Sequence of an unusually large protein implicated in regulation of myosin activity in C. elegans

The Caenorhabditis elegans gene unc-22 encodes a very large muscle protein, called twitchin, which consists of a protein kinase domain and several copies of two short motifs. The sequence of twitchin has unexpected similarities to the sequences of proteins of the immunoglobulin superfamily, cell adhesion molecules and vertebrate muscle proteins, including myosin light-chain kinase. These homologies, together with results from earlier genetic and molecular analyses, indicate that twitchin is involved in a novel mechanism of myosin regulation.     

Genetic imprinting suggested by maternal heterodisomy in nondeletion Prader-Willi syndrome

Prader-Willi syndrome (PWS) is the most common form of dysmorphic genetic obesity associated with mental retardation. About 60% of cases have a cytological deletion of chromosome 15q11q13 (refs 2, 3). These deletions occur de novo exclusively on the paternal chromosome. By contrast, Angelman syndrome (AS) is a very different clinical disorder and is also associated with deletions of region 15q11q13 (refs 6-8), indistinguishable from those in PWS except that they occur de novo on the maternal chromosome. The parental origin of the affected chromosomes 15 in these disorders could, therefore, be a contributory factor in determining their clinical phenotypes. We have now used cloned DNA markers specific for the 15q11q13 subregion to determine the parental origin of chromosome 15 in PWS individuals not having cytogenetic deletions; these individuals account for almost all of the remaining 40% of PWS cases. Probands in two families displayed maternal uniparental disomy for chromosome 15q11q13. This is the first demonstration that maternal heterodisomy--the presence of two different chromosome 15s derived from the mother--can be associated with a human genetic disease. The absence of a paternal contribution of genes in region 15q11q13, as found in PWS deletion cases, rather than a mutation in a specific gene(s) in this region may result in expression of the clinical phenotype. Thus, we conclude that a gene or genes in region 15q11q13 must be inherited from each parent for normal human development.     

Functional cloning of ICAM-2, a cell adhesion ligand for LFA-1 homologous to ICAM-1

The leukocyte adhesion molecule LFA-1 mediates a wide range of lymphocyte, monocyte, natural killer cell, and granulocyte interactions with other cells in immunity and inflammation. LFA-1 (CD11a/CD18) is a receptor for intercellular adhesion molecule 1 (ICAM-1, CD54), a surface molecule which is constitutively expressed on some tissues and induced on other in inflammation. Induction of ICAM-1 on epithelial cells, endothelial cells and fibroblasts mediates LFA-1-dependent adhesion of lymphocytes. Several lines of evidence have suggested the existence of a second LFA-1 ligand: homotypic adhesion of one cell line was inhibited by a monoclonal antibody to LFA-1, but not by one to ICAM-1; there exists an LFA-1-dependent, ICAM-1-independent pathway of adhesion to endothelial cells; and also, there are some types of target cells in which LFA-1-dependent T-lymphocyte adhesion and lysis are independent of ICAM-1. We have cloned this second ligand, designated ICAM-2, using a novel method for identifying ligands of adhesion molecules. ICAM-2 is an integral membrane protein with two immunoglobulin-like domains, whereas ICAM-1 has five. Remarkably, ICAM-2 is much more closely related to the two most N-terminal domains of ICAM-1 (34% identity) than either ICAM-1 or ICAM-2 is to other members of the immunoglobulin superfamily, demonstrating the existence of a subfamily of immunoglobulin-like ligands that bind the same integrin receptor.     

Sφ（f）谱密度分析在可编程计数器上的实现

本文简述频率稳定度在频域中的表征和用Hadmard 方差进行相位噪声谱密度S_φ(f)分析原理.从而提出用可编程计数器进行相位噪声谱密度测量分析和计算的方法.     

梯形桁梁桥侧倾稳定分析的一种有限元法

本文将梯形桁梁桥的斜桥门架节间视作一子结构,通过其非满秩的失稳模态矩阵对子结构的刚度阵和几何刚度阵进行线性变换,得到子结构略去高阶失稳模态座标下的刚度阵和几何刚度阵;对于梯形桁梁桥的中间桁梁段则用桁梁梁段单元进行处理。这样,通过斜桥门架稳定子结构的广义位移与相邻梁段单元的广义位移之间的几何关系,即可得到计算梯形桁梁桥侧倾稳定的子结构与梁段单元相结合的有限元方法。本文给出了算例,并将结果与其它方法的结果进行了比较。结果表明,该方法具有很好的精度。     

具有非线性边条连续梁的动态建模

本文提出一种建立具有非线性边条连续梁动力学模型的方法。该方法侧重研究:(1)非线性边条的分段线性替代;(2)速度不连续模型的建立;(3)不同边条下广义力和初始条件的确定方法。该方法对研究压气机舌形阀片这类问题的动态响应具有重要的参考价值。     

体育教学中技评成绩的模糊综合评判

在体育教学中，运动技术成绩的评定方法存在许多不足，运用模糊综合评判的方法，可为各项运动技术成绩的评定建立多级模糊综合评判的数学模型，因而是一种比较好的评定运动技术成绩的新方法。     

海南万宁礼纪青梅林（续完）

海南万宁礼纪青梅林（续完）梁淑群，林英，杨小波，黄世满，符史新，谢国干（海南大学）礼纪青皮林自然保护区植物名录面类植物门ＰＴＥＲＩＤＯＰＨＹＴＡ１松叶蕨科Ｐｓｉｌｏｔａｃｅａｅ１）松叶蕨Ｐｓｉｌｏｔｕｍｎｕｄｕｍ（Ｌｉｎｎ，）Ｇｒｉｓｅｂ２水龙骨科Ｐ...     

量子Monte Carlo固定节面法对分子的研究：—一种新的试探函数

用量子Ｍｏｎｔｅ Ｃａｒｌｏ固定节面法计算了ＣＨ４和Ｈ２Ｏ分子的基态能，获得了９２．４％和７３．８％的相关能，计算中使用了一种新的试探函数，它满足电子与电子，电子与核的奇点条件。     

轿车车身表面压强分布的数值模拟

本文介绍了轿车身表面压强分布的数值模拟的理论依据及相应方法。建立了轿车车身外形数学模型和表面分布的物理模型，通过数值模拟结果与实验结果的对比，证明了该计算模型的可行性和正确性。