全文获取类型
收费全文 | 12936篇 |
免费 | 221篇 |
国内免费 | 544篇 |
专业分类
系统科学 | 1454篇 |
丛书文集 | 475篇 |
教育与普及 | 1888篇 |
理论与方法论 | 130篇 |
现状及发展 | 718篇 |
研究方法 | 1047篇 |
综合类 | 7947篇 |
自然研究 | 42篇 |
出版年
2018年 | 47篇 |
2017年 | 65篇 |
2016年 | 63篇 |
2015年 | 74篇 |
2014年 | 148篇 |
2013年 | 91篇 |
2012年 | 559篇 |
2011年 | 727篇 |
2010年 | 256篇 |
2009年 | 91篇 |
2008年 | 629篇 |
2007年 | 748篇 |
2006年 | 903篇 |
2005年 | 1027篇 |
2004年 | 740篇 |
2003年 | 821篇 |
2002年 | 587篇 |
2001年 | 559篇 |
2000年 | 664篇 |
1999年 | 350篇 |
1998年 | 135篇 |
1997年 | 79篇 |
1996年 | 81篇 |
1995年 | 61篇 |
1994年 | 87篇 |
1993年 | 139篇 |
1992年 | 124篇 |
1991年 | 128篇 |
1990年 | 103篇 |
1989年 | 126篇 |
1988年 | 177篇 |
1987年 | 190篇 |
1986年 | 228篇 |
1985年 | 216篇 |
1984年 | 236篇 |
1983年 | 215篇 |
1982年 | 214篇 |
1981年 | 181篇 |
1980年 | 150篇 |
1979年 | 76篇 |
1970年 | 29篇 |
1964年 | 34篇 |
1959年 | 164篇 |
1958年 | 286篇 |
1957年 | 195篇 |
1956年 | 160篇 |
1955年 | 180篇 |
1954年 | 169篇 |
1948年 | 38篇 |
1946年 | 33篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
81.
Natural variation for sulfate content in Arabidopsis thaliana is highly controlled by APR2 总被引:2,自引:0,他引:2
Loudet O Saliba-Colombani V Camilleri C Calenge F Gaudon V Koprivova A North KA Kopriva S Daniel-Vedele F 《Nature genetics》2007,39(7):896-900
Most agronomic traits of importance, whether physiological (such as nutrient use efficiency) or developmental (such as flowering time), are controlled simultaneously by multiple genes and their interactions with the environment. Here, we show that variation in sulfate content between wild Arabidopsis thaliana accessions Bay-0 and Shahdara is controlled by a major quantitative trait locus that results in a strong interaction with nitrogen availability in the soil. Combining genetic and biochemical results and using a candidate gene approach, we have cloned the underlying gene, showing how a single-amino acid substitution in a key enzyme of the assimilatory sulfate reduction pathway, adenosine 5'-phosphosulfate reductase, is responsible for a decrease in enzyme activity, leading to sulfate accumulation in the plant. This work illustrates the potential of natural variation as a source of new alleles of known genes, which can aid in the study of gene function and metabolic pathway regulation. Our new insights on sulfate assimilation may have an impact on sulfur fertilizer use and stress defense improvement. 相似文献
82.
Gout AM;ADPKD Gene Variant Consortium Ravine D Harris PC Rossetti S Peters D Breuning M Henske EP Koizumi A Inoue S Shimizu Y Thongnoppakhun W Yenchitsomanus PT Deltas C Sandford R Torra R Turco AE Jeffery S Fontes M Somlo S Furu LM Smulders YM Mercier B Ferec C Burtey S Pei Y Kalaydjieva L Bogdanova N McCluskey M Geon LJ Wouters CH Reiterova J Stekrová J San Millan JL Aguiari G Del Senno L 《Nature genetics》2007,39(4):427-428
83.
The computational prediction of gene and protein function is rapidly gaining ground as a central undertaking in computational biology. Making sense of the flood of genomic data requires fast and reliable annotation. Many ingenious algorithms have been devised to infer a protein's function from its amino acid sequence, 3D structure and chromosomal location of the encoding genes. However, there are significant challenges in assessing how well these programs perform. In this article we explore those challenges and review our own attempt at assessing the performance of those programs. We conclude that the task is far from complete and that a critical assessment of the performance of function prediction programs is necessary to make true progress in computational function prediction. 相似文献
84.
