Genomic sequencing of colorectal adenocarcinomas identifies a recurrent VTI1A-TCF7L2 fusion

Prior studies have identified recurrent oncogenic mutations in colorectal adenocarcinoma and have surveyed exons of protein-coding genes for mutations in 11 affected individuals. Here we report whole-genome sequencing from nine individuals with colorectal cancer, including primary colorectal tumors and matched adjacent non-tumor tissues, at an average of 30.7× and 31.9× coverage, respectively. We identify an average of 75 somatic rearrangements per tumor, including complex networks of translocations between pairs of chromosomes. Eleven rearrangements encode predicted in-frame fusion proteins, including a fusion of VTI1A and TCF7L2 found in 3 out of 97 colorectal cancers. Although TCF7L2 encodes TCF4, which cooperates with β-catenin in colorectal carcinogenesis, the fusion lacks the TCF4 β-catenin-binding domain. We found a colorectal carcinoma cell line harboring the fusion gene to be dependent on VTI1A-TCF7L2 for anchorage-independent growth using RNA interference-mediated knockdown. This study shows previously unidentified levels of genomic rearrangements in colorectal carcinoma that can lead to essential gene fusions and other oncogenic events.     

Identification by mutagenesis of a Mg(2+)-block site of the NMDA receptor channel.

The N-methyl-D-aspartate (NMDA) receptor channel is highly permeable to Ca2+ but is blocked by Mg2+ in a voltage-dependent manner. These characteristics are essential for the NMDA receptor channel to mediate the induction of long-term potentiation of synaptic efficacy, a form of activity-dependent synaptic plasticity thought to underlie memory, learning and development. Recent studies have revealed the molecular and functional diversity of the NMDA receptor channel subunits, which are classified into the epsilon and zeta families according to the amino-acid sequence homology. Here we report that replacement by glutamine of asparagine 598 in putative transmembrane segment M2 of the zeta 1 subunit, strongly reduces the sensitivity of the heteromeric epsilon 2/zeta 1 NMDA receptor channel to Mg2+ block. The corresponding mutation of the epsilon 2 subunit has a similar effect. Furthermore, the heteromeric epsilon 2/zeta 1 NMDA receptor channel with the mutation on both subunits shows greatly reduced sensitivity to MK-801, a channel blocker of the NMDA receptor channel, but is still susceptible to inhibition by Zn2+. These findings suggest that the conserved asparagine residue in segment M2 constitutes a Mg(2+)-block site of the NMDA receptor channel, and that the MK-801 site overlaps the Mg2+ site.     

早熟,高配,抗逆玉米自交系“自改—1”的选育

“自改—1”玉米自交系是由“330×oh43HtHt—早”组合中经多代选株自交而成。“自改—1”自交系既保持了“330”自交系主要优良性状,又克服了“330”的缺点,并提高了抗病能力、缩短了生育期。是一个自身产量高、配合力好、早熟、抗病、抗倒、抗旱性强、农艺性状优良的理想自交系。“自改—1”玉米自交系具有较高的配合力。“自改—1×黄早四”杂交种(原名廊玉2号),已被河北省农作物品种审定委员会审定,定名为“冀单15号”。“8112×自改—1”、“1984×自改—1”参加了省区域试验。“自改—1×掖107"、“自改—1×23”等组合在多点试验评比中表现突出。这些组合在生产上具有较高的利用价值。     

框架结构大跨度预应力整体转换结构体系受力特点分析

以实际工程为背景，介绍了一种可以实现框架结构较大空间的新型预应力整体转换结构体系，并就该转换结构体系不同结构方案的受力特点以及预应力施加方案对转换结构体系内力的影响进行了较系统的计算分析比较，在此基础上提出了相应的设计建议。     

Application of Finite State Machine Theory to the Simulation of Reversed Non-Linear Hysteretic Relationships

This paper presents the application of finite state machine (FSM) theory to the programming of nonlinear hysteretic model simulation for both known and newly created rules. The complicated reversed internal paths involved in the nonlinear relationship which not only depend on material properties, but also on load history, often confuse rule creators and scholars. In this paper, we first describe the development of past hysteretic models. Then we introduce the FSM theory conceptually, and explain how it is applied to reversed and diverse routes. Next, state definitions and procedures are explained with a specific data example using the bilinear model. Finally, the successful application to UC-win/FRAME (3D) is described and several characteristics are summarized. By using FSM’s states and the linkages to represent a hysteresis model, we can quickly realize the programming of the defined complex model rules, and the nonlinear modeling becomes more efficient and feasible.     

纳米磁性膨润土复合材料表征及降解甲基橙研究

以纯化膨润土为原料,通过铁盐与膨润土浆液反应制备非均相纳米磁改性Fe3O4/膨润土复合材料,研究其对甲基橙的降解性能。结果表明,纳米磁性颗粒以Fe3O4附载于膨润土上形成磁性集合体,其对甲基橙废水等温吸附符合Langmuir等温吸附模型。在pH为7、温度为25℃、纳米磁性膨润土投加量为1g/L、H2O2投加量为2.5mmol/L、降解时间为120min条件下,对模拟甲基橙废水降解率达96%,CODCr去除率为94.7%,色度去除率为88.8%。     

具有脉冲的时滞Logistic方程的全局吸引性

考虑具有脉冲的时滞Logistic方程其中τ＞0,r(t)∈C([t0,+∞),R+);-1＜bk≤0;且{tk}满足t0＜t1＜t2＜…＜tk＜…,limtk=+∞,给出了(*)的解是全局吸引的充分条件.     

考虑初始投资递减的住宅能源系统的经济性评价

近年来，由于具有较高的能源利用效率和较低的温室气体排放量，燃料电池在住宅中的应用在日本受到了关注．另外，太阳能电池系统也早已被导入住宅的能源利用中．然而，住宅用燃料电池和太阳能电池系统的初始投资成为这类住宅能源系统普及应用的一个主要的障碍．在人类的生产活动中，新的产品会随着技术的提高、产量的增加而降低成本，这两种系统的初始投资也必将降低、但是很少有研究涉及到考虑初始投资递减的这类系统的经济性评价．文中通过应用学习曲线的方法，预测了2005—2030年间以5年为间隔的日本住宅用燃料电池和太阳能发电的初始投资递减，并且试算了日本典型住宅的通用能源系统、燃料电池系统和太阳能电池系统的年间能源费用，由此评价了住宅用燃料电池和太阳能发电的经济性．     

DESIGN OF A SOFTWARE AGENT FOR BUSINESS GAMING SIMULATION

1. Introduction 1.1 Gaming Simulation Gaming Simulation is categorized into the following five fields: Entertainment games, Educational games, Experimental games, Research games, and Operational games (Stahl, 1983). Business game is one of the Educational games. Business game is a tool that focuses on understanding about business and the principle of management through the experience in a virtual world. Through this method the players have roles of managers or presidents of companies (Ter…