Palaeoclimate: ocean tides and Heinrich events

Climate varied enormously over the most recent ice age--for example, large pulses of ice-rafted debris, originating mainly from the Labrador Sea, were deposited into the North Atlantic at roughly 7,000-year intervals, with global climatic implications. Here we show that ocean tides within the Labrador Sea were exceptionally large over the period spanning these huge, abrupt ice movements, which are known as Heinrich events. We propose that tides played a catalytic role in liberating iceberg armadas during that time.     

Complete protein sequence and identification of structural domains of human apolipoprotein B

Epidemiological, pathological and genetic studies show a strong positive correlation between elevated plasma concentrations of low-density lipoprotein (LDL) cholesterol and the risk of premature coronary heart disease. Apolipoprotein (apo) B-100 is the sole protein component of LDL and is the ligand responsible for the receptor-mediated uptake and clearance of LDL from the circulation. Apo B-100 is made by the liver and is essential for the assembly of triglyceride-rich very low-density lipoproteins (VLDL) in the cisternae of the endoplasmic reticulum and for their secretion into the plasma. VLDL transports triglyceride to peripheral muscle and adipose tissue, where the triglyceride is hydrolysed by lipoprotein lipase. The resultant particle, relatively enriched in cholesteryl ester, constitutes LDL. LDL delivers cholesterol to peripheral tissues where it is used for membrane and steroid hormone biosynthesis and to the liver, the only organ which can catabolize and excrete cholesterol. Plasma LDL levels are therefore determined by the balance between their rate of production from VLDL and clearance by the hepatic LDL (apo B/E) receptor pathway. Here we report the complete 4,563-amino-acid sequence of apo B-100 precursor (relative molecular mass (Mr) 514,000 (514K] determined from complementary DNA clones. Numerous lipid-binding structures are distributed throughout the extraordinary length of apo B-100 and must underlie its special functions as a nucleus for lipoprotein assembly and maintenance of plasma lipoprotein integrity. A domain enriched in basic amino-acid residues has been identified as important for the cellular uptake of cholesterol by the LDL receptor pathway.     

New distributional records for spotted bat ( Euderma maculatum ) in Wyoming

Before 1990 the spotted bat ( Euderma maculatum ) was known in Wyoming from only 2 records. Between June 1994 and May 1997 we documented habitat use and observations incidental to conducting a bat inventory of caves and abandoned mines. By monitoring audible echolocation calls, we observed bats at mist-netting sites. Spotted bats were heard or captured on 13 nights at 10 locations, all of which were near canyons or high cliff faces. No foraging habitat preferences were noted. E. maculatum occurrence appears to be related to roost structure, roost availability, and proximity to a permanent water source.     

Sustainable water resources management: River basin modelling and decision support framework

Sustainability is a key objective of water resources management and this paper describes a modelling and decision support framework that achieves this, illustrated by applications on the UK Thames and Mekong river basins. The decision support framework contains several modules, including an interactive user’s interface linked to a GIS, a geo-database, knowledge base, simulation models and optimization procedures. Based on the analysis of scenarios and proposed interventions, efficient modelling and optimization tools form a comprehensive integrated decision support framework for the analysis and operational management of water resources in the river basin, our emphasis has been on a practical implementation through careful screening of alternatives, consideration of the institutional framework and direct involvement of stakeholders in the decision making process. Operating in this environment is transparent, reproducible and auditable, securing the trust of all interested parties. This paper discusses its applications to water utilisation on the Mekong river basin and drought management of the Lower Thames stored reservoir system.     

Systems microscopy approaches to understand cancer cell migration and metastasis

Cell migration is essential in a number of processes, including wound healing, angiogenesis and cancer metastasis. Especially, invasion of cancer cells in the surrounding tissue is a crucial step that requires increased cell motility. Cell migration is a well-orchestrated process that involves the continuous formation and disassembly of matrix adhesions. Those structural anchor points interact with the extra-cellular matrix and also participate in adhesion-dependent signalling. Although these processes are essential for cancer metastasis, little is known about the molecular mechanisms that regulate adhesion dynamics during tumour cell migration. In this review, we provide an overview of recent advanced imaging strategies together with quantitative image analysis that can be implemented to understand the dynamics of matrix adhesions and its molecular components in relation to tumour cell migration. This dynamic cell imaging together with multiparametric image analysis will help in understanding the molecular mechanisms that define cancer cell migration.     

Circadian organization in reindeer

The light/dark cycle of day and night synchronizes an internal 'biological clock' that governs daily rhythms in behaviour, but this form of regulation is denied to polar animals for most of the year. Here we demonstrate that the continuous lighting conditions of summer and of winter at high latitudes cause a loss in daily rhythmic activity in reindeer living far above the Arctic Circle. This seasonal absence of circadian rhythmicity may be a ubiquitous trait among resident polar vertebrates.