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To evaluate the influence of the DNA concentration in the aqueous solution on DNA radiation damage, the plasmid DNA in the presence or absence of Mannitol (scavenger of free radical OH.) was irradiated by ^7Li ions and γ rays at various DNA concentrations. Gel electrophoresis analysis revealed that the DNA damage of single and double strand breaks induced by irradiation became more severe at lower DNA concentration. In the condition of γ-ray irradiation, most of double strand breaks (DSB) damage was neutralized and less associated with DNA concentration in the presence of mannitol. However, under ^7Li irradiation, DSB damage could not be cleared by mannitol but was gradually aggravated with decreasing DNA concentrations. These findings imply that under low-LET irradiation, most of the DSB damage is generated by free radical OH·diffusion, and thus may be counteracted by scavengers, while at higher-LET irradiation, quite a fraction of DSB induction is caused by direct ionizing energy deposition of heavy ions, which cannot be eliminated. This work also indicates that the proportion between free radical damage and direct ionizing damage is s constant which is independent of DNA concentration when the DNA concentration is under a certain value (50ng/μL). Our study sheds light on the un- derlying mechanisms in the DNA radiation damage process. 相似文献
993.
教学内容和课程体系的改革是面向 2 1世纪高等教育改革的重要课题。就历史学科而言 ,一、更新观念 ,探索适应社会发展需要的复合型人才培养模式 ;二、课程设置上 ,拓宽基础强化通识教育 ,推行双专业和主辅修制 ,增开选修课 ,以利于人才的培养和就业 ;三、精减、更新教学内容 ,改革、创新教学方法和教学手段 ,使之逐步走上现代化 ,改革考试模式 ,把教学过程与课程论文、基础知识的考核结合起来 ,切实反映学生的综合素质水平 相似文献
994.
This research note focuses on a problem where the cluster sizes for two partitions of the same object set are assumed known;
however, the actual assignments of objects to clusters are unknown for one or both partitions. The objective is to find a
contingency table that produces maximum possible agreement between the two partitions, subject to constraints that the row
and column marginal frequencies for the table correspond exactly to the cluster sizes for the partitions. This problem was
described by H. Messatfa (Journal of Classification, 1992, pp. 5–15), who provided a heuristic procedure based on the linear transportation problem. We present an exact solution
procedure using binary integer programming. We demonstrate that our proposed method efficiently obtains optimal solutions
for problems of practical size.
We would like to thank the Editor, Willem Heiser, and an anonymous reviewer for helpful comments that resulted in improvements
of this article. 相似文献
995.
In order to find the electroencephalogram (EEG) characteristic parameters typical of Alzheimer's disease (AD) and explore an effective diagnostic method, a new mobile current multipole model was proposed to simulate the AD patient's cortical dipole source activities. The indicators of goodness of fit (GOF) and DtononD (DD) were calculated from EEG samples to evaluate the performance of this model. Relevant results showed that this multipole model with higher GOFvalues and larger DD change well fitted the pathological electrical activities of cortical neurons aroused by AD's extended sulcus and gyrus in the cerebral cortex. Meanwhile, the products of DD mean & standard variance were found in a clear linear correlation with the diagnostic data of mini-mental state examination (MMSE) used in AD clinics. Furthermore, by tracing this multipole model's indicators in typical patients and contrasting with the functional magnetic resonance image (fMRI) as AD progressed, we suggested that the DD index may be suitable for monitoring the AD developments as a new diagnostic parameter. 相似文献
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LIANG Wei~ HU Hongying~ GU Xin~ CHE Yuling~ WANG Hui~ GUO Yufeng~ SONG Yudong~ . State Key Laboratory of Freshwater Ecology Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan Hubei China . Environment Simulation Pollution Control State Key Joint Laboratory Department of Environmental Science Engineering Tsinghua University Beijing China 《武汉大学学报:自然科学英文版》2006,11(3):737-741
0 IntroductionMioclroogincuatlri pernotsce pslsaeys afo vrer wyais tmepwoarttaernt t rroelaetmine nbti- .A widerange of micronutrientsis required byactiva-ted sludge[1-5]. Adequate trace nutrient concentra-tions ,including metal ions and vitamins , are re-quiredtosupport all the genera presentinthe activa-ted sludgetreatment systemfor a diverse communityto survive. The lack of specific nutrients may resultin an unbalanced mixed culture,leading to poor-quality effluent , reduced treatment effic… 相似文献
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Djibrilla MOUSSA 《系统科学与系统工程学报(英文版)》2005,14(3):364-380
1. Introduction An early analysis of exchange rate changes and share prices found that the general stock market index tended to move up (down) immediately after a devaluation (revaluation) of the local currency (Giddy 1974). It is surprising that previous researches (Jorion 1990; Amihud 1993; Bodnar and Gentry 1993) find that no contemporaneous relationship between exchange rate changes and excess equity market returns of largest U.S. exporting firms. Recently, it has argued that there is a… 相似文献