Polygyny,oviposition, life cycle and longevity of the three subspecies of leaf-cutting ants,Acromyrmex subterraneus (Hymenoptera: Formicidae)

ABSTRACT

Research related to the comparative biology of phylogenetically close ant species has received little attention, although it is the basis for understanding the systematics of some sibling groups. The objective was to study the polygyny, oviposition, life cycle and longevity of the three subspecies of leaf-cutting ants. For that, we studied: oviposition rate – queens from three colonies were individually placed in plastic containers, and at 24-hour intervals, the laid eggs were quantified over a period of 96 hours; the workers’ life cycle – the development of immature ants was observed every 24 hours and, as a result, the duration of each stage of development was determined; the workers’ longevity – newly emerged adults were tagged and returned to the fungus chamber of their respective nests, and daily observations were made in each of the nest’s chamber. Laying rates were variable in the three queens studied, with a mean of total eggs laid of 271 for Acromyrmex subterraneus brunneus, 113 for Acromyrmex subterraneus subterraneus and 119 for Acromyrmex subterraneus molestans.The incubation period was 21.2 days in A. s. brunneus, 22.8 days in A. s. subterraneus and 20.4 days in A. s. molestans. The larval phase and pupal phase lasted on average 22.5 and 21.2 days in A. s. brunneus, 22.8 and 15.8 days in A. s. subterraneus and 17.2 and 14.2 days in A. s. molestans, respectively. Life cycle was different in the studied taxa, from 62 to 77 days in A. s. brunneus, from 59–68 days in A. s. subterraneus and from 51 to 55 days in A. s. molestans. The workers’ longevity varied between subspecies A. s. brunneus, A. s. subterraneus and A. s. molestans.     

Pre-ovipositional and ovipositional behaviour of Lasioseius ometes (Oudemans) and Hypoaspis kargi Costa (Acari: Dermanyssiae: Ascidae,Laelapidae) with notes on egg protection strategies in Mesostigmata

Egg deposition by the soil mites Lasioseius ometes (Oudemans, 1903) and Hypoaspis kargi Costa, 1968 was observed using continuous video-monitoring. The process in both species was found to consist of four distinct phases. In Phase 1, both species engage in pre-ovipositional activity prior to the egg being extruded from the genital opening. In L. ometes, the female often selects a substrate depression for egg laying and examines the depression carefully with the gnathosoma and legs I before oviposition. In H. kargi, egg deposition sites are chosen without regard for substrate depressions. Phase 1 lasted approximately 3 minutes for both species. Phase 2 involved egg extrusion and holding of the egg beneath the gnathosoma. Phase 2 lasted approximately 20 times longer in L. ometes (5 minutes) than in H. kargi. Alteration of the egg surface in the form of thorn-like outgrowths was recorded in L. ometes. Egg deposition occurs in Phase 3, and in L. ometes the egg is generally placed at the deposition site using the chelicerae. Hypoaspis kargi also uses the chelicerae for egg manipulation, but also adjusts orientation of the egg with legs I. The sticky nature of egg surface in H. kargi may provide a means for the female to effectively cover the exposed egg with a protective layer of soil particles. Egg deposition in both species occurred in less than 90 seconds. Phase 4 involves egg covering, but behaviour of the female during this last phase differs markedly between the two species. In L. ometes, the female tends to remain close to the egg, whereas in H. kargi the female moves away from the egg to search for substrate particles appropriate for its covering. The average time for Phase 4 in H. kargi was approximately four times longer (80+ minutes) than in L. ometes.     

Nesting biology and behavioural ecology of the solitary bee Monoeca haemorrhoidalis (Smith) and its cleptoparasite Protosiris gigas Melo (Hymenoptera: Apidae: Tapinotaspidini; Osirini)

The species Monoeca haemorrhoidalis, the largest species in the genus, occurs in the Atlantic rainforest of southeastern and southern Brazil. The aim of this study was to investigate the nesting ecology of M. haemorrhoidalis and its interactions with natural enemies. Nest aggregations were studied in an area at the transition between Dense and Mixed Temperate Rainforest, south Brazil. The period of nest construction and cell provisioning started in October and stopped in February. Plant species of the families Orchidaceae, Styracaceae and, mainly, Malpighiaceae, were the most important pollen and floral oil resources that were used in brood cell provisioning. During the nest construction activities, 27 insect species were observed at the nesting sites. The cleptoparasitic bee Protosiris gigas was one of the main causes of M. haemorrhoidalis mortality. Some behavioural and biological data of P. gigas are also reported.     