Mutations in the gene encoding the basal body protein RPGRIP1L, a nephrocystin-4 interactor, cause Joubert syndrome 总被引:7,自引:0,他引:7
Arts HH Doherty D van Beersum SE Parisi MA Letteboer SJ Gorden NT Peters TA Märker T Voesenek K Kartono A Ozyurek H Farin FM Kroes HY Wolfrum U Brunner HG Cremers FP Glass IA Knoers NV Roepman R 《Nature genetics》2007,39(7):882-888
Protein-protein interaction analyses have uncovered a ciliary and basal body protein network that, when disrupted, can result in nephronophthisis (NPHP), Leber congenital amaurosis, Senior-L?ken syndrome (SLSN) or Joubert syndrome (JBTS). However, details of the molecular mechanisms underlying these disorders remain poorly understood. RPGRIP1-like protein (RPGRIP1L) is a homolog of RPGRIP1 (RPGR-interacting protein 1), a ciliary protein defective in Leber congenital amaurosis. We show that RPGRIP1L interacts with nephrocystin-4 and that mutations in the gene encoding nephrocystin-4 (NPHP4) that are known to cause SLSN disrupt this interaction. RPGRIP1L is ubiquitously expressed, and its protein product localizes to basal bodies. Therefore, we analyzed RPGRIP1L as a candidate gene for JBTS and identified loss-of-function mutations in three families with typical JBTS, including the characteristic mid-hindbrain malformation. This work identifies RPGRIP1L as a gene responsible for JBTS and establishes a central role for cilia and basal bodies in the pathophysiology of this disorder. 相似文献
85.
Mutations in amphiphysin 2 (BIN1) disrupt interaction with dynamin 2 and cause autosomal recessive centronuclear myopathy 总被引:4,自引:0,他引:4
Nicot AS Toussaint A Tosch V Kretz C Wallgren-Pettersson C Iwarsson E Kingston H Garnier JM Biancalana V Oldfors A Mandel JL Laporte J 《Nature genetics》2007,39(9):1134-1139
Centronuclear myopathies are characterized by muscle weakness and abnormal centralization of nuclei in muscle fibers not secondary to regeneration. The severe neonatal X-linked form (myotubular myopathy) is due to mutations in the phosphoinositide phosphatase myotubularin (MTM1), whereas mutations in dynamin 2 (DNM2) have been found in some autosomal dominant cases. By direct sequencing of functional candidate genes, we identified homozygous mutations in amphiphysin 2 (BIN1) in three families with autosomal recessive inheritance. Two missense mutations affecting the BAR (Bin1/amphiphysin/RVS167) domain disrupt its membrane tubulation properties in transfected cells, and a partial truncation of the C-terminal SH3 domain abrogates the interaction with DNM2 and its recruitment to the membrane tubules. Our results suggest that mutations in BIN1 cause centronuclear myopathy by interfering with remodeling of T tubules and/or endocytic membranes, and that the functional interaction between BIN1 and DNM2 is necessary for normal muscle function and positioning of nuclei. 相似文献
86.
S. K. MISHRA Shouyang WANG K. K. LAI 《系统科学与复杂性》2007,20(3):344-349
In this paper, we introduce a new class of generalized convex function, namely, a-pseudounivex function, by combining the concepts of pseudo-univex and α-invex functions. Further, we establish some relationships between vector variational-like inequality problems and vector optimization problems under the assumptions of α-pseudo-univex functions. Results obtained in this paper present a refinement and improvement of previously known results. 相似文献
87.
88.
A New Discipline of Science-The Study of Open Complex Giant System and Its Methodology 总被引:2,自引:0,他引:2
Qian XuesenChina Association for Science Technology Beijing ChinaYu JingyuanBeijing Institute of Information Control P.O.Box Beijing ChinaDai RuweiInstitute of Automation Chinese Academy of Sciences Beijing China 《系统工程与电子技术(英文版)》1993,(2)
This paper introduces the conception of open complex giant system and the methodology for dealing with the system, with stress on its profound significance in development of science and technology. The authors conclude that the reductionism underlying the exact science is not suitable to open complex giant system, and the only feasible alternative is the meta-synthetic engineering from the qualitative to the quantitative. 相似文献
89.
A. Farina A. ProtopapaRadar Dpt Selenia S. p. A. Via Tiburtina Km. - Rome Italy 《系统工程与电子技术(英文版)》1992,(3)
After a brief recall of the Sidelobe Canceler (SLC) working principle, including the derivation of a general formula for the Cancellation Ratio (CR), the effects of channel mismatching are investigated. In particular, curves providing CRvalues as a function of amplitude and phase channel mismatching, radar bandwidth, and jammer direction of arrival (JDOA) are provided for the cases of one and two auxiliary antennas. Subsequently, a time -space processor for performance restoration is analyzed in detail. In addition to the above mentioned quantities, the attainable CR value is expressed as a function of the space-time processor parameters. The contribution of the paper is related to the derivation of a number of mathematical equations of CR for several cases of practical interest for the radar engineer. In addition, several curves are presented to assist design of SLC systems. 相似文献
90.
1940年至1980年间,全球的用水量翻了一番,预计到2000年还要再翻一番。由于生活水平的差异,每人每天的生活用水量也相差甚大,为20~500升。全世界有4%的人均日用水量达300~400升(美国为580升),而全世界67%的人口(主要集中在非洲和亚洲)人均日用水量小于50升。时至今日,全球范围内,仍有65%的农村人口,35%的城市人口没有供水系统。尽管如此,在过去的10年里,又有13亿人喝上了卫生的水。但是,在现在52亿的世界人口中,仍有约12亿人喝不上卫生的水。 相似文献