Symmetrical directional cloning: An efficient method to prepare hairpin RNA interference constructs

RNA interference (RNAi) is a loss-of-function approach by which double-stranded RNA (dsRNA) initiates degradation of homologous mRNAs in a sequence specific manner. The dsRNA molecules can be produced in vitro or in vivo, and can be introduced to cells in a number of ways. Here we report a more efficient method for the cloning of inverted repeat DNA fragments into expression vectors that can be transcribed into effective dsRNA molecules in vivo or in vitro. This method, named Symmetrical Directional Cloning (SDC), takes the advantage of compatible non-palindromic restriction enezyme sites, which allow one to directionally clone a single PCR product in both the sense and antisense orientations together into a vector. SDC allows for the directional cloning of inverted repeats using a single PCR product; it requires only one cut site on each side of the loop. Hence this method is more cost effective and less time-consuming. At least 21 commercially available restriction endonucleases can be used as cloning sites for the SDC method. The efficacy of dsRNA expression vectors prepared by SDC has been demonstrated by targeting a negative regulator of the signaling pathway mediating the response of cells to phytohormone, gibberellins (GA), in the aleurone cells.     

NPP distribution related to the terrains along the North-South Transect of Eastern China

Vegetation net primary productivity (NPP) is defined as the accumulative amount of organic matters per unit area and time, which is calculated from the difference between accumulative photosynthesis and accumulative autotrophic respiration by green plants. Since the NPP reflects the physiological and ecological characteristics of the plants and the interactive results of the environmental factors, it is an important index to evaluate a structure of ecological system, functional features and …     

Simulation of meridional overturning in the upper layer of the South China Sea with an idealized bottom topography

The South China Sea (SCS) is the largest marginal sea in the northwestern Pacific. Several important water gateways exist around the SCS, such as Luzon Strait, Taiwan Strait, etc., that lie in the northeastern SCS, and others like Kalimantan Strait, Balawan Strait, etc., lie in the south. Luzon Strait connects the SCS and Pacific via the Philippine Sea, and its water exchange has significant impact on the interior circulations of the SCS[1]. Since direct observation data about the …     

Detection of the translational oscillations of the Earth''''s solid inner core based on the international superconducting gravimeter observations

Before about 100 years, the layered structures of the Earths body and the existence of the liquid core had been found, then the solid inner core and detailed structures in the interior of the Earth are demonstrated, it proves that the satisfied achievements had been obtained in recogni-tion of the Earths interior structures. In recent years, with the increasing requirements of the basic sciences and space techniques to geodynamics of the Earths interior, the theoretical study and practical de…     

Simple scaling relations in geodynamics： the role of pressure in mantle convection and plume formation

Scaling relations are important in extrapolating laboratory experiments to the Earth‘s mantle. In planetary interiors, compression becomes an important parameter and it is useful to explore scalings that involve volume. I use simple volume scaling relations that allow one to extrapolate laboratory experiments and upper mantle behavior, in a thermodynamically self-consistent way, to predict lower mantle behavior. The relations are similar to the quasi-har-monic approximation. Slabs and plates have characteristic dimensions of hundreds of kilometers and time constants of 100 million years, but the volume scalings predict order of magnitude higher values in the deep mantle. The scaling relations imply that the deep mantle is a sluggish system with ancient features. They imply irreversible chemical stratification and do not favor the plume hypothesis.     

Providing QoS guarantees for self-similar traffic flows

1.INTRODUCTION Recentanalysesofvariouspacket basednetworking showthatnetworktraffichasvariabilityovermany timescales.Itsperformancedifferswithresultsof queuetheorythatisbasedonthePoissondistribu tion.Statisticalanalysesoflargetrafficdatasetshave revealedthatthestatisticalcharacteristicsofrealtraf ficmaybebestdescribedbyself similarity.Self simi larityimpliesthepresenceoflong rangedependence (LRD)inthetraffic.Thatis,thereexistnon negli giblepositivecorrelationintheburstbehaviorofthe …     

Use of piecewise polynomial phase modeling to compensate ionospheric phase contamination in skywave radar systems

1.INTRODUCTION High frequencyskywaveover the horizonradarcan providearange coverageofupto4000kmbymeans oftherefractionwithintheionosphere.Butthesignal contaminationsufferedindoubleionospherictransit, especially,thenonstationarityoftheionosphereren derstheechospectrumdistortedandresolutionofco herentintegrationtechniquethatiswidelyappliedin theradarsystemsisdegradedextremely. InsomeapplicationsofHFskywaveradar,some complexgeophysicalmechanismsincludedthevaria tionoftheionospheremaypro…     

ARMA-GM combined forewarning model for the quality control

1.INTRODUCTION Tocontrolthequalityoftheproducts,wemustsu pervisethequalityindexesandanalyzethechanging tendency.Therearealotofstatisticaltoolsincom monuse,themosteffectivemethodisacontrol chart.Itcangrasptheabnormalsymptomofatrend ofthequalityofproductsincontrollingprocessand makethequalitypreventionpossible.Butfromits workingprocessweknowthismethodalsohassome limitation.Becausethecontrolchartonlydetermines theaveragevalueandlimitinterval,onecanonly judgethequalitystateofproductsstatica…     

New structure of Kalman filter for radar networking

1.INTRODUCTION Inpractice,radarsoftenoperateasynchronouslyandpro videdataatdifferentrateswithdifferentcommunication timedelays,thustheproblemofdatafusioninradarnet workingsystemisasynchronousinsteadofsynchronous. However,theresearchinthefieldofdatafusionfocuses onthesynchronousproblems,andthemeasurementsof differentsensorsarealwayspredictedatthesametimefor updatingKalmanfiltersynchronously.Thisapproachis complicatedforengineering,anditwillreducethedata ratesofthesystem,atthesametimethee…     

Establishing formal state space models via quantization forquantum control systems

1.INTRODUCTION Quantumtheoryisoneofthemostimportantachieve mentsinthe20thcentury,anditdisclosesthestruc tures,characteristicsandmotionlawsofmatterinthe microcosmicfieldandleadsusfromthemacroscopical worldintomicrosystems.Aseriesofnewphenomena whicharedifferentfromclassicalsystemshavebeen discovered,suchasquantumentanglement,quantum coherenceanduncertainty.Meanwhile,quantum theoryandquantummethodsarealsowidelyusedin differentfieldssuchaschemicalreaction,geneticen gineering,atomicphysicsandi…