Molecular cloning and expression of interleukin Ibeta (IL-1β) from red seabream (Pagrus major)

The interleukin 1β (IL-1β) cDNA was cloned from the red seabream (Pagrus major) by homology cloning strategy. A cDNA fragment was amplified by PCR using two degenerated primers, which were designed according to the conserved regions of other known IL-1β sequences, and elongated by 3' ends and 5' ends RACE PCR to get the full length coding sequence of red seabream IL-1β (RS IL-1β). The sequence contained 1252 nucleotides that included a 5' untranslated region (UTR) of 84bp, a 3' UTR of 410 bp and an open reading frame (ORF) of 759 nucleotides which could be translated into a putative peptide of 253 amino acids with molecular weight of 28.6 kD and putative isoelectric point pI of 5.29. The deduced peptide contained two potential N-glycosylation sites and an identifiable IL1 family signature, but lacked the signal peptide and the clear ICE cut site, which were common in other nonmammalian IL-1β genes. The RS IL-1β had the highest homology with piscine IL-1β according to phylogenetic tree analysis.     

Simulation of Weld Depth in A-TIG Welding with Unified Arc-electrode model

Ｉｔｋｎｏｗｎｔｈａｔｓｍａｌｌａｍｏｕｎｔｓｏｆｆｌｕｘｏｎｔｈｅｓｕｒｆａｃｅｏｆｓｔａｉｎｌｅｓｓｓｔｅｅｌｃａｎｉｎｃｒｅａｓｅｔｈｅｄｅｐｔｈｏｆｗｅｌｄｐｅｎｅ ｔｒａｔｉｏｎｉｎＴＩＧｗｅｌｄｉｎｇｂｙａｆａｃｔｏｒｏｆｔｈｒｅｅ[1] .Ｔｈｉｓｐｒｏｃｅｓｓｉｓｒｅｆｅｒｒｅｄｔｏａｓ”ＡＴＩＧ”ｏｒＴＩＧｗｅｌｄｉｎｇａｃｔｉ ｖａｔｅｄｂｙｆｌｕｘ .Ｔｈｅｒｅｈａｖｅｂｅｅｎｔｈｒｅｅｐｕｂｌｉｓｈｅｄｐｈｙｓ ｉｃａｌｍｅｃｈａｎｉｓｍｓｔｈａｔａｒｅｐｏｓｓｉｂｌｅｃｏｎｔｒｉ…     

The General Machining Process Simulator GMPS

Ｔｈｅ　Ｇｅｎｅｒａｌ　Ｍａｃｈｉｎｉｎｇ　Ｐｒｏｃｅｓｓ　Ｓｉｍｕｌａｔｏｒ　ＧＭＰＳＸｉａｏＴｉａｎｙｕａｎ，ＹａｎｇＧａｎｇ＆ＬｉＸｉｎ（ＤｅｐａｒｔｍｅｎｔｏｆＡｕｔｏｍａｔｉｏｎ，ＴｓｉｎｇｈｕａＵｎｉｖｅｒｓｉｔｙ，Ｂｅｉｊｉｎｇ，Ｃｈｉ...     

GLOBAL ASYMPTOTIC STABILITY IN N-SPECIES COOPERATIVE SYSTEM WITH TIME DELAYS

ＧＬＯＢＡＬＡＳＹＭＰＴＯＴＩＣＳＴＡＢＩＬＩＴＹＩＮＮ－ＳＰＥＣＩＥＳＣＯＯＰＥＲＡＴＩＶＥＳＹＳＴＥＭＷＩＴＨＴＩＭＥＤＥＬＡＹＳ￥ＣＵＩＪｉｎｇ＇ａｎ（ＤｅｐａｒｔｍｅｎｔｏｆＭａｔｈｅｍａｔｉｃｓ，ＸｉｎｊｉｎｇＵｎｉｖｅｒｓｉｔｙ，Ｕｒｕｍ...     

On the Parsimony of Some Model-Selection Criteria in Econometric Modeling

ＯｎｔｈｅＰａｒｓｉｍｏｎｙｏｆＳｏｍｅＭｏｄｅｌ－ＳｅｌｅｃｔｉｏｎＣｒｉｔｅｒｉａｉｎＥｃｏｎｏｍｅｔｒｉｃＭｏｄｅｌｉｎｇＺＨＡＮＧＷｅｉ；ＬＩＵＢａｏ（ＩｎｓｔｉｔｕｔｅｏｆＳｙｓｔｅｍＥｎｇｉｎｅｅｒｉｎｇ（ＩＳＥ），ＴｉａｎｊｉｎＵｎｉｖ...     

A SYNTHESIS METHOD FOR TWO-ORDER UNCERTAIN SYSTEMS: AUTO-STABLE REGIONAPPROACH

1.IntroductionInrecentyears,thesynthesisproblemforuncertainsystemshasbeendeeplystudied.ManymethodssuchasLyapunov,thevariablestructurecontrol,theadaptivecontrolandtherobustcontrolmethodshavebeenproposed[IMS].Sincetheadaptivecontrolandtherobustcontrolmethod…     

AN IMPROVEMENT ON JORGENS'S ASYMPTOTIC EXMNSION THEOREM FOR TRANSPORT EQUATION

1.IntroductionAfundamentalproblemintransporttheoryisthedeterminationoftheasymptoticbehaviorofthetimedependentsolution.In[if,Jorgenssystematicallyinvestigatedtheneutrontransportequationinafiniteconvexbodywithenergyboundedawayfromtheorigin,andgaveanasymptot…     

The Challenging Topics and Future Directions of the Research in Limnology and Watershed Sciences

Based on reviewing the problems in limnology and watershed sciences in meeting the national demands and the development of theories and methodology, this paper proposed some challenging topics to the sciences, covering the process of lake evolution and the quantitative analysis of human impacts, in -lake nutrient cycling an biogeo-chemical process, the process and mechanisms of material flow in lake-watershed system, digital watershed and the modeling of the surface process of lake -watershed, and ecosystem health and scientific management of lake-watershed.     

AVAILABILITY ANALYSIS OF THE QUEUEING SYSTEM GI/PH/I WITH SERVER BREAKDOWNS

In the existing literature of Repairable Queueing Systems (RQS), i.e., queueing systems with server breakdowns, it is almost all assumed that interarrival times of successive customers are independent, identically exponentially distributed. In this paper, we deal with more generic system GI/PH/1 with server's exponential uptime and phase-type repair time. With matrix analysis theory, we establish the equilibrium condition and the characteristics of the system, derive the transient and stationary availability behavior of the system.     

IS γα≤δ FOR GRAPHS WHICH HAVE DIAMETER TWO?

A subset of S of the vertex set of a graph G is called acyclic if the subgraph it induces in G contains no cycles. S is called an acyclic dominating set of G if it. is both acyclic and dominating. The minimum cardinality of an acyclic dominating set, denoted by 7a(G), is called the acyclic domination number of G. S. M. Hedetniemi et al. on 2000 introduced the concept of acyclic domination and posed the following open problem: Is -ya(G) < <5(G) for any graph whose diameter is two? In this paper, we give a counterexample which disproves the problem.