New O-superfamily conotoxins fromConus striatus inhabited near Chinese Hainan Island

Conotoxins are short peptide-toxins with specific targets and large diversity. They are useful in analgesia, neuroprotection, detection of some kinds of deseases, and receptor and ion channel study. In order to explore the conotoxin resourses of Chinese oceans, rapid amplification of 3′ cDNA ends (RACE) method was utilized to systemically analyze the O-superfamily conotoxin content ofConus striatus inhabited near Chinese Hainan Island. Six new O-superfamily conopeptides were identified, one of which is highly homologous to MVIIA, an N-type calcium channel antagonist.     

Regulatory role of the sequences downstream fromnodD3 P1 promoter ofRhizobium meliloti

The 660 bp region betweennodD3 P1 promoter and the following coding region ofRhizobium meliloti has been studied. This region is designated “downstream sequences”. It consists of two potential open reading frames, ORF1 and ORF2. Studies on the role of the downstream sequences on the activity ofnocD3 P1 with nod D3(P1)-IacZ fusion show that deletion of the sequences containing ORF2 causes the increase of the activity of the fusion; on the contrary, addition of extra copies of ORF2 markedly decreases the activity of the fusion. These results indicate that the product of ORF2 plays a negative role in the expression ofnod D3.     

Menadione-induced apoptosis and its mechanism in plants

Menadione can induce apoptosis in tobacco protoplasts. Typical characteristics are detected including the condensation of chromatin, the formation of apoptotic bodies and the degradation of genomic DNA into “DNA ladder”. Specific DNase is activated during this process. Ca²⁺ and Mg²⁺ are necessary for its activation, while Zn²⁺ and EDTA (Ethylenediaminetetraacetic acid) can inhibit its activation. The fragmentation of DNA and lamin can be inhibited by DEVD (Ac-Asp -Glu- Val- Asp-aldehyde). The fragmentation of lamin can also be inhibited by PMSF (Phenylmethylsulfonyl fluoride) and CH (Cyclohexinide). These results show that activation of specific DNase and proteases is involved in menadion-induced apoptosis in plants.     

腰部创伤后腰椎稳定性的X线评价

目的 :X线评价腰部创伤后腰椎稳定性。方法 :对 12 8例腰部创伤患者摄腰椎正侧位片 ,应用Denisi等人创伤生物力学实验研究原理方法进行 X线分析 ,对腰椎稳定性作出评价。结果 :腰部创伤后引起椎体及附件骨折 2 0例 ,其中胸 1 1 2例 ,胸 1 2 4例 ,腰 1 2例 ,腰 2～ 5 8例 ,腰椎横突骨折 2例 ,其他附件骨折 2例。腰椎椎体移位 ,全部发生在腰 3～ 5 。腰椎不稳定 8例均有椎体压缩性骨折改变。结论 :腰部创伤除引起椎体及附件骨折外 ,也可致腰椎不稳定。     

肝纤维化的血清学联合检测与B超诊断的对比

目的 :探讨血清透明质酸 (HA)、 型胶原 ( - C)联合检测对肝纤维化诊断的准确性。方法 :运用放射免疫法 (RIA)测定 98例慢性肝炎患者血清 HA、 - C水平 ,并与临床 B型超声检查结果进行对比研究。结果 :二项指标升高与肝纤维组织增生程度呈良好的正相关 (P<0 .0 1)。二项指标分别在慢性肝炎中型、慢性肝炎重型以及肝硬化中渐次升高 ,有显著差异 (P<0 .0 1)。同期 B超检查结果准确率不高。结论 :二项指标的联合检测在肝纤维化和早期肝硬化的诊断上较 B超检查准确、可靠。     

TCDS中基于文件传输路径的响应时间评测

介绍了具有时间约束的分布式软件(TCDS)的可靠性评估方法,阐述了线性文件传输路径的响应时间评估算法;根据这种思想,通过改变配置文件中被测分布式系统的拓扑结构、链路的通信能力和可靠性以及每个程序的所需文件集等信息。能真实模拟现实环境中不同拓扑下的分布式软件．该响应时间评估算法的使用可以减少测试程序的运算时间开销。提高具有时间约束的分布式软件可靠性评测的效率．     

聚变等离子体中的离散阿尔芬波本征模

聚变等离子体中存在负磁剪切的情况下,研究发现在α(α=-q2Rdβ/dr,q是安全因子,β是等离子体压力与磁压力之比,R和r分别代表托卡马克的大半径和小半径)引起的第二个势井里面存在αTAEs(α-induced Alfvén eigenmodes)。这些模式是一种很危险的本征模,容易被激发从而破坏聚变等离子体的稳定状态。对该本征模的研究,为该领域的研究提供了一些参考。     

一种基于频率补偿的GPS位同步方法

针对GPS接收机在跟踪过程中由频率误差引起的位同步概率下降问题,提出了一种基于频率补偿的GPS位同步方法.分析了环路中频率误差引起相干积分累加值衰减的机理,利用不同频率的复信号分别对相干积分结果进行补偿,计算出20个候选位置的值,最大值对应的位置即为数据边界位置.该方法有效减少了由频率误差导致的相干累加损耗,提高了位同步的概率.在弱信号情况下,使用非相干积分方法可进一步提高信号增益和位同步概率.与其他位同步方法相比,所提方法可容忍更大的频率误差,即使跟踪环路处于失锁状态也能准确实现位同步,且在频率误差小于25 Hz的情况下表现出更优的位同步性能.     

Association analyses identify multiple new lung cancer susceptibility loci and their interactions with smoking in the Chinese population

To find additional susceptibility loci for lung cancer, we tested promising associations from our previous genome-wide association study (GWAS) of lung cancer in the Chinese population in an extended validation sample size of 7,436 individuals with lung cancer (cases) and 7,483 controls. We found genome-wide significant (P < 5.0 × 10(-8)) evidence for three additional lung cancer susceptibility loci at 10p14 (rs1663689, close to GATA3, P = 2.84 × 10(-10)), 5q32 (rs2895680 in PPP2R2B-STK32A-DPYSL3, P = 6.60 × 10(-9)) and 20q13.2 (rs4809957 in CYP24A1, P = 1.20 × 10(-8)). We also found consistent associations for rs247008 at 5q31.1 (IL3-CSF2-P4HA2, P = 7.68 × 10(-8)) and rs9439519 at 1p36.32 (AJAP1-NPHP4, P = 3.65 × 10(-6)). Four of these loci showed evidence for interactions with smoking dose (P = 1.72 × 10(-10), P = 5.07 × 10(-3), P = 6.77 × 10(-3) and P = 4.49 × 10(-2) for rs2895680, rs4809957, rs247008 and rs9439519, respectively). These results advance our understanding of lung cancer susceptibility and highlight potential pathways that integrate genetic variants and smoking in the development of lung cancer.     

园林绿化工程苗木移植技术浅析

改善城市居住环境,在较短时间内改善城市景观,需要进行苗木移植.在苗木移植时不采用任何技术措施,种植后只按常规进行淋水管理,树木的成活率就很低.该文概述了苗木栽植原则,分析、归纳了园林建设过程中影响苗木移栽成活率的根系因素和环境因素,并提出了提高园林苗木栽植成活率的对策:(1)做好土壤处理、场地平整及把好苗木质量关等前期准备工作;(2)苗木适时假植;(3)定植前适当修剪与科学定植;(4)分级管养.上述移栽技术经过多年的工程实践证明,能保证树种的成活率在80%以上.