人胰岛素原乳腺表达载体的构建

通过ＰＣＲ方法扩增了人胰岛素（ＨＩ）基因１．３４ｋｂ的ＤＮＡ片段并克隆于ｐＵＣ１８的ＳｍａⅠ位点内。经ＤＮＡ序列分析表明，该片段为ＨＩ基因的氨基酸编码区及３＇端调控区序列。同时，应用ＰＣＲ方法对牛α－乳白蛋白（ＢαＬＡ）基因进行了扩增，得到了０．８４ｋｂＤＮＡ扩增片段。将其克隆于去除了ＥｃｏＲＩ位点的ｐＵＣ１８ＳｍａＩ位点内，经内切酶分析和ＤＮＡ测序证明该片段是ＢαＬＡ基因５＇调控区序列。ＥｃｏＲ     

迹为1 的n 扩张T C 结构矩阵*

本文证明了：如果Ａ是ｎ阶迹为１的ＴＣ结构矩阵，那么Ａ是ｎ－扩张的当且仅当Ａ满足（１）Ｄ＝Ｄ（Ｊｎ－Ａ）是传递有向图；（２）设ｉ是主对角线上元素为１的下标及Ｅ〈ｎ〉／｛ｉ｝，从顶点ｉ到Ｄ１＝Ｄ（Ｊｎ－１－Ａ「Ｅ」）中的每个顶点最多有一条弧连接。     

边坡抗滑稳定可靠性分析

为了利用一次二阶矩法计算边坡抗滑稳定的可靠指标，针对抗滑稳定分析中的条分法，提出了从极限条件出发，通过在边坡最上面一分条上施加一假想力，使得边坡整体处于极限平衡状态来构造用于Ｂｉｓｈｏｐ法进行可靠性分析的功能函数，并具体给出了基于Ｂｉｓｈｏｐ法进行可靠性的功能函数的推导过程，通过算例与用瑞典条分法、传递系数法进行可靠性分析的结果进行了比较。     

毛细压力对薄液膜蒸发的影响

介绍了一个考虑毛细压力对液膜在垂直平板上蒸发影响的模型．导出了此过程的控制方程组并用可变步长的Ｒｕｎｇｅ－Ｋｕｔｔａ方法进行数值积分．数值结果与Ｎｕｓｓｅｌｔ膜冷凝理论的分析解进行比较，当液膜变得足够薄时，结果与Ｎｕｓｓｅｌｔ膜理论产生显著偏差．这可能是由于Ｎｕｓｓｅｌｔ膜理论没有考虑毛细压力对液膜形状的影响     

合成TiN膜的俄歇电子能谱研究

采用离子束增强沉积方法（ＩＢＥＤ），在Ｆｅ基上制备了ＴｉＮ薄膜。通过划痕仪，透射电镜（ＴＥＭ），俄歇电子能谱（ＡＥＳ），粘着力测量仪的观测，分析和计算，给出了膜的形貌，组分和机械特性；发现ＴｉＮ膜与衬底之间存在一过渡区，膜中含氧量比气相真空沉积（ＣＶＤ）明显减小。在ＡＥＳ定量计算中，本文采用一种与文献不同的方法，较为简便地处理了俄歇谱中Ｔｉ和Ｎ峰的重叠现象     

基于PCA的概率神经网络模式分类方法

研究了概率神经网络隐单元主要分量的选取方法。这些方法的概率神经网络比原来的网络大大降低了隐单元数，并且带来分类测试时间减少的增益。最后，就模拟和实测两组数据进行了计算机仿真，实验结果证实了这种方法的可行性。     

Two classes of metabolites fromTheonella swinhoei are localized in distinct populations of bacterial symbionts

The marine spongeTheonella swinhoei (lithistid Family Theonellidae, Order Astrophorida) has yielded many important, bioactive natural products, most of which share structural features with bacterial natural products. The presence of microbial symbionts inT. swinhoei has been reported, and it was originally suggested that the cytotoxic macrolide swinholide A and many of the bioactive cyclic peptides fromT. swinhoei were all produced by symbiotic cyanobacteria. By transmission electron microscopy, we found four distinct cell populations to be consistently present inT. swinhoei: eukaryotic sponge cells, unicellular heterotrophic bacteria, unicellular cyanobacteria and filamentous heterotrophic bacteria. Purification and chemical analyses of each cell type showed the macrolide swinholide A to be limited to the mixed population of unicellular heterotrophic bacteria, and an anti-fungal cyclic peptide occurred only in the filamentous heterotrophic bacteria. Contrary to prior speculation, no major metabolites were located in the cyanobacteria or sponge cells.     

Effect of various stressors on the levels of lipid peroxide,antioxidants and Na+, K+-ATPase actrivity in rat brain

The level of malondialdehyde (MDA), an index of lipid peroxidation, and the antioxidants superoxide dismutase (SOD) and glutathione (GSH), as well as the activity of Na⁺, K⁺-ATPase, were assessed in whole rat brain after immobilization, anemic hypoxia (NaNO₂) and 72 h starvation. The effect of these stressors on plasma glucose and corticosterone levels was also observed. Hypoxia and starvation stimulated the lipidj peroxide formation in braini as indicated by an increase in the level of MDA, being higher after starvation than hypoxia. Brain SOD activity was also increased in response to hypoxia and starvation while GSH content was only diminished ini hypoxia. However, neither MDA nor antioxidants were affected by immobilization. On the other hand, the activity of brain Na⁺, K⁺-ATPase was significantly increased by immobilization and hypoxia but decreased in starvation. A similar pattern of change was also observed in plasma glucose and corticosterone levels in response to these stressors. These results elucidate differences in the biochemical response of animals towards various types of stress, with increased lipid peroxide formation in hypoxia and starvation.     

Induction of autotomy in the American bird grasshopperSchistocerca americana (Drury) by the ecdysone agonist RH-5849 and investigation of its mode of action

The non-steroidal ecdysone agonist RH-5849 (1,2-dibenzoyl-l-tert-butylhydrazine) was found to be an effective neurotoxicant on injection into the American bird grasshopper,Schistocerca americana (Drury). Treated grasshoppers became immediately hyperactive, followed by loss of coordination, paralysis and eventually death. We also discovered that this compound induced bilateral autotomy of the metathoracic legs during the early stages of intoxication. However, no evidence of ecdysonergic or morphogenetic activities was observed. Synergism studies with neurotoxins of known mode of action suggested that RH-5849 has a mechanism of action similar to that of 4-amino pyridine, which blocks potassium channels.