全文获取类型
收费全文 | 1818篇 |
免费 | 13篇 |
国内免费 | 1篇 |
专业分类
系统科学 | 48篇 |
理论与方法论 | 21篇 |
现状及发展 | 1208篇 |
研究方法 | 73篇 |
综合类 | 410篇 |
自然研究 | 72篇 |
出版年
2020年 | 14篇 |
2018年 | 32篇 |
2017年 | 28篇 |
2016年 | 35篇 |
2015年 | 30篇 |
2014年 | 14篇 |
2013年 | 23篇 |
2012年 | 50篇 |
2011年 | 83篇 |
2010年 | 23篇 |
2009年 | 16篇 |
2008年 | 40篇 |
2007年 | 35篇 |
2006年 | 55篇 |
2005年 | 50篇 |
2004年 | 36篇 |
2003年 | 44篇 |
2002年 | 43篇 |
2001年 | 30篇 |
2000年 | 17篇 |
1999年 | 19篇 |
1992年 | 18篇 |
1991年 | 17篇 |
1985年 | 14篇 |
1984年 | 27篇 |
1983年 | 14篇 |
1982年 | 16篇 |
1981年 | 22篇 |
1979年 | 35篇 |
1978年 | 30篇 |
1977年 | 41篇 |
1976年 | 36篇 |
1975年 | 29篇 |
1974年 | 36篇 |
1973年 | 46篇 |
1972年 | 42篇 |
1971年 | 38篇 |
1970年 | 66篇 |
1969年 | 55篇 |
1968年 | 86篇 |
1967年 | 64篇 |
1966年 | 47篇 |
1965年 | 46篇 |
1964年 | 42篇 |
1963年 | 20篇 |
1962年 | 24篇 |
1961年 | 17篇 |
1959年 | 15篇 |
1958年 | 12篇 |
1957年 | 13篇 |
排序方式: 共有1832条查询结果,搜索用时 343 毫秒
61.
van der Meijden PE Feijge MA Swieringa F Gilio K Nergiz-Unal R Hamulyák K Heemskerk JW 《Cellular and molecular life sciences : CMLS》2012,69(20):3481-3492
The fibrin(ogen) receptor, integrin α(IIb)β(3), has a well-established role in platelet spreading, aggregation and clot retraction. How α(IIb)β(3) contributes to platelet-dependent coagulation is less well resolved. Here, we demonstrate that the potent suppressing effect of clinically used α(IIb)β(3) blockers on tissue factor-induced thrombin generation is linked to diminished platelet Ca(2+) responses and phosphatidylserine (PS) exposure. The same blockers suppress these responses in platelets stimulated with collagen and thrombin receptor agonists, whereas added fibrinogen potentiates these responses. In platelets spreading on fibrinogen, outside-in α(IIb)β(3) signaling similarly enhances thrombin-induced Ca(2+) rises and PS exposure. These responses are reduced in α(IIb)β(3)-deficient platelets from patients with Glanzmann's thrombasthenia. Furthermore, the contribution of α(IIb)β(3) to tissue factor-induced platelet Ca(2+) rises, PS exposure and thrombin generation in plasma are fully dependent on Syk kinase activity. Tyrosine phosphorylation analysis confirms a key role of Syk activation, which is largely but not exclusively dependent on α(IIb)β(3) activation. It is concluded that the majority of tissue factor-induced procoagulant activity of platelets relies on Syk activation and ensuing Ca(2+) signal generation, and furthermore that a considerable part of Syk activation relies on α(IIb)β(3) signaling. These results hence point to a novel role of Syk in integrin-dependent thrombin generation. 相似文献
62.
63.
Antoni Camins Javier G. Pizarro Daniel Alvira Javier Gutierrez-Cuesta Aurelio Vazquez de la Torre Jaume Folch Francesc X. Sureda Ester Verdaguer Felix Junyent Joaquín Jordán Isidre Ferrer Mercè Pallàs 《Cellular and molecular life sciences : CMLS》2010,67(22):3865-3882
In the present study we demonstrated that neurotoxin MPP+-induced DNA damage is followed by ataxia telangiectasia muted (ATM) activation either in cerebellar granule cells (CGC) or in B65 cell line. In CGC, the selective ATM inhibitor KU-55933 showed neuroprotective effects against MPP+-induced neuronal cell loss and apoptosis, lending support to the key role of ATM in experimental models of Parkinson’s disease. Likewise, we showed that knockdown of ATM levels in neuroblastoma B65 cells using an ATM-specific siRNA attenuates the phosphorylation of retinoblastoma protein without affecting other cell-cycle proteins involved in the G0/G1 cell-cycle phase. Moreover, we demonstrated DNA damage, in human brain samples of PD patients. These findings support a model in which MPP+ leads to ATM activation with a subsequent DNA damage response and activation of pRb. Therefore, this study demonstrates a new link between DNA damage by MPP+ and cell-cycle re-entry through retinoblastoma protein phosphorylation. 相似文献
64.
Angela M. Ortega-León Maricela Villagrán-Santa Cruz J. Jamie Zú?iga-Vega Raúl Cueva-del Castillo Fausto R. Méndez-de la Cruz 《西北部美国博物学家》2011,69(1)
Asynchronous reproduction is a common phenomenon in high-elevation populations of lizards from Central México. Sperm storage in the reproductive tract of females is the mechanism for making oocyte fertilization possible. Our study addresses questions related to functional oviductal sperm storage of females mating on different dates throughout the reproductive season. A population of Sceloporus mucronatus with copulation in the summer and ovulation in the fall was chosen for this experiment. Eleven females that copulated in the field during June and 13 females that copulated in captivity during August were maintained in the laboratory until parturition. The number of pregnant females and the litter sizes produced in each experimental group were indicative of the viability of the stored sperm. Sperm stored in the reproductive tract of females were able to fertilize eggs after 4 months. No significant differences were found in the number of pregnant females between the 2 experimental groups nor in the litter sizes that they produced. We found that the amount of time sperm were held in the female reproductive tract (ca. 3 months) had no effect on the capacity of sperm to fertilize eggs. Histological examination of 8 oviducts collected before the mating season eliminated the possibility of sperm storage from one year to the next. In this system, sperm retention could have evolved as a response mechanism to deal with the asynchrony between sexes in the reproductive cycles. However, we cannot rule out alternative hypotheses. 相似文献
65.
Ricardo F. Antunes Cláudia Brandão Gonçalo Carvalho Cristina Girão Fernando A. Arosa 《Cellular and molecular life sciences : CMLS》2009,66(20):3387-3398
Red blood cells (RBC) have emerged as a novel regulatory cell type endowed with bioactivities toward activated human T cells.
Herein we show that the RBC bioactivities act on intracellular pathways initiated by T cell receptor (TCR)-dependent and -independent
stimuli, including IL-2, IL-15, and the mixture of phorbol dibutyrate and ionomycin. The RBC bioactivities preserve the antioxidant
status and are capable of rescuing activated T cells from cell death induced by serum deprivation. They are not mediated by
glycosylphosphatidylinositol-linked receptors or sialic acids, and kinetic studies revealed that they hasten the entrance
into the cell cycle. By using cyclosporine A (CsA) and rapamycin (Rapa) we show that the RBC bioactivities are calcineurin-dependent.
Thus, treatment of T cells with CsA, but not Rapa, impaired RBC bioactivities, and preincubation of RBC with CsA completely
abolished their bioactivities. We have demonstrated that RBC carry out bioactivities that are sensitive to CsA. 相似文献
66.
67.
68.
69.
70.