Species of Antarctoscyphus Peña Cantero,García Carrascosa and Vervoort, 1997 (Cnidaria: Hydrozoa: Symplectoscyphidae) collected by US Antarctic expeditions: biogeographic implications

Antarctoscyphus is one of the most characteristic genera of Antarctic benthic hydroids, with nine of the 10 known species considered to be endemic to the Antarctic; only Antarctoscyphus elongatus is also present in the sub-Antarctic region of Kerguelen. Accordingly, the genus was considered to have an Antarctic–Kerguelen distribution. Here we present the results of the study of the species of Antarctoscyphus collected from different Antarctic areas and from the Magellan region, during several expeditions under the United States Antarctic Research Program between 1958 and 1986. A scanning electron microscopy survey of all known species of the genus was carried out. Eight of the 10 known species of Antarctoscyphus were found in the collection, with A. spiralis and A. elongatus being the most frequently found species, whereas A. biformis, A. fragilis and A. gruzovi were found only once. The type material of A. biformis was reviewed and re-described. This study represents the second records for A. biformis and A. fragilis. The new records allow updating of the biogeographic knowledge on the distribution of several species: A. mawsoni, so far considered endemic to East Antarctica, and A. asymmetricus, considered endemic to West Antarctica, are here considered to have a Circum-Antarctic distribution. Additionally, A. fragilis, previously considered endemic to the Weddell Sea, is now considered to have a West Antarctic–Patagonian distribution, as it was recorded off the Pacific Magellan region. The records gathered here allow us to change the distribution pattern of the genus from Antarctic–Kerguelen to Pan-Antarctic. The study has allowed us to increase the known bathymetric range for some species, some reaching much deeper waters than previously known. Hence, A. fragilis, A. grandis and A. mawsoni, hitherto considered shelf species, are reported from bathyal bottoms of the continental slope. A general discussion on the bathymetric and geographic distribution of all known species is included.

www.zoobank.org/urn:lsid:zoobank.org:pub:1C7ED3F2-3FF1-4C5C-85BB-FAAF557AC2ED     

A revision of the genus Qianleptoneta (Araneae: Leptonetidae)

Southwest China is one of three famous karst areas of the world but research on cave spiders of the area is sparse. A Chinese endemic midget spider genus and species, Qianleptoneta quinquespinata (Leptonetidae) was erected by Chen and Zhu, 2008 Chen, HM and Zhu, MS. 2008. One new genus and species of troglobite spiders (Araneae, Leptonetidae) from Guizhou, China. J Dali Univ., 7(12): 11–14.  [Google Scholar]. Here, based on numerous materials collected from Guizhou Province, the mainly karst area of southwest China, Qianleptoneta is revised. Ten new species are described and illustrated: Q. triangula, Q. nuda, Q. identica, Q. lycotropa, Q. multiseta, Q. lophacantha, Q. robustispina, Q. palmata, Q. sublunata and Q. megaloda. The natural history of each species is presented.     

Phylogenetic relationships of Mesobuthus eupeus (C.L. Koch, 1839) inferred from COI sequences (Scorpiones: Buthidae)

In this study, the first molecular phylogenetic analysis of Mesobuthus eupeus in Iran is presented based on sequence data of a ～ 700-base-pair fragment of cytochrome C oxidase, subunit I. Phylogenetic relationships were inferred using parsimony, maximum likelihood and Bayesian inference. The results support monophyly of M. eupeus, but there is a clear divergence between northern and southern clades. The northern clade includes four subspecies – M. e. eupeus, M. e. philippovitschi, M. e. afghanus and M. e. thersites; whereas the southern clade is comprised of two others – M. e. phillipsi and M. e. kirmanensis. Accordingly, possible scenarios for the evolution and phylogeographic structure of M. eupeus based on the geological history of the Iranian Plateau were proposed. The observation of two distinct lineages supports the proposal that M. eupeus might be a species complex composed of species with highly similar morphological features.     

Infestation levels of Culex (Phytotelmatomyia) mosquitoes inhabiting water-filled Eryngium pandanifolium (Apiaceae) under different sunlight condition

Water accumulations in vegetal structures, known as phytotelmata, serve as aquatic habitats for many insects, including mosquitoes. Among them, Culex (Phytotelmatomyia) is composed by a few species that are highly specialized and poorly known. The objective of this study was to evaluate the association between sunlight conditions and the abundance of immature stages of Culex (Phytotelmatomyia) species in the axils of Eryngium pandanifolium. A forest patch with a dense population of E. pandanifolium in the Lower Delta of the Paraná River (Entre Ríos province, Argentina) was sampled twice a month between December and May. A total of 880 plants were inspected in 220 sample zones defined by a combination of location within the patch and sunlight condition. All individuals collected (n = 521) belonged to Culex (Phytotelmatomyia) hepperi (56%), Culex (Phytotelmatomyia) castroi (40%), and Culex (Phytotelmatomyia) renatoi (4%). According to generalized linear models, the habitat availability (i.e. water presence) was positively associated with sun exposure, but not with the location within the patch. Mosquito presence and abundance were positively related only to water volume. Coexistence of C. hepperi and C. castroi was more frequent than would be expected by chance. Further studies to understand the effects of sunlight and plant architecture on water dynamics are needed.     

Checklist of leaf beetles (Coleoptera: Chrysomelidae) of Turkey,excluding Bruchinae

The leaf beetle fauna of Turkey is reviewed and a complete checklist with provincial distributions is presented for the first time. Seven hundred and seventy-six species are catalogued in total. This represents approximately 22% of the known Palaearctic leaf beetle fauna. The Turkish leaf beetle fauna is characterized by 81 endemic species. Distributions of the species in Turkey are given based on available literature records. Some new materials from new localities are also examined. Taxa in the list are arranged systematically under family, subfamily, genus, subgenus (when recognized) and species. The subspecies are not included in the list but mentioned in the notes given for certain species. The species whose occurrences in Turkey need conformation are not included in the list but are given separately. Additionally, two new synonymies are stated: Psylliodes diversicolor Nadein 2006 = P. kasnakensis Gök et Aslan, 2007 syn.nov.; Phyllotreta maculicornis Pic, 1906 = Ph. ispartaensis Gök, 2005 syn.nov.     

On the systematics,distribution and conservation status of Ichthyophis longicephalus Pillai, 1986 (Amphibia: Gymnophiona: Ichthyophiidae)

The caecilian Ichthyophis longicephalus was described in 1986 based on a single specimen. Only one specimen has been referred subsequently to this species, and the validity of that referral has been questioned. Seven specimens discovered in 2009–2010 at two new localities and two specimens collected in 1990 from a third locality are referred here to I. longicephalus. These specimens are described and compared with the poorly preserved holotype. Mitochondrial DNA data are consistent with the interpretation that the new specimens represent a single species distinct from (and most closely related to I. tricolor among) sampled congeners. The previously referred specimen, from c. 320 km south of the type locality, is not I. longicephalus and probably represents an undescribed species. The “rediscovery” of I. longicephalus in forests and disturbed habitats indicates that the species probably could be transferred from the Data Deficient to the Least Concern category of the IUCN Red List.     

Smicridea McLachlan (Trichoptera: Hydropsychidae) from northwestern Argentina and Bolivia: new species,redescription, association and new records

Four new species of Smicridea (Rhyacophylax) are described from Argentina and Bolivia. Smicridea (Rhyacophylax) bifida sp. nov., S. (R.) elisae sp. nov. and S. (R.) valeni sp. nov. are described from the male; S. (R.) thermophyla sp. nov. is described as the first species of Smicridea from thermal springs. The larva and pupa of S. (R.) thermophila were associated using the metamorphotype method. New distributional records of S. (R.) dithyra, S. (R.) atrobasis, S. (R.) peruana and S. (R.) pampeana are included. Morphological characters of male S. (R.) peruana that were not mentioned before are included. Illustrations of all taxa named in this paper are included.     

Systematics and ecology of the caecilian Crotaphatrema lamottei (Nussbaum) (Amphibia: Gymnophiona: Scolecomorphidae)

New observations on Crotaphatrema lamottei are reported based on fieldwork on Mount Oku between 2006 and 2008. This species was encountered by pitfall trapping and digging, but encounter rates were low. Six new specimens of C. lamottei add substantially to the previous hypodigm for the species and genus, and new morphometric and meristic data are presented. Analysis of mitochondrial DNA sequence data supports strongly the monophyly of Crotaphatrema. The genetic and morphological distance between C. lamottei and Crotaphatrema tchabalmbaboensis is small, although there is a clear difference in colour pattern. The Data Deficient IUCN conservation status of all three species of Crotaphatrema is likely to change most readily as a result of better data on distribution.     

A revision of the ant-like sac spider genus Apochinomma Pavesi 1881 (Araneae: Corinnidae) in the Afrotropical Region

The genus Apochinomma Pavesi, 1881 is revised in the Afrotropical Region. The male and female of the type species, A. formicaeforme Pavesi, 1881, are redescribed. Five new species are described: A. malkini sp. nov. (♂ ♀) from Nigeria, A. parva sp. nov. (♂) from Guineé, and A. tuberculata sp. nov. (♀) from the Ivory Coast in the A. formicaeforme species group, and A. decepta sp. nov. (♂ ♀) from South Africa and Mozambique, and A. elongata sp. nov. (♂) from Botswana, Malawi and Tanzania in the A. decepta species group. Another undescribed species, known only from juveniles, belongs to the latter species group. Members of the A. formicaeforme species group mimic Polyrhachis ants and are thought to be primarily arboreal, whereas the A. decepta species group has ground-dwelling or grass-dwelling representatives that mimic large ground-dwelling ponerine ants. A single case of A. formicaeforme feeding on its model, Polyrhachis gagates, is noted. http://www.zoobank.org/urn:lsid:zoobank.org:pub:04116235-B195-48B0-B27E-076DAF4F51FD     

Co-occurrence of three Aristolochia-feeding Papilionids (Archon apollinus,Zerynthia polyxena and Zerynthia cerisy) in Greek Thrace

Comparative studies of co-occurring species using overlapping resources may help in understanding the mechanisms supporting biotic diversity in species-rich regions, such as the Mediterranean region of Europe. Three Papilionidae butterflies, Archon apollinus, Zerynthia cerisy and Zerynthia polyxena, develop on Aristolochia plants and co-occur in Greek Thrace. We used mark–recapture to describe adult demography and dispersal, and searched for eggs and larvae to assess host plants and microhabitat preferences. Adult flight timing followed a sequence from earliest A. apollinus, through Z. polyxena to late Z. cerisy; this was more prominent in 2010 (warm early spring) than in 2011 (cold delayed spring). Population densities were highest for A. apollinus and lowest for Z. cerisy, whereas dispersal ability followed a reverse pattern. Adults of all three species crossed distances > 3 km and used all habitat types present. Four Aristolochia host plants were used at the study locality: small Aristolochia pallida, intermediate Aristolochia rotunda and Aristolochia hirta, and bulky, late-sprouting Aristolochia clematitis. Both A. apollinus and Z. polyxena used all four Aristolochia species, the former preferring Aristolochia rotunda and Aristolochia hirta, the latter Aristolochia rotunda and Aristolochia pallida. Zerynthia cerisy did not use the early-growing Aristolochia pallida while frequently using the late-growing Aristolochia clematitis. Further parameters affecting oviposition were biotope and canopy closure: early A. apollinus tolerated shady sites but late Z. cerisy avoided them. The simultaneous use of several host plants differing in phenology and habitat requirements, combined with rather high dispersal ability, arguably buffers the butterflies’ population dynamics against yearly variation in weather, while allowing efficient occupation of the diverse Mediterranean landscapes. The regional habitat diversity, created during millennia of human activity, is currently threatened by land abandonment, which may diminish the resource base for the studied butterflies.     

Morphology and natural history of the cryptogenic sponge associate Polydora colonia Moore, 1907 (Polychaeta: Spionidae)

The polychaete Polydora colonia is a widely distributed symbiont of sponges that has been reported as introduced into the Mediterranean Sea. Polydora colonia is re-described based on specimens associated with the sponges Microciona prolifera and Halichondria bowerbanki from New York and aspects of its reproduction and feeding biology are described for the first time. The morphology of P. colonia agrees with previous reports of this species. Females of P. colonia deposited egg capsules (14–19 eggs/capsule) in their tubes on sponges and adelphophagy was observed. Larvae appear to be competent to settle on hosts at the 13-chaetiger stage. One commensal ciliate and one parasitic copepod were found associated with P. colonia. P. colonia as an introduced species is evaluated based on current evidence. Sponge material was observed in the gut of > 50% of the worms examined but further studies are needed to evaluate whether P. colonia is selectively feeding on M. prolifera.     

Four new species of Mesomyzostoma (Myzostomida: Annelida)

Mesomyzostoma Remscheid, 1918 currently includes three described species that live in the coelom and/or gonads of comatulid crinoids: Mesomyzostoma reichenspergeri Remscheid, 1918, Mesomyzostoma katoi Okada, 1933 and Mesomyzostoma lanterbecqae Summers and Rouse, 2014 in Summers, Al-Hakim et al. 2014. Here we describe four new species of Mesomyzostoma and assess their phylogenetic relationships using 18S rRNA, cytochrome oxidase subunit I and 16S rRNA sequence data. We also designate a neotype for M. katoi as the original types appear to be lost. We record M. reichenspergeri from the Australian Great Barrier Reef and from northern Papua New Guinea, but samples from the type locality (Aru Islands, Indonesia) and previously recorded host are needed for confirmation. The new species of Mesomyzostoma are one Japanese species: Mesomyzostoma okadai sp. nov., and three Australian species: Mesomyzostoma lobus sp. nov., Mesomyzostoma leukos sp. nov. and Mesomyzostoma botulus sp. nov. The first infects the coelom of crinoid arms and pinnules, and the other three are found in crinoid oral discs. We also record M. leukos sp. nov. and M. botulus sp. nov. from Papua New Guinea. Phylogenetic analyses suggest that M. okadai sp. nov. is the sister group to all other Mesomyzostoma.     

Protozoan ciliate epibionts on the freshwater apple snail Pomacea figulina (Spix, 1827) (Gastropoda,Ampullariidae) in an urban stream of south‐east Brazil

The objective of this study was to record and analyze the prevalence of ciliated protozoa associated with prosobranchian snails of the species Pomacea figulina collected from an urban stream in south‐eastern Brazil. Four collections were carried out between December, 2005, and March, 2006, from which 23 snails and 10 ‘empty’ shells were obtained. The shells and opercula were scraped over Petri dishes and the ciliates were observed using bright field and phase contrast microscopy. Seven species of ciliates were recorded on the snails of P. figulina. Of the 23 snails analyzed, 82.60% (n = 19) were infested with at least one species of ciliate. No ciliates were found on the ‘empty’ shells and opercula. The results are discussed in terms of ecological aspects involved in this association.     

Helminths of ten species of Litoria frogs (Anura: Hylidae) from Papua New Guinea

One hundred and ninety-one frogs collected in Papua New Guinea from 10 species of the genus Litoria, namely, L. angiana, L. arfakiana, L. caerulea, L. congenita, L. eurynastes, L. gasconi, L. infrafrenata, L. iris, L. kumae and L. modica, were examined for helminths. Eighteen species of helminths were found: one species of Monogenea, Parapolystoma bulliense; three species of Digenea, Diplodiscus amphichrus, Halipegus zweifeli and Mesocoelium monas; 13 species of Nematoda, gravid specimens of Aplectana macintoshii, Cosmocerca novaeguineae, Falcaustra papuensis, Maxvachonia adamsoni, Maxvachonia flindersi, Maxvachonia ingens, Meteterakis crombiei, Moaciria moraveci, Parathelandros mastigurus, Physalopteroides milnensis, Pseudorictularia dipsarilis, Seuratascaris numidica, and larvae (in cysts) of Abbreviata sp. and, one species of Acanthocephala, Pseudoacanthocephalus bufonis. Mean number of helminth species per host species was 3.0 ± 1.4 SD (range 1–5), mean number of helminth species per infected frog was 1.1 ± 0.4 SD (range 1–2), and mean number of helminths per infected host was 33.3 ± 5.8 SD (range 1–36). Twenty-eight new host records and two new locality records are reported. The biogeography of the recovered helminths is discussed.     

Calanoides natalis Brady, 1914 (Copepoda: Calanoida: Calanidae): identity and distribution in relation to coastal oceanography of the eastern Atlantic and western Indian Oceans

Until recently, Calanoides carinatus s.l. was assumed to be very widespread in the upwelling systems of the Atlantic, Indian and western Pacific Oceans. Molecular data, reported here, show that Calanoides from the eastern Atlantic and Arabian Sea are one species and distinct from C. carinatus s.s. The name Calanoides natalis Brady, 1914 is available for this species, which is fully re-described. The illustration by Brady of the male fifth leg and the currently reported genetic data lead us to conclude that C. natalis is distributed from the Bay of Biscay southwards along the eastern Atlantic, around the Cape of Good Hope, and northwards along the western boundary of the Indian Ocean as far as the Arabian Sea. A by-product of this study is the recognition that Calanoides macrocarinatus is a junior synonym of Calanoides brevicornis. Females of C. natalis are easy to distinguish morphologically from C. carinatus s.s. but are more similar to C. brevicornis, apart from size differences. From physical oceanographic evidence we conclude that the Indian Ocean is currently the upstream part of the distribution of C. natalis. Calanoides philippinensis is known to extend into the eastern Indian Ocean at tropical latitudes. It is not known if it is C. philippinensis that has been recorded along the western coast of Australia. An apparently undescribed species of Calanoides occurs on the eastern Australian coast.