A radiation hybrid map of mouse genes

A comprehensive gene-based map of a genome is a powerful tool for genetic studies and is especially useful for the positional cloning and positional candidate approaches. The availability of gene maps for multiple organisms provides the foundation for detailed conserved-orthology maps showing the correspondence between conserved genomic segments. These maps make it possible to use cross-species information in gene hunts and shed light on the evolutionary forces that shape the genome. Here we report a radiation hybrid map of mouse genes, a combined project of the Whitehead Institute/Massachusetts Institute of Technology Center for Genome Research, the Medical Research Council UK Mouse Genome Centre, and the National Center for Biotechnology Information. The map contains 11,109 genes, screened against the T31 RH panel and positioned relative to a reference map containing 2,280 mouse genetic markers. It includes 3,658 genes homologous to the human genome sequence and provides a framework for overlaying the human genome sequence to the mouse and for sequencing the mouse genome.     

Blocking of lectin-like adhesion molecules on pulmonary cells inhibits lung sarcoma L-1 colonization in BALB/c-mice

Summary Adhesion and inhibition experiments with pulmonary cells of BALB/c-mouse origin and syngeneic sarcoma L-1 cells indicated that L-fucose specific lectin-like adhesion molecules, presumably situated on pulmonary cell surfaces are (at least partly) responsible for the specificity of this cell-cell interaction. Addition of specific sugars and glycoconjugates (L-fucose and fucoidan, respectively) to the incubation medium evidently inhibited the adhesion process as quantified using radiolabelled tumor cells. Unspecific carbohydrates (e.g. D-galactose) did not affect the cellular interaction. In vivo, repeated administration of fucoidan (but not of unspecific glycoconjugates) significantly inhibited the settling of metastatic sarcoma L-1 cells in the lungs of BALB/c-mice. Therefore, when lectin-like adhesion molecules on pulmonary cells were blocked with competitive glycoconjugates, tumor cell colonization of the lung could be significantly inhibited.     

Cross-sectional approach for clustering time varying data

Cluster analysis is to be performed on a three-mode data matrix of type: units, variables, time. A general model for calculating the distance between two units varying in time is proposed. One particular model is developed and used in an example concerned with clustering of 23 European countries according to the similarity of energy consumption in the years 1976–1982.Supported in part by the Research Council of Slovenia, Yugoslavia.     

Experimental studies on embryonic development ofDendrocoelum lacteum O. F. Müller

Résumé En culture entre lamelles de verre, on a examiné expérimentalement le développement embryonnaire deDendrocoelum lacteum et observé le phénomène de la polyembryonie expérimentale analogue à la polyembryonie naturelle.     

Aktivität einiger Transaminasen in den mit SO2 behandelten Erbsen-Keimpflanzen

Summary Fifteen-day-old green pea seedlings were intoxicated 48 h by 1% gaseous sulphur dioxide and in their individual organs the activity of glutamate-oxalacetate (l-aspartate: 2-ketoglutarate-aminotransferase) and aspartate-pyruvate (l-alanine: oxalacetate-aminotransferase) transaminases estimated. The activity of glutamate-oxalacetate transaminase in the intoxicated seedlings was lowered in shoots by 77.8% and in roots by 78,9% as compared with the control plants. The activity of aspartate-pyruvate transaminase was lowered in shoots by 33.5%, in roots by 76% and in cotyledons by 54.5% in comparison with the control plants.     

Fractionation of highly purified bovine growth hormone on sephadex G-25 gel

Zusammenfassung Hochgereinigtes Rinderwachstumshormon wurde durch Sephadex G-25 Gel-Chromatographie in zwei Komponenten zerlegt. Beide Fraktionen wie auch das unfraktionierte Präparat erwiesen sich in bezug auf Aminosäurenzusammensetzung, Elektrophorese und biologische Aktivität identisch.     

Sr2+ can become incorporated into an agonist-sensitive,cytoplasmic Ca2+ store in a cell line derived from the equine sweat gland epithelium

We have explored the properties of a Ca²⁺-dependent cell-signalling pathway that becomes active when cultured equine sweat gland cells are stimulated with ATP. The ATP-regulated, Ca²⁺-influx pathway allowed Sr²⁺ to enter the cytoplasm but permitted only a minimal influx of Ba²⁺. Experiments in which cells were repeatedly stimulated with ATP suggested that Sr²⁺, but not Ba²⁺, could become incorporated into the agonist-sensitive, cytoplasmic Ca²⁺ store. Further evidence for this was provided by experiments using ionomycin, a Ca²⁺ ionophore which has no affinity for Sr²⁺.