speed video and particle-tracking studies. Experiments reveal that the strider transfers momentum to the underlying fluid not primarily through capillary waves, but rather through hemispherical vortices shed by its driving legs. This insight guided us in constructing a self-contained mechanical water strider whose means of propulsion is analogous to that of its natural counterpart. 相似文献
197.
Deficiency of Mbd2 suppresses intestinal tumorigenesis 总被引:16,自引:0,他引:16
Gene silencing through de novo methylation of CpG island promoters contributes to cancer. We find that Mbd2, which recruits co-repressor complexes to methylated DNA, is essential for efficient tumorigenesis in the mouse intestine. As Mbd2-deficient mice are viable and fertile, their resistance to intestinal cancer may be of therapeutic relevance. 相似文献
198.
Curvature of clathrin-coated pits driven by epsin 总被引:33,自引:0,他引:33
Clathrin-mediated endocytosis involves cargo selection and membrane budding into vesicles with the aid of a protein coat. Formation of invaginated pits on the plasma membrane and subsequent budding of vesicles is an energetically demanding process that involves the cooperation of clathrin with many different proteins. Here we investigate the role of the brain-enriched protein epsin 1 in this process. Epsin is targeted to areas of endocytosis by binding the membrane lipid phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P(2)). We show here that epsin 1 directly modifies membrane curvature on binding to PtdIns(4,5)P(2) in conjunction with clathrin polymerization. We have discovered that formation of an amphipathic alpha-helix in epsin is coupled to PtdIns(4,5)P(2) binding. Mutation of residues on the hydrophobic region of this helix abolishes the ability to curve membranes. We propose that this helix is inserted into one leaflet of the lipid bilayer, inducing curvature. On lipid monolayers epsin alone is sufficient to facilitate the formation of clathrin-coated invaginations. 相似文献
199.
Percy KE Awmack CS Lindroth RL Kubiske ME Kopper BJ Isebrands JG Pregitzer KS Hendrey GR Dickson RE Zak DR Oksanen E Sober J Harrington R Karnosky DF 《Nature》2002,420(6914):403-407
Human activity causes increasing background concentrations of the greenhouse gases CO2 and O3. Increased levels of CO2 can be found in all terrestrial ecosystems. Damaging O3 concentrations currently occur over 29% of the world's temperate and subpolar forests but are predicted to affect fully 60% by 2100 (ref. 3). Although individual effects of CO2 and O3 on vegetation have been widely investigated, very little is known about their interaction, and long-term studies on mature trees and higher trophic levels are extremely rare. Here we present evidence from the most widely distributed North American tree species, Populus tremuloides, showing that CO2 and O3, singly and in combination, affected productivity, physical and chemical leaf defences and, because of changes in plant quality, insect and disease populations. Our data show that feedbacks to plant growth from changes induced by CO2 and O3 in plant quality and pest performance are likely. Assessments of global change effects on forest ecosystems must therefore consider the interacting effects of CO2 and O3 on plant performance, as well as the implications of increased pest activity. 相似文献
200.
The performance degradation of GaAs multi quantum well solar cells (MQWSCs) has been studied using 2 MeV proton irradiation with fluences from 3×109 to 2×1013 p/cm2. The results have shown that the spectral response of MQWSCs clearly reduces in the short wavelength side after the proton irradiation,and the experimentally determined absolute ΔVoc loss was found to be a good match to a model which describes the absolute ΔVoc. The cells are seen to display good irradiation tolerance for the degradation rate of the Pmax under the irradiation with a high fluence of 3×1011 p/cm2 being only 25%. 相似文